J Vet Sci.  2011 Sep;12(3):203-207. 10.4142/jvs.2011.12.3.203.

Cytotoxicity of gamma-ray in rat immature hippocampal neurons

Affiliations
  • 1Department of Veterinary Anatomy, College of Veterinary Medicine, Chonnam National University, Gwangju 500-757, Korea. moonc@chonnam.ac.kr
  • 2Department of Veterinary Toxicology, College of Veterinary Medicine, Chonnam National University, Gwangju 500-757, Korea.
  • 3Laboratory of Experimental Radiology, Research Center, Dongnam Institute of Radiological & Medical Science (DIRAMS), Busan 619-753, Korea.
  • 4Department of Veterinary Anatomy, College of Veterinary Medicine and Applied Radiological Science Research Institute, Jeju National University, Jeju 690-756, Korea.

Abstract

This in vitro study evaluated the detrimental effect of acute gamma (gamma)-irradiation on rat immature hippocampal neurons. Rat immature hippocampal neurons (0.5 day in vitro) were irradiated with 0~4 Gy gamma-rays. Cytotoxicity was analyzed using a lactate dehydrogenase release assay at 24 h after gamma-irradiation. Radiation-induced cytotoxicity in immature hippocampal neurons increased in a dose-dependent manner. Pre-treatments of pro-apoptotic caspase inhibitors and anti-oxidative substances significantly blocked gamma-irradiation-induced cytotoxicity in immature hippocampal neurons. The results suggest that the caspase-dependent cytotoxicity of gamma-rays in immature hippocampal cultured neurons may be caused by oxidative stress.

Keyword

caspase; cytotoxicity; gamma-ray; hippocampal neurons; oxidative stress
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