J Vet Sci.  2006 Jun;7(2):123-125. 10.4142/jvs.2006.7.2.123.

Effect of Fasciola gigantica excretory secretory antigen on rat hematological indices

• Ganga G
• Varshney J
• Sharma RL

• KMID: 1059196
• DOI: http://doi.org/10.4142/jvs.2006.7.2.123

Abstract

The present study was undertaken to investigate the effect of Fasciola gigantica excretory secretory antigen (Fg-ESA) on rat hematological indices. Fg-ESA was prepared by keeping thoroughly washed 40 F. gigantica flukes in 100 ml phosphate buffer saline (PBS) for 2 h at 37degrees C, and centrifuging the supernatant at 12,000 g at 4 degrees C for 30 min. The protein content of Fg-ESA was adjusted to 1.8 mg/ml. The rats were randomly divided into two groups of six rats each. Rats in group A received 0.5 ml of Fg-ESA intraperitoneally (i.p.) for 7 days, whereas control rats in group B received 0.5 ml of PBS i.p. for 7 days. Hemograms of both groups were studied initially and on days 0, 2, 4, 14 and 21 after the final injection of Fg-ESA or PBS. Progressive and significant (p < 0.01) declines in the values of hemoglobin, hematocrit, and total erythrocyte count were observed without significant (p > 0.05) changes in the values of mean corpuscular hemoglobin, mean corpuscular hemoglobin concentration, or mean corpuscular volume in group A. Thus, we conclude that Fg-ESA induces normocytic normochromic anemia in rats.

Keyword

excretory secretory antigen; Fasciola gigantica; hematocrit; hemoglobin; rat; total erythrocyte count

MeSH Terms

Animals
Antigens, Helminth/*pharmacology
Erythrocytes/drug effects/*metabolism
Fasciola/*metabolism
Female
Helminth Proteins/*pharmacology
Hematocrit
Hemoglobins/metabolism
Male
Rats
Time Factors

Reference

1. Cameron TWM. The parasite of Domestic Animals. A Manual for Veterinary Students and Surgeons. 1951. London: Adam and Charles Black;173.

2. Coles EH. Coles EH, editor. Determination of packed cell volume. Veterinary Clinical Pathology. 1980. Philadelphia: Saunders;83–84.

3. Dargie JD. Taylor AER, Muller R, editors. Applications of radio-isotopic techniques to studies of red cell and plasma protein metabolism in helminth diseases of sheep. Pathogenic Process in Parasitic Infection. 1975. Oxford: Blackwell;1–26.

4. Dargie JD, Mulligan W. The onset and development of anaemia and hypoalbuminaemia in rabbits infected with Fasciola hepatica. J Comp Pathol. 1971. 81:187–202.

5. Isseroff H, Spengler RN, Charnok DR. Fascioliasis: similarities of the anemia in rats to that produced by infused proline. J Parasitol. 1979. 65:709–714.

6. Jain NC. Schalm's Veterinary Hematology. 1986. 4th ed. Philadelphia: Lea & Febiger;36–53.

7. Lapage G. Veterinary Parasitology. 1956. Edinburg: Oliver & Boyd;275.

8. Masih DT, Laura C, Casado MJ. Modification of accessory activity of peritoneal cells from Fasciola hepatica infected rats. Vet Immunol Immunopathol. 1996. 53:257–268.

9. Molina EC. Serum interferon-gamma and interleukins-6 and -8 during infection with Fasciola gigantica in cattle and buffaloes. J Vet Sci. 2005. 6:135–139.

10. Snedecor GW, Cochran WG. Statistical Methods. 1989. 8th ed. Ames: Iowa State University Press;26–102.

11. Spengler RN, Isseroff H. Fascioliasis: Is the anemia caused by hematophagia? J Parasitol. 1981. 67:886–892.