J Vet Sci.  2007 Sep;8(3):303-305. 10.4142/jvs.2007.8.3.303.

Survival of avirulent thermostable Newcastle disease virus (strain I-2)in raw, baked, oiled, and cooked white rice at ambient temperatures

Affiliations
  • 1Department of Veterinary Microbiology and Parasitology, Faculty of Veterinary Medicine, Sokoine University of Agriculture, P. O. Box 3019, Morogoro, Tanzania. phil_wambura@yahoo.com, pwambura@suanet.ac.tz
  • 2School of Veterinary Science, University of Queensland, Brisbane, QLD 4072, Australia.

Abstract

Raw white rice has not been considered a good carrierfor oral vaccination, probably because of its antiviralactivity. Methods are required to overcome antiviralactivity in raw white rice. This study was carried out todetermine the effects of various treatments of raw whiterice on the survival of strain I-2 of Newcastle diseasevirus. These included cooking and baking the rice ormixing the rice with vegetable oil prior to coating withvaccine virus. The vaccine-coated rice was then stored for30min and 24h, followed by quantitative recovery of thevirus. Thirty min after mixing, uncooked, cooked, andbaked rice, and rice mixed with vegetable oil showed titersof 10(6.2), 10(7.2), 10(6.6), and 10(7.0) EID50/0.1ml, respectively.After storage for 24h at 22-25oC, the titers dropped to10(5.0), 10(6.5), 10(5.0), and 10(6.0) EID50/0.1ml for uncooked,cooked, baked, and oiled rice, respectively.

Keyword

chickens; cooked rice; Newcastle disease; strainI-2; thermostable vaccine

MeSH Terms

Animals
Chick Embryo
Chickens
Cookery
Newcastle Disease/*virology
Newcastle disease virus/growth & development/*physiology
Oryza sativa/chemistry/*virology
Viral Vaccines/chemistry

Reference

1. Alexander DJ. Swayne DE, Glisson JR, Jackwood MW, Pearson JE, Reed WM, editors. Newcastle disease. A Laboratory Manual for the Isolation and Identification of Avian Pathogens. 1998. 4th ed. Kennett Square: American Association of Avian Pathologists, University of Pennsylvania;156–163.
2. Alexander DJ. Alexander DJ, editor. Newcastle disease diagnosis. Newcastle Disease. 1988. Boston: Kluwer Academic;145–160.
Article
3. Biswas HR, Haoque MM, Oxley M, Prodhan MAM. A comparative study on the protection of the indigenous chickens against Newcastle disease induced by Australian NDV4 HR and locally produced conventional vaccines in Bangladesh. Prev Vet Med. 1996. 26:157–164.
Article
4. Copland JW. Newcastle Disease in Poultry: A New Food Pellet Vaccine. 1987. (No. 5):Canberra: Australian Centre for International Agricultural Research;119.
5. Ibrahim AL, Ideris A, Babjee AM. Spradbrow PB, editor. An overview of the use of food-based Newcastle disease vaccine in Malaysia. Newcastle Disease in Village Chickens Control with Thermostable Oral Vaccines. 1992. (No. 39):Canberra: Australian Centre for International Agricultural Research;75–78.
6. Jayawardane GWL, de Alwis MCL, Bandara DAWWDA. Oral vaccination of chickens against Newcastle disease with V4 vaccine delivered on processed grains. Aust Vet J. 1990. 67:364–366.
Article
7. Jayawardane GWL, Spradbrow PB. Mucosal immunity in chickens vaccinated with V4 strain of Newcastle disease virus. Vet Microbiol. 1995. 46:69–77.
Article
8. Reed LJ, Muench LH. A simple method of estimating fifty percent endpoints. Am J Hyg. 1938. 27:493–497.
9. Rehmani SF, Spradbrow PB. The influence of adjuvants on oral vaccination of chickens against Newcastle disease. Vet Microbiol. 1995. 46:63–68.
Article
10. Samuel JL, Bensink Z, Spradbrow PB. Oral vaccination of chickens with the V4 strain of Newcastle disease virus. Cooked and raw white rice as a vehicle. Trop Anim Health Prod. 1993. 25:2–10.
Article
11. Spradbrow P. Spradbrow P, editor. A review of the use of food carriers for the delivery of oral Newcastle disease vaccine. Newcastle Disease in Village Chickens Control with Thermostable Oral Vaccine. 1992. (No. 39):Canberra: Australian Centre for International Agricultural Research;18–20.
12. Spradbrow PB. Newcastle disease in village chickens. Poult Sci Rev. 1993/94. 5:57–96.
13. Spradbrow PB, MacKenzie M, Grimes SE. Recent isolates of Newcastle disease virus in Australia. Vet Microbiol. 1995. 46:21–28.
Article
14. Tantaswasdi U, Danvivatanaporn J, Siriwan P, Chaisingh A, Spradbrow PB. Evaluation of an oral Newcastle disease vaccine in Thailand. Prev Vet Med. 1992. 12:87–94.
Article
15. Tu TD, Phuc KV, Dinh NTK, Quoc DN, Spradbrow PB. Vietnamese trials with a thermostable Newcastle disease vaccine (strain I2) in experimental and village chickens. Prev Vet Med. 1998. 34:205–214.
Article
16. Wambura PN, Kapaga AM, Hyera JMK. Experimental trials with a thermostable Newcastle disease virus (strain I2) in commercial and village chickens in Tanzania. Prev Vet Med. 2000. 43:75–83.
Article
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