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Applications of Organoids for Tissue Engineering and Regenerative Medicine

Yoo JJ, Cho CS, Jo I

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Overcoming Current Dilemma in Cartilage Regeneration: Will Direct Conversion Provide a Breakthrough?

Im Gi, Kim TK

Direct reprogramming/direct conversion/transdifferentiation is a process that induces conversion between completely different matured (differentiated) cells in higher organisms. Unlike the process of reprogramming of differentiated cells into induced pluripotent stem...
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In Vitro Modeling of the Tumor Microenvironment in Tumor Organoids

Devarasetty M, Forsythe SD, Shelkey E, Soker S

BACKGROUND: The tumor microenvironment (TME) represents the many components occupying the space within and surrounding a tumor, including cells, signaling factors, extracellular matrix, and vasculature. Each component has the potential...
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The Application of Decellularized Adipose Tissue Promotes Wound Healing

Xia Z, Guo X, Yu N, Zeng A, Si L, Long F, Zhang W, Wang X, Zhu L, Liu Z

BACKGROUND: Due to adipose-derived stem cells (ASCs) being easy to obtain, their rapid proliferation rate, and their multidirectional differentiation capabilities, they have been widely used in the field of regenerative...
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Engineering Human Brain Organoids: From Basic Research to Tissue Regeneration

Jeong HJ, Jimenez Z, Mukhambetiyar K, Seo M, Choi JW, Park TE

BACKGROUND: Brain organoids are self-organized from human pluripotent stem cells and developed into various brain region following the developmental process of brain. Brain organoids provide promising approach for studying brain...
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Gaining New Biological and Therapeutic Applications into the Liver with 3D In Vitro Liver Models

Lee SW, Jung DJ, Jeong GS

BACKGROUND: Three-dimensional (3D) cell cultures with architectural and biomechanical properties similar to those of natural tissue have been the focus for generating liver tissue. Microarchitectural organization is believed to be...
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Challenges in Perioperative Animal Care for Orthotopic Implantation of Tissue-Engineered Pulmonary Valves in the Ovine Model

Hussein HA, Hussein HA, Sircuta C, Cotoi OS, Movileanu I, Nistor D, Cordos B, Deac R, Suciu H, Brinzaniuc K, Simionescu DT, Harpa MM

BACKGROUND: Development of valvular substitutes meeting the performance criteria for surgical correction of congenital heart malformations is a major research challenge. The sheep is probably the most widely used animal...
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Microfluidic Systems for Assisted Reproductive Technologies: Advantages and Potential Applications

Sequeira RC, Criswell T, Atala A, Yoo JJ

Microfluidic technologies have emerged as a powerful tool that can closely replicate the in-vivo physiological conditions of organ systems. Assisted reproductive technology (ART), while being able to achieve successful outcomes,...
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Studying Adipose Tissue in the Breast Tumor Microenvironment In Vitro: Progress and Opportunities

Mertz D, Sentosa J, Luker G, Takayama S

BACKGROUND: The breast cancer microenvironment contains a variety of stromal cells that are widely implicated in worse patient outcomes. While many in vitro models of the breast tumor microenvironment have...
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Glucose Uptake and Insulin Response in Tissue-engineered Human Skeletal Muscle

Kondash ME, Ananthakumar A, Khodabukus A, Bursac N, Truskey GA

BACKGROUND: Tissue-engineered muscles (‘‘myobundles’’) offer a promising platform for developing a human in vitro model of healthy and diseased muscle for drug development and testing. Compared to traditional monolayer cultures,...
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Hair Follicle as a Source of Pigment-Producing Cells for Treatment of Vitiligo: An Alternative to Epidermis?

Ghasemi M, Bajouri A, Shafiiyan S, Aghdami N

To discuss the advantages and limitations of hair follicle-derived cell transplantation (FCT) in vitiligo, compared to the epidermal cell transplantation (ECT), and the knowledge gap which is required to be...
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Osteoporotic Conditions Influence the Activity of Adipose-Derived Stem Cells

Park JS, Piao J, Park G, Yoo KS, Hong HS

BACKGROUND: Estrogen deficiency decreases bone density and increases the risk of osteoporosis and fracture, thereby necessitating reconstruction of bone regeneration. As bone marrow mesenchymal stem cell (BMSCs) lose viability and...
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Comparative Study on Bone Marrow-Versus Adipose-Derived Stem Cells on Regeneration and Re-Innervation of Skeletal Muscle Injury in Wistar Rats

Moussa MH, Hamam GG, Abd Elaziz AE, Rahoma MA, Abd El Samad AA, El-Waseef DAA, Hegazy MA

BACKGROUND: Skeletal muscle injuries are frequent clinical challenges due to associated fibrosis and disability. Regenerative medicine is an emerging promising strategy for such cases. The aim of this study was to...
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Articular Cartilage Regeneration Utilizing Decellularized Human Placental Scaffold, Mesenchymal Stem Cells and Platelet Rich Plasma

Özdemir E, Emet A, Hashemihesar R, Sinan Yürüker AC, Kılıc E, Çetinkaya DU, Turhan E

BACKGROUND: Articular cartilage repair has been a challenge in orthopedic practice due to the limited self-regenerative capability. Optimal treatment method for cartilage defects has not been defined. We investigated the...
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Synergistic Effect of Laminin and Epidermal Growth Factor on Biological and Morphological Properties of Co-Cultured Myoblasts and Fibroblasts

Mat Afandi MA, Maarof M, Chowdhury SR, Idrus RBH

BACKGROUND: One of the long-standing problems of myoblasts in vitro expansion is slow cell migration and this causes fibroblast population to exceed myoblasts. In this study, we investigated the synergistic...
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