J Vet Sci.  2000 Dec;1(2):87-95.

Immunologic reactivity of a lipopolysaccharide-protein complex of type A Pasteurella multocida in mice

• Ryu HI
• Kim CJ

Abstract

The immunologic reactivity of a lipopolysaccharide (LPS)-protein complex isolated from a potassium thiocyanate extract of a Pasteurella multocida (capsular type A and somatic type 3) strain was evaluated in mice. The LPS-protein complex provided 100% protection in mice against a challenge with the homologous strain. However, when the complex was fractionated into LPS and protein moieties by phenol-water treatment, both components lacked immunogenicity. The complex and extracted components were mitogenic for mouse B lymphocytes with the protein moiety the most active. Although immune serum against the LPS-protein complex protected mice against challenge thereby indicating a role for humoral immunity, the LPS-protein complex of P. multocida was also found to induce cell-mediated immunity. This cell-mediated immunity was demonstrated in mice immunized with the complex by: (1). mitogenic responses of T lymphocytes, (2). induction of delayed type hypersensitivity reaction in the hind footpads, and (3). enhanced resistance to challenge infection with Salmonella enteritidis.

Keyword

P. multocida; LPS-protein complex; Immunogenicity

MeSH Terms

Animals
Antibodies, Bacterial/blood/immunology
Bacterial Proteins/chemistry/*immunology
Chemical Fractionation
Hypersensitivity, Delayed
Immune Sera/immunology
Immunity, Cellular
Immunization, Passive
Lipopolysaccharides/chemistry/*immunology
Lymphocyte Activation
Mice
Pasteurella Infections/immunology/*prevention & control
Pasteurella multocida/*chemistry/immunology
Salmonella Infections, Animal/immunology/prevention & control
Salmonella enteritidis/growth & development/immunology
Spleen/cytology/immunology/microbiology