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Virus Isolation from the First Patient with SARS-CoV-2 in Korea

Park WB, Kwon NJ, Choi SJ, Kang CK, Choe PG, Kim JY, Yun J, Lee GW, Seong MW, Kim NJ, Seo JS, Oh MD

Novel coronavirus (SARS-CoV-2) is found to cause a large outbreak started from Wuhan since December 2019 in China and SARS-CoV-2 infections have been reported with epidemiological linkage to China in...
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Suspension culture of Vero cells for the production of adenovirus type 5

Lee DK, Park J, Seo DW

PURPOSE: Most cell culture processes for viral vaccine production are mainly based on adherent cell culture systems using serum, which are associated with expensive and labor-intensive processes to produce large...
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Generation and protective efficacy of a cold-adapted attenuated genotype 2b porcine epidemic diarrhea virus

Won H, Lee DU, Jang G, Noh YH, Lee SC, Choi HW, Yoon IJ, Yoo HS, Lee C

The recent emergence and re-emergence of porcine epidemic diarrhea virus (PEDV) underscore the urgent need for the development of novel, safe, and effective vaccines against the prevailing strain. In this...
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Isolation and identification of canine adenovirus type 2 from a naturally infected dog in Korea

Yang DK, Kim HH, Yoon SS, Lee H, Cho IS

Canine adenovirus type 2 (CAV-2) infection results in significant respiratory illness in dogs. Isolating and culturing CAV-2 allows for investigations into its pathogenesis and the development of vaccines and diagnostic...
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Isolation and characterization of a new porcine epidemic diarrhea virus variant that occurred in Korea in 2014

Yang DK, Kim HH, Lee SH, Yoon SS, Park JW, Cho IS

Outbreaks of porcine epidemic diarrhea (PED) have resulted in significant economic losses in the swine industry, and another PED outbreak occurred in 2014 in Korea. Isolating and culturing PED virus...
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Efficacy of inactivated variant porcine epidemic diarrhea virus vaccines in growing pigs

Lee SH, Yang DK, Kim HH, Cho IS

PURPOSE: The first aim of this study was to develop a novel inactivated porcine epidemic diarrhea virus (PEDV) vaccine using the recently isolated Korean PEDV QIAP1401 strain and to evaluate...
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In vitro antiviral activity of ribavirin against severe fever with thrombocytopenia syndrome virus

Lee MJ, Kim KH, Yi J, Choi SJ, Choe PG, Park WB, Kim NJ, Oh MD

BACKGROUND/AIMS: Severe fever with thrombocytopenia syndrome (SFTS) is an emerging infectious disease caused by severe fever with thrombocytopenia syndrome virus (SFTSV), a novel bunyavirus. As yet, there is no effective...
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The First Meeting of the National Control Laboratories for Vaccines and Biologicals in the Western Pacific in 2016

Oh H, Shin J, Ato M, Ma X, Williams D, Han K, Kim YJ, Kang H, Jung K, Hanada K, Ochiai M, Van Hung P, Park S, Ahn C

The first meeting of the National Control Laboratories for Vaccines and Biologicals in the Western Pacific Region was held on September 1–2, 2016 in Seoul, the Republic of Korea. The...
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An oral Aujeszky's disease vaccine (YS-400) induces neutralizing antibody in pigs

Yang DK, Kim HH, Choi SS, Hyun BH, Song JY

PURPOSE: Aujeszky's disease (AD) is an economically important disease affecting both wild and domestic pigs of the species Sus scrofa. A previous study yielded serological evidence of AD in Korean...
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Sterculic Acid and Its Analogues Are Potent Inhibitors of Toxoplasma gondii

Hao P, Alaraj IQ, Al Dulayymi JR, Baird MS, Liu J, Liu Q

Toxoplasmosis is a serious disease caused by Toxoplasma gondii, one of the most widespread parasites in the world. Lipid metabolism is important in the intracellular stage of T. gondii. Stearoyl-CoA...
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Improved immunogenicity of Newcastle disease virus inactivated vaccine following DNA vaccination using Newcastle disease virus hemagglutinin-neuraminidase and fusion protein genes

Firouzamandi M, Moeini H, Hosseini D, Bejo MH, Omar AR, Mehrbod P, Ideris A

The present study describes the development of DNA vaccines using the hemagglutinin-neuraminidase (HN) and fusion (F) genes from AF2240 Newcastle disease virus strain, namely pIRES/HN, pIRES/F and pIRES-F/HN. Transient expression...
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Isolation of Middle East Respiratory Syndrome Coronavirus from a Patient of the 2015 Korean Outbreak

Park WB, Kwon NJ, Choe PG, Choi SJ, Oh HS, Lee SM, Chong H, Kim JI, Song KH, Bang JH, Kim ES, Kim HB, Park SW, Kim NJ, Oh MD

During the 2015 outbreak of Middle East respiratory syndrome coronavirus (MERS-CoV) in Korea, 186 persons were infected, resulting in 38 fatalities. We isolated MERS-CoV from the oropharyngeal sample obtained from...
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Antiviral Activity of Chrysin Derivatives against Coxsackievirus B3 in vitro and in vivo

Song JH, Kwon BE, Jang H, Kang H, Cho S, Park K, Ko HJ, Kim H

Chrysin is a 5,7-dihydroxyflavone and was recently shown to potently inhibit enterovirus 71 (EV71) by suppressing viral 3C protease (3Cpro) activity. In the current study, we investigated whether chrysin also...
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Isolation of novel bovine parainfluenza virus type 5 (bPIV5) and its incidence in Korean cattle

Yang DK, Nah JJ, Kim HH, Choi SS, Bae YC, Park JW, Song JY

  • KMID: 2307877
  • Korean J Vet Res.
  • 2014 Jun;54(2):107-112.
Four viruses showing cytopathic effects in MDBK cells were isolated from brains of cattle showing downer cattle syndrome in 2012. The isolates were confirmed to belong to the genus Rubulavirus...
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Antiviral Activity of Hederasaponin B from Hedera helix against Enterovirus 71 Subgenotypes C3 and C4a

Song J, Yeo SG, Hong EH, Lee BR, Kim JW, Kim J, Jeong H, Kwon Y, Kim H, Lee S, Park JH, Ko HJ

  • KMID: 2048329
  • Biomol Ther.
  • 2014 Jan;22(1):41-46.
Enterovirus 71 (EV71) is the predominant cause of hand, foot and mouth disease (HFMD). The antiviral activity of hederasaponin B from Hedera helix against EV71 subgenotypes C3 and C4a was...
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The influence of serum substituents on serum-free Vero cell conditioned culture media manufactured from Dulbecco's modified Eagle medium in mouse embryo culture

Lee JS, Kim JH, Seo YS, Yang JB, Kim YI, Kim HJ, Lee KH

  • KMID: 2313969
  • Obstet Gynecol Sci.
  • 2013 Sep;56(5):320-329.
OBJECTIVE: This study was conducted to examine the influences of supplementation of the serum substituents and available period of serum-free Vero cell conditioned media (SF-VCM) manufactured from Dulbecco's modified Eagle...
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Cell Culture-based Influenza Vaccines as Alternatives to Egg-based Vaccines

Lee I, Kim JI, Park MS

Influenza viruses have raised public health concerns by seasonal epidemics and intermittent pandemics. Vaccination is considered as the most effective method for preventing influenza infection in humans. Current influenza vaccines...
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Serotype- and serogroup-specific detection of African horsesickness virus using phage displayed chicken scFvs for indirect double antibody sandwich ELISAs

van Wyngaardt W, Mashau C, Wright I, Fehrsen J

There is an ongoing need for standardized, easily renewable immunoreagents for detecting African horsesickness virus (AHSV). Two phage displayed single-chain variable fragment (scFv) antibodies, selected from a semi-synthetic chicken antibody...
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Protective effect of methanol extract from citrus press cakes prepared by far-infrared radiation drying on H2O2-mediated oxidative damage in Vero cells

Wijesinghe WAJP, Senevirathne M, Oh MC, Jeon YJ

  • KMID: 2313543
  • Nutr Res Pract.
  • 2011 Oct;5(5):389-395.
In the present study, a suitable drying method was developed for citrus press cakes (CPCs), which are produced as a by-product in citrus juice plants, and the protective effect of...
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The Composition of the Root of Ixeris dentata var. albiflora Nakai. and Cell Viability and DPPH Radical Scavenging Activities of its Extract

Hong S, Jeong D, Kim K, Hwang E

Ixeris dentata var. albiflora Nakai, a herbal plant, is often used to make a strong stomach as an antiphlogistic used when dyspepsia and to improve appetite in Korea and China....
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