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Classical natural ovine scrapie prions detected in practical volumes of blood by lamb and transgenic mouse bioassays

Dassanayake RP, Truscott T, Zhuang D, Schneider , Madsen-Bouterse SA, Young AJ, Stanton JB, Davis WC, O'Rourke K

Scrapie is diagnosed antemortem in sheep by detecting misfolded isoforms of prion protein (PrP(Sc)) in lymphoid follicles of the rectal mucosa and nictitating membranes. Assay sensitivity is limited if (a)...
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Use of flow cytometry to develop and characterize a set of monoclonal antibodies specific for rabbit leukocyte differentiation molecules

Davis WC, Hamilton M

Flow cytometry was used to identify and characterize monoclonal antibodies (mAbs) that react with rabbit leukocyte differentiation molecules (LDM). Screening sets of mAbs, developed against LDM in other species, for...
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Unique features of bovine lymphocytes exposed to a staphylococcal enterotoxin

Park YH, Lee SU, Ferens WA, Samuels S, Davis WC, Fox LK, Ahn JS, Seo KS, Chang BS, Hwang SY, Bohach GA

We previously demonstrated that stimulation of bovine peripheral blood mononuclear cells (PBMCs) with staphylococcal enterotoxin C (SEC), led to an inversion of the CD4(+):CD8(+) T cell ratio and generation of...
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Analysis of the seroprevalence of bovine paratuberculosis and the application of modified absorbed ELISA to field sample testing in Korea

Park KT, Ahn J, Davis WC, Koo HC, Kwon NH, Jung WK, Kim JM, Hong SK, Park YH

Paratuberculosis (PTB) is a major disease problem worldwide, and causes major economic losses in the dairy industry. Although PTB has been reported in Korea, no studies have been conducted to...
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Immunosuppression by T regulatory cells in cows infected with Staphylococcal superantigen

Chang BS, Bohach GA, Lee SU, Davis WC, Fox LK, Ferens WA, Seo KS, Koo HC, Kwon NH, Park YH

  • KMID: 1091208
  • J Vet Sci.
  • 2005 Sep;6(3):247-250.
Our recent study has provided that the in vitro SEC-induced proliferation of bovine T cells is preceded by a period of a non-proliferative immunoregulation of T cells that may be...
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Characterization of lymphocyte subpopulations and major histocompatibility complex haplotypes of mastitis-resistant and susceptible cows

Park YH, Joo YS, Park JY, Moon JS, Kim SH, Kwon NH, Ahn JS, Davis WC, Davies CJ

  • KMID: 1094685
  • J Vet Sci.
  • 2004 Mar;5(1):29-39.
Bovine mastitis is an infectious disease with a major economic influence on the dairy industry worldwide. Many factors such as environment, pathogen, and host affect susceptibility or resistance of an...
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The use of crossreactive monoclonal antibodies to characterize the immune system of the water buffalo (Bubalus bubalis)

Davis WC, Khalid AM, Hamilton M, Ahn JS, Park YH, Cantor GH

  • KMID: 1103516
  • J Vet Sci.
  • 2001 Aug;2(2):103-109.
One of the major difficulties in studying the mechanisms of host defense in economically important species indigenous to Asia and the Middle East is the lack of monoclonal antibody (mAb)...
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Phenotypic and functional analysis of bovine gammadelta lymphocytes.

Park YH, Yoo HS, Yoon JW, Yang SJ, An JS, Davis WC

  • KMID: 1103473
  • J Vet Sci.
  • 2000 Jun;1(1):39-48.
The studies have provided the first comprehensive comparison of the factors regulating activation and proliferation of WC1+ and WC1- gammadelta T cells. The investigation has shown that accessory molecules essential...
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