J Vet Sci.  2002 Jun;3(2):103-108.

Simultaneous Determination of Various Macrolides by Liquid Chromatography/Mass Spectrometry

• Hwang YH
• Lim JH
• Park BK
• Yun HI

Abstract

Macrolides are frequently used in veterinary medicine as therapeutic and preventive agents for various diseases. It is difficult to determine macrolides simultaneously with conventional methods due to their similar structures. A simultaneous analysis for erythromycin, roxithromycin, tiamulin and tylosin with LC/MS has been developed. Separation was performed on C18 reversed phase column. Mobile phase was gradiently flowed with 10 mM ammonium acetate and methanol. The mass spectrometer was run in the positive mode and selective ion monitoring mode. The molecular ions were [M+H]+ form at m/z 837.5 for erythromycin, at m/z 859.5 for roxithromycin, at m/z 494.2 for tiamulin and at m/z 916.7 for tylosin. Limits of detection were in the range from 0.001 to 0.01 microgram/g lower than their MRLs.

Keyword

simultaneous determination; liquid chromat ography/mass spectrometry; macrolides antibiotics

MeSH Terms

Anti-Bacterial Agents/*analysis
Chromatography, Liquid/*methods
Diterpenes/*analysis
Erythromycin/*analysis
Mass Spectrometry/*methods
Molecular Structure
Roxithromycin/*analysis
Sensitivity and Specificity
Tylosin/*analysis
Veterinary Medicine