J Korean Med Sci.
2011 Jul;26(7):886-892. 10.3346/jkms.2011.26.7.886.
Spectra of Chromosomal Aberrations in 325 Leukemia Patients and Implications for the Development of New Molecular Detection Systems
- Affiliations
-
- 1Department of Laboratory Medicine, Chonnam National University Hwasun Hospital, Hwasun, Korea. mgshin@chonnam.ac.kr
- 2Genome Research Center for Hematopoietic Diseases, Chonnam National University Hwasun Hospital, Hwasun, Korea.
- 3Brain Korea 21 Project, Center for Biomedical Human Resources at Chonnam National University, Gwangju, Korea.
- 4Environmental Health Center for Childhood Leukemia and Cancer, Chonnam National University Hwasun Hospital, Hwasun, Korea.
- KMID: 1094259
- DOI: http://doi.org/10.3346/jkms.2011.26.7.886
Abstract
- This study investigated the spectrum of chromosomal abnormalities in 325 leukemia patients and developed optimal profiles of leukemic fusion genes for multiplex RT-PCR. We prospectively analyzed blood and bone marrow specimens of patients with acute leukemia. Twenty types of chromosomal abnormalities were detected in 42% from all patients by commercially available multiplex RT-PCR for detecting 28 fusion genes and in 35% by cytogenetic analysis including FISH analysis. The most common cytogenetic aberrations in acute myeloid leukemia patients was PML/PARA, followed by AML1/MGT8 and MLL1, and in acute lymphoid leukemia patients was BCR/ABL, followed by TEL/AML1 and MLL1 gene rearrangement. Among the negative results for multiplex RT-PCR, clinically significant t(3;3)(q21;q26.2), t(8;14)(q24;q32) and i(17)(q10) were detected by conventional cytogenetics. The spectrum and frequency of chromosomal abnormalities in our leukemia patients are differed from previous studies, and may offer optimal profiles of leukemic fusion genes for the development of new molecular detection systems.
MeSH Terms
-
Adaptor Proteins, Signal Transducing/genetics
Adult
Aged
Aged, 80 and over
*Chromosome Aberrations
Chromosomes, Human, Pair 14
Chromosomes, Human, Pair 17
Chromosomes, Human, Pair 3
Chromosomes, Human, Pair 8
Core Binding Factor Alpha 2 Subunit/genetics
Female
Fusion Proteins, bcr-abl/genetics
Gene Rearrangement
Humans
In Situ Hybridization, Fluorescence
Karyotyping
Leukemia/diagnosis/*genetics
Leukemia, Myeloid, Acute/diagnosis/genetics
Male
Middle Aged
Myeloid-Lymphoid Leukemia Protein/genetics
Oncogene Proteins, Fusion/genetics
Precursor Cell Lymphoblastic Leukemia-Lymphoma/diagnosis/genetics
Reverse Transcriptase Polymerase Chain Reaction
- Full Text Links
-
- Actions
-
Cited
- CITED
-
- Close
- Share
-
- Similar articles
-
- Review of Chromosomal Analyses Performed in a Single Hospital
- Assessment of Chromosomal Analyses of 1,180 Cases Suspected of Chromosomal Aberrations
- Changing the frequency and spectra of chromosomal aberrations in Korean patients with acute leukemia in a tertiary care hospital
- Additional cytogenetic aberrations in chronic myeloid leukemia: a single-center experience in the Middle East
- Chromosomal Aberrations and Molecular Cytogenetics in Cancer
