J Vet Sci.  2009 Mar;10(1):85-87. 10.4142/jvs.2009.10.1.85.

Prevalence of Bartonella henselae and Bartonella clarridgeiae in cats and dogs in Korea

• Kim YS
• Seo KW
• Lee JH
• Choi EW
• Lee HW
• Hwang CY
• Shin NS
• Youn HJ
• Youn HY

• KMID: 1089352
• DOI: http://doi.org/10.4142/jvs.2009.10.1.85

Abstract

Blood, saliva, and nail samples were collected from 54 dogs and 151 cats and analyzed for the presence of Bartonella henselae with a novel nested polymerase chain reaction (PCR) method. Bartonella (B.) henselae was detected in feral cat blood (41.8%), saliva (44.1%), and nail (42.7%) samples. B. henselae was also detected in pet cat blood (33.3%), saliva (43.5%), and nail (29.5%) samples and in pet dog blood (16.6%), saliva (18.5%), and nail (29.6%) samples. Nine samples were infected with B. clarridgeiae and 2 were co-infected with B. henselae and B. clarridgeiae of blood samples of dogs. This report is the first to investigate the prevalence of B. henselae and B. clarridgeiae in dogs and cats in Korea, and suggests that dogs and cats may serve as potential Bartonella reservoirs.

Keyword

Bartonella; cats; cat-scratch disease; dogs; Korea

MeSH Terms

Animals
Bartonella/*classification
Bartonella Infections/blood/epidemiology/microbiology/*veterinary
Cat Diseases/blood/epidemiology/*microbiology
Cats
Disease Reservoirs/veterinary
Dog Diseases/epidemiology/*microbiology
Dogs
Hoof and Claw/microbiology
Korea/epidemiology
Prevalence
Saliva/microbiology

Figure

• 

  Fig. 1 Bartonella (B.) henselae (S2) and B. clarridgeiae (S1) nested PCR amplification bands from 2 cats. The negative control band (S3) is visualized on the right side. M: standard maker.

Reference

1. Bergmans AM, de Jong CM, van Amerongen G, Schot CS, Schouls LM. Prevalence of Bartonella species in domestic cats in The Netherlands. J Clin Microbiol. 1997. 35:2256–2261.

2. Branley J, Wolfson C, Waters P, Gottlieb T, Bradbury R. Prevalence of Bartonella henselae bacteremia, the causative agent of cat scratch disease, in an Australian cat population. Pathology. 1996. 28:262–265.

3. Chomel BB, Carlos ET, Kasten RW, Yamamoto K, Chang CC, Carlos RS, Abenes MV, Pajares CM. Bartonella henselae and Bartonella clarridgeiae infection in domestic cats from The Philippines. Am J Trop Med Hyg. 1999. 60:593–597.

4. Chomel BB, Mac Donald KA, Kasten RW, Chang CC, Wey AC, Foley JE, Thomas WP, Kittleson MD. Aortic valve endocarditis in a dog due to Bartonella clarridgeiae. J Clin Microbiol. 2001. 39:3548–3554.

5. Chung JY, Han TH, Kim BN, Yoo YS, Lim SJ. Detection of Bartonella henselae DNA by polymerase chain reaction in a patient with cat scratch disease: a case report. J Korean Med Sci. 2005. 20:888–891.

6. Engvall EO, Brändström B, Fermér C, Blomqvist G, Englund L. Prevalence of Bartonella henselae in young, healthy cats in Sweden. Vet Rec. 2003. 152:366–369.

7. Gundi VA, Bourry O, Davous B, Raoult D, La Scola B. Bartonella clarridgeiae and B. henselae in dogs, Gabon. Emerg Infect Dis. 2004. 10:2261–2262.

8. Heller R, Artois M, Xemar V, De Briel D, Gehin H, Jaulhac B, Monteil H, Piemont Y. Prevalence of Bartonella henselae and Bartonella clarridgeiae in stray cats. J Clin Microbiol. 1997. 35:1327–1331.

9. Keret D, Giladi M, Kletter Y, Wientroub S. Cat-scratch disease osteomyelitis from a dog scratch. J Bone Joint Surg Br. 1998. 80:766–767.

10. Marston EL, Finkel B, Regnery RL, Winoto IL, Graham RR, Wignal S, Simanjuntak G, Olson JG. Prevalence of Bartonella henselae and Bartonella clarridgeiae in an urban Indonesian cat population. Clin Diagn Lab Immunol. 1999. 6:41–44.

11. Maruyama S, Nakamura Y, Kabeya H, Tanaka S, Sakai T, Katsube Y. Prevalence of Bartonella henselae, Bartonella clarridgeiae and the 16S rRNA gene types of Bartonella henselae among pet cats in Japan. J Vet Med Sci. 2000. 62:273–279.

12. McGill S, Wesslén L, Hjelm E, Holmberg M, Auvinen MK, Berggren K, Grandin-Jarl B, Johnson U, Wikström S, Friman G. Bartonella spp. seroprevalence in healthy Swedish blood donors. Scand J Infect Dis. 2005. 37:723–730.

13. Molia S, Chomel BB, Kasten RW, Leutenegger CM, Steele BR, Marker L, Martenson JS, Keet DF, Bengis RG, Peterson RP, Munson L, O'Brien SJ. Prevalence of Bartonella infection in wild African lions (Panthera leo) and cheetahs (Acinonyx jubatus). Vet Microbiol. 2004. 100:31–41.

14. Pons I, Sanfeliu I, Quesada M, Anton E, Sampere M, Font B, Pla J, Segura F. Prevalence of Bartonella henselae in cats in Catalonia, Spain. Am J Trop Med Hyg. 2005. 72:453–457.

15. Rampersad JN, Watkins JD, Samlal MS, Deonanan R, Ramsubeik S, Ammons DR. A nested-PCR with an Internal Amplification Control for the detection and differentiation of Bartonella henselae and B. clarridgeiae: an examination of cats in Trinidad. BMC Infect Dis. 2005. 5:63.

16. Rolain JM, Locatelli C, Chabanne L, Davoust B, Raoult D. Prevalence of Bartonella clarridgeiae and Bartonella henselae in domestic cats from France and detection of the organisms in erythrocytes by immunofluorescence. Clin Diagn Lab Immunol. 2004. 11:423–425.

17. Sander A, Bühler C, Pelz K, von Cramm E, Bredt W. Detection and identification of two Bartonella henselae variants in domestic cats in Germany. J Clin Microbiol. 1997. 35:584–587.

18. Tsukahara M, Tsuneoka H, Iino H, Ohno K, Murano I. Bartonella henselae infection from a dog. Lancet. 1998. 352:1682.

19. Windsor JJ. Cat-scratch disease: epidemiology, aetiology and treatment. Br J Biomed Sci. 2001. 58:101–110.

20. Yamamoto K, Chomel BB, Kasten RW, Hew CM, Weber DK, Lee WI, Koehler JE, Pedersen NC. Infection and re-infection of domestic cats with various Bartonella species or types: B. henselae type I is protective against heterologous challenge with B. henselae type II. Vet Microbiol. 2003. 92:73–86.