J Vet Sci.  2007 Sep;8(3):299-301. 10.4142/jvs.2007.8.3.299.

Identification of single-nucleotide polymorphisms of the prion protein gene in sika deer (Cervus nippon laiouanus)

• Jeong HJ
• Lee JB
• Park SY
• Song CS
• Kim BS
• Rho JR
• Yoo MH
• Jeong YH
• Kim YS
• Choi IS

• KMID: 1090807
• DOI: http://doi.org/10.4142/jvs.2007.8.3.299

Abstract

Polymorphisms of the prion protein gene (PRNP) havebeen detected in several cervid species. In order toconfirm the genetic variations, this study examined theDNA sequences of the PRNP obtained from 33 captivesika deer (Cervus nippon laiouanus) in Korea. A total ofthree single-nucleotide polymorphisms (SNPs) at codons100, 136 and 226 in the PRNP of the sika deer wereidentified. The polymorphic site located at codon 100 hasnot been reported. The SNPs detected at codons 100 and226 induced amino acid substitutions. The SNP at codon136 was a silent mutation that does not induce any aminoacid change. The genotype and allele frequencies weredetermined for each of the SNPs.

Keyword

PRNP; sika deer; single-nucleotide polymorphism

MeSH Terms

Animals
Base Sequence
DNA/chemistry/genetics
Deer/*genetics
Genetic Variation
Molecular Sequence Data
Polymerase Chain Reaction/veterinary
Polymorphism, Single Nucleotide
Prions/*genetics
Sequence Analysis, DNA

Figure

• 

  Fig. 1 Amplification of the sika deer prion gene (PRNP). M; 100 bp DNA ladder, lane 1; 771 bp of the sika deer PRNP.

• 

  Fig. 2 Single-nucleotide polymorphism at codon 100 in the sika deer PRNP. Electropherograms show two genotypes at codon 100 in the sika deer PRNP. Left panel; AGT/AGT (Ser/Ser), right panel; AGT/GGT (Ser/Gly). No GGT/GGT (Gly/Gly) homozygote was detected in this study.

• 

  Fig. 3 Single-nucleotide polymorphism at codon 136 in the sika deer PRNP. The electropherograms demonstrate three genotypes at codon 136 in the sika deer PRNP. Left panel; GCT/GCT (Ala/Ala), middle panel; GCT/GCC (Ala/Ala), right panel; GCC/GCC (Ala/Ala).

• 

  Fig. 4 Single-nucleotide polymorphism at codon 226 in the sika deer PRNP. The electropherograms show three genotypes at codon 226 in the sika deer PRNP. Left panel; CAG/CAG (Gln/Gln), middle panel; CAG/GAG (Gln/Glu), right panel; GAG/GAG (Glu/Glu).

Reference

1. Collinge J, Sidle KC, Meads J, Ironside J, Hill AF. Molecular analysis of prion strain variation and the aetiology of 'new variant' CJD. Nature. 1996. 383:685–690.

2. Hunter N, Goldmann W, Foster JD, Cairns D, Smith G. Natural scrapie and PrP genotype: case-control studies in British sheep. Vet Rec. 1997. 141:137–140.

3. Jewell JE, Conner MM, Wolfe LL, Miller MW, Williams ES. Low frequency of PrP genotype 225SF among free-ranging mule deer (Odocoileus hemionus) with chronic wasting disease. J Gen Virol. 2005. 86:2127–2134.

4. Johnson C, Johnson J, Vanderloo JP, Keane D, Aiken JM, McKenzie D. Prion protein polymorphisms in white-tailed deer influence susceptibility to chronic wasting disease. J Gen Virol. 2006. 87:2109–2114.

5. Kataoka N, Nishimura M, Horiuchi M, Ishiguro N. Surveillance of chronic wasting disease in sika deer, Cervus nippon, from Tokachi district in Hokkaido. J Vet Med Sci. 2005. 67:349–351.

6. Meng LP, Zhao DM, Liu HX, Yang JM, Ning ZY, Wu CD, Han CX. Polymorphisms of the prion protein gene (PRNP) in Chinese domestic sika deer (Cervus nippon hortulorum). Anim Genet. 2005. 36:266–267.

7. O'Rourke KI, Besser TE, Miller MW, Cline TF, Spraker TR, Jenny AL, Wild MA, Zebarth GL, Williams ES. PrP genotypes of captive and free-ranging Rocky Mountain elk (Cervus elaphus nelsoni) with chronic wasting disease. J Gen Virol. 1999. 80:2765–2769.

8. O'Rourke KI, Spraker TR, Hamburg LK, Besser TE, Brayton KA, Knowles DP. Polymorphisms in the prion precursor functional gene but not the pseudogene are associated with susceptibility to chronic wasting disease in white-tailed deer. J Gen Virol. 2004. 85:1339–1346.

9. Williams ES. Chronic wasting disease. Vet Pathol. 2005. 42:530–549.