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Enhancement of antigen-specific humoral immune responses and protein solubility through conjugation of bacterial flagellin, Vibrio vulnificus FlaB, to the N-terminus of porcine epidemic diarrhea virus surface protein antigen S0

Oh SH, Kim Cho YS, Lee HB, Lee SM, Kim WS, Hong L, Cho CS, Choi YJ, Kang SK

Porcine epidemic diarrhea (PED) is a highly contagious enteric swine disease. The large economic impact of PED on the swine industry worldwide has made the development of an effective PED...
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A new recombined porcine reproductive and respiratory syndrome virus virulent strain in China

Dong JG, Yu LY, Wang PP, Zhang LY, Liu YL, Liang PS, Song CX

Porcine reproductive and respiratory syndrome (PRRS) is one of the most important swine diseases worldwide. In the present study, a new virulent strain of PRRS virus (PRRSV), GDsg, was isolated...
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Piglet colibacillosis diagnosis based on multiplex polymerase chain reaction and immunohistochemistry of paraffin-embedded tissues

De Lorenzo C, de Andrade CP, Machado VS, Bianchi MV, Rolim VM, Cruz RA, Driemeier

Enterotoxigenic Escherichia coli (ETEC) causes diarrhea in pigs, referred to as colibacillosis. The aim of this study was to optimize multiplex polymerase chain reaction (PCR) and immunohistochemistry (IHC) analyses of...
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Immune responses in pigs and cattle vaccinated with half-volume foot-and-mouth disease vaccine

Park ME, You SH, Lee SY, Lee KN, Ko MK, Choi JH, Kim B, Lee JS, Park JH

With the current commercial foot-and-mouth disease vaccine, inoculating twice increases the formation of denatured meat due to granuloma or residual adjuvant at the injection site in pigs, resulting in economic...
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Duplex nested reverse transcriptase polymerase chain reaction for simultaneous detection of type 2 porcine reproductive and respiratory syndrome virus and porcine circovirus type 2 from tissue samples

Kim HK, Lyoo KS, Huynh TM, Moon HJ, Nguyen VG, Park BK

There are high levels of co-incidence of porcine reproductive and respiratory syndrome virus (PRRSV) and porcine circovirus type 2 (PCV2) in porcine tissue. This study established a duplex nested reverse...
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Sequence analysis of the spike gene of Porcine epidemic diarrhea virus isolated from South China during 2011–2015

Zhao X, Li Z, Zeng X, Zhang G, Niu J, Sun B, Ma J

The spike gene of porcine epidemic diarrhea virus (PEDV) was sequenced from 55 South China field strains isolated from pigs with symptoms of diarrhea. The sequences were compared within the...
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Porcine circovirus type 2 increases interleukin-1beta and interleukin-10 production via the MyD88–NF-kappa B signaling pathway in porcine alveolar macrophages in vitro

Han J, Zhang S, Zhang Y, Chen M, Lv Y

Porcine alveolar macrophages (PAMs) represent the first line of defense in the porcine lung after infection with porcine circovirus type 2 (PCV2) via the respiratory tract. However, PCV2 infection impairs...
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Two clinical isolates of Mycoplasma hyosynoviae showed differing pattern of lameness and pathogen detection in experimentally challenged pigs

Gomes-Neto JC, Raymond M, Bower L, Ramirez A, Madson DM, Strait EL, Rosey EL, Rapp-Gabrielson VJ

Mycoplasma (M.) hyosynoviae is known to colonize and cause disease in growing-finishing pigs. In this study, two clinical isolates of M. hyosynoviae were compared by inoculating cesarean-derived colostrum-deprived and specific-pathogen-free...
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Mycobacterium bovis infection in a wild sow (Sus scrofa): the first case in Korea

Ku BK, Jeon BY, Kim JM, Jang YB, Jang Y, Yu SY, Kim J, Moon OK, Jung SC, Lee MK, Jeong TN

Mycobacterium (M.) bovis causes tuberculosis and has a broad host range, including humans, livestock, and wild animals. M. bovis infection of wild boar has been reported in several European countries....
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Molecular characterization and phylogenetic analysis of pseudorabies virus variants isolated from Guangdong province of southern China during 2013–2014

Fan J, Zeng X, Zhang G, Wu Q, Niu J, Sun B, Xie Q, Ma J

Outbreaks of pseudorabies (PR) have occurred in southern China since late 2011, resulting in significant economic impacts on the swine industry. To identify the cause of PR outbreaks, especially among...
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Seroprevalence and associated risk factors of pseudorabies in Shandong province of China

Hu D, Lv L, Zhang Z, Xiao Y, Liu S

A cross-sectional serological study was conducted in Shandong province of China to determine the seroprevalence and risk factors associated with seropositivity due to pseudorabies virus (PRV) infection in small- and...
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Increased humoral antibody response of foot-and-mouth disease virus vaccine in growing pigs pre-treated with poly-γ-glutamic acid

Lee JH, Kang IJ, Kim AR, Noh YS, Chung HC, Park BK

This study was conducted to determine if humoral antibody response of foot-and-mouth disease (FMD) vaccine improved in 8-week-old growing pigs born to well-vaccinated sows pre-treated with 60 mg of poly-γ-glutamic...
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Identification and Prevalence of Globocephalus samoensis (Nematoda: Ancylostomatidae) among Wild Boars (Sus scrofa coreanus) from Southwestern Regions of Korea

Ahn KS, Ahn AJ, Kim TH, Suh GH, Joo KW, Shin SS

This study describes the first record of Globocephalus samoensis (Nematoda: Ancylostomatidae) recovered in wild boars from southwestern regions of Korea. Gastrointestinal tracts of 111 Korean wild boars (Sus scrofa coreanus)...
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Intrapancreatic ectopic splenic tissue found in a cloned miniature pig

Koo OJ, Ha SK, Park SJ, Park HJ, Kim SJ, Kwon D, Kang JT, Moon JH, Park EJ, Jang G, Lee BC

Somatic cell nuclear transfer (SCNT) is a cost-effective technique for producing transgenic pigs. However, abnormalities in the cloned pigs might prevent use these animals for clinical applications or disease modeling....
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Quantitative real-time polymerase chain reaction for detecting Mycoplasma hyosynoviae and Mycoplasma hyorhinis in pen-based oral, tonsillar, and nasal fluids

Gomes Neto JC, Bower L, Erickson B, Wang C, Raymond M, Strait EL

Mycoplasma (M.) hyorhinis and M. hyosynoviae are pathogens known to cause disease in pigs post-weaning. Due to their fastidious nature, there is increased need for culture-independent diagnostic platforms to detect...
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Seroprevalence of Trichinella sp. in Wild Boars (Sus scrofa) from Yanggu-gun, Gangwon-do, Korea

Lee HJ, Chung OS, Kim JL, Lee SH, Yoo YB, Seo M

A total 7 outbreaks of trichinellosis have occurred in Korea, mostly as a result of consumption of raw wild boar (Sus scrofa) meat. Since only 1 serological survey on wild...
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Pathogenicity of Five Strains of Toxoplasma gondii from Different Animals to Chickens

Wang S, Zhao GW, Wang W, Zhang ZC, Shen B, Hassan IA, Xie Q, Yan RF, Song XK, Xu LX, Li XR

Toxoplasma gondii is a protozoan parasite with a broad range of intermediate hosts. Chickens as important food-producing animals can also serve as intermediate hosts. To date, experimental studies on the...
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Assessing PCV2 antibodies in field pigs vaccinated with different porcine circovirus 2 vaccines using two commercial ELISA systems

Shin MK, Yoon SH, Kim MH, Lyoo YS, Suh SW, Yoo HS

Porcine circovirus type 2 (PCV2) is the primary causative agent for post-weaning, multisystemic, wasting syndrome. Consequently, serologic detection of and vaccination against PCV2 are important for the swine industry. Among...
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Cryptosporidium suis Infection in Post-Weaned and Adult Pigs in Shaanxi Province, Northwestern China

Lin Q, Wang XY, Chen JW, Ding L, Zhao GH

Cryptosporidium spp., ubiquitous enteric parasitic protozoa of vertebrates, recently emerged as an important cause of economic loss and zoonosis. The present study aimed to determine the distribution and species of...
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Induction of antibody and interferon-gamma production in mice immunized with virus-like particles of swine hepatitis E virus

Song YJ, Park WJ, Lee SK, Lee JB, Park SY, Song CS, Lee SW, Seo KH, Kang YS, Song JY, Choi IS

Virus-like particles (VLPs) composed of the truncated capsid protein of swine hepatitis E virus (HEV) were developed and immune responses of mice immunized with the VLPs were evaluated. IgG titers...
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