Korean J Ophthalmol.  2008 Dec;22(4):276-277. 10.3341/kjo.2008.22.4.276.

Transforming growth factor-beta Does Not Induce Endothelin-1 Secretion in Primary Cultured Human Tenon's Fibroblasts

• Hong S
• Kim CY
• Lee JB
• Yoko I
• Seong GJ

• KMID: 1084219
• DOI: http://doi.org/10.3341/kjo.2008.22.4.276

Abstract

No abstract available.

MeSH Terms

Blotting, Western
Cells, Cultured
Connective Tissue Cells/drug effects/metabolism
Endothelin-1/*biosynthesis
Fibroblasts/*drug effects/metabolism
Humans
Transforming Growth Factor beta1/*pharmacology

Figure

• 

  Fig. 1 Representative Western immunoblots. (A) collagen type I, (B) fibronectin, (C) endothelin-1, and (D) beta-actin.

• 

  Fig. 2 Intensity of bands in Western immunoblots. Intensity of each band was calculated as compared with that of 0 hr time point.

Reference

1. Kay EP, Lee HK, Park KS, Lee SC. Indirect mitogenic effect of transforming growth factor-beta on cell proliferation of subconjunctival fibroblasts. Invest Ophthalmol Vis Sci. 1998. 39:481–486.

2. Lama PJ, Fechtner RD. Antifibrotics and wound healing in glaucoma surgery. Surv Ophthalmol. 2003. 48:314–346.

3. Mead AL, Wong TT, Cordeiro MF, et al. Evaluation of anti-TGF-beta2 antibody as a new postoperative anti-scarring agent in glaucoma surgery. Invest Ophthalmol Vis Sci. 2003. 44:3394–3401.

4. Nakamura H, Siddiqui SS, Shen X, et al. RNA interference targeting transforming growth factor-beta type II receptor suppresses ocular inflammation and fibrosis. Mol Vis. 2004. 10:703–711.

5. CAT-152 0102 Trabeculectomy Study Group. Khaw P, Grehn F, et al. A phase III study of subconjunctival human anti-transforming growth factor beta(2) monoclonal antibody (CAT-152) to prevent scarring after first-time trabeculectomy. Ophthalmology. 2007. 114:1822–1830.

6. Fant ME, Nanu L. Human placental endothelin: expression of endothelin-1 mRNA by human placental fibroblasts in culture. Mol Cell Endocrinol. 1995. 109:119–123.

7. Eddy AA. Molecular insights into renal interstitial fibrosis. J Am Soc Nephrol. 1996. 7:2495–2508.

8. Trompezinski S, Pernet I, Mayoux C, et al. Transforming growth factor-beta1 and ultraviolet A1 radiation increase production of vascular endothelial growth factor but not endothelin-1 in human dermal fibroblasts. Br J Dermatol. 2000. 143:539–545.

9. Shi-Wen X, Rodríguez-Pascual F, Lamas S, et al. Constitutive ALK5-independent c-Jun N-terminal kinase activation contributes to endothelin-1 overexpression in pulmonary fibrosis: evidence of an autocrine endothelin loop operating through the endothelin A and B receptors. Mol Cell Biol. 2006. 26:5518–5527.

10. Shi-wen X, Kennedy L, Renzoni EA, et al. Endothelin is a downstream mediator of profibrotic responses to transforming growth factor beta in human lung fibroblasts. Arthritis Rheum. 2007. 56:4189–4194.