J Vet Sci.  2009 Mar;10(1):9-13. 10.4142/jvs.2009.10.1.9.

Distribution and ontogeny of gastrin- and serotonin-immunoreactive cells in the proventriculus of developing chick, Gallus gallus domestica

• Aksoy A
• Cinar K

• KMID: 1089340
• DOI: http://doi.org/10.4142/jvs.2009.10.1.9

Abstract

The ontogeny and distribution of gastrin- and serotonin-immunoreactive (IR) cell in the proventriculus of chicks (Gallus gallus domestica, n = 60) in different growth periods was examined immunohistochemically using antisera specific to gastrin and serotonin. Gastrin and serotonin-IR cells were detected in chick proventriculus. Gastrin-IR cells were first evident after 12 days of incubation in lamina epithelialis and compound glands, while serotonin-IR cells were observed only in compound glands at that same time. Gastrin-IR and serotonin-IR cells increased in frequency on incubation day 14 and 16, respectively. Towards the end of incubation, gastrin- and serotonin-IR cell numbers decreased. In adult chicken, both IR cells were present but not lower numbers. The observations demonstrate the presence of gastrin- and serotonin-IR cells in the proventriculus of developing chicks in temporally changing frequencies.

Keyword

chick; endocrine cell; gastrin; ontogeny; proventriculus; serotonin

MeSH Terms

Animals
Chick Embryo/*metabolism
Endocrine Cells/cytology/metabolism
Gastrins/*metabolism
Gene Expression Regulation, Developmental/physiology
Proventriculus/*embryology/*metabolism
Serotonin/*metabolism

Figure

• 

  Fig. 1 The superficial epithelium of the proventriculus. The superficial epithelium of proventriculus, which was lined by a pseudostratified columnar-like epithelium on day 9 of incubation (A), varied to simple columnar epithelium by day 14 of incubation (B). mct: mesenchymal connective tissue, ep: epithelium, lct: loose connective tissue. Masson trichrome stain. ×1,200.

• 

  Fig. 2 Histological structure of proventriculus wall in different development stages. Primordial glands that were initially evident as an epithelial invagination on day 9 of incubation (A), appearred branching by day 11 of incubation (B). With development, the enlargement in the size of the stomach glands and the increase in thickness of the proventriculus wall were seen clearly in chicks in hatching time (C) and in adults (D). ep: epithelium, mct: mesenchymal connective tissue, lct: loose connective tissue, pg: primordial gland, sg, stomach gland, tm: tunica muscularis. Arrow indicates epithelial invagination constituting stomach glands in the next development stages. Masson trichrome stain. A, ×400, B, ×220, C, ×150, D, ×60.

• 

  Fig. 3 Localization of gastrin- and serotonin-immunoreactive (IR) cells. Gastrin-IR cells were evident in the proventriculus surface epithelium (A) and compound gland (B) on day 12 of incubation. (C) Serotonin-IR cells were evident in the compound gland beginning from day 12 of incubation. In adults, gastrin- (D) and serotonin-IR cells (E) were present in epithelium and compound glands, respectively. Arrows indicate gastrin- or serotonin-IR cells. Avidin-biotin-peroxidase method. A, B and D, ×1,300, C, ×800, E, ×1,500.

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