Korean J Parasitol.  2004 Jun;42(2):81-84. 10.3347/kjp.2004.42.2.81.

Immunolocalization of the 150 kDa protein in cyst fluid of Taenia solium metacestodes

• Yang HJ
• Chung YB

• KMID: 1051424
• DOI: http://doi.org/10.3347/kjp.2004.42.2.81

Abstract

The 150 kDa protein of cyst fluid (CF) of Taenia solium metacestodes was purified by ammonium sulfate fractionation and Superose 6 HR gel filtration chromatography. The purified protein consisted of three subunits (15, 10 and 7 kDa proteins), which were analyzed with the use of a 7.5-15% gradient sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). Immunofluorescence study was carried out by using immunize specific polyclonal antibody. Positive reactions were noticed at bladder walls, calcareous corpuscles, granules of cyst fluid and some host tissue surrounding the bladder wall of the metacestodes. These results suggest that the 150 kDa protein was secreted into host tissues, inducing immune responses in the host, and it may play important roles in the cellular physiology of the parasites.

Keyword

Taenia solium metacestode; cyst fluid; immunofluorescence

MeSH Terms

Animals
Chemical Fractionation
Chromatography, Gel
Cyst Fluid/*chemistry/metabolism/parasitology
Cysticercosis/*metabolism/parasitology
Electrophoresis, Polyacrylamide Gel
Helminth Proteins/chemistry/*isolation & purification
Mice
Mice, Inbred BALB C
Microscopy, Fluorescence
Molecular Weight
Support, Non-U.S. Gov't
Swine
Swine Diseases/parasitology
Taenia solium/*metabolism