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Adverse effects of farnesyltransferase inhibitors on insulin actions

Yi SJ, Jhun BH, Kim K

Ras activates a series of downstream effectors, including the mitogen-activated protein kinase pathway and the Rac/Rho pathway after insulin stimulation. Mutations in Ras are found in approximately 30% of all...
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A case study of apple seed and grape allergy with sensitisation to nonspecific lipid transfer protein

Murad A, Katelaris , Baumgart K

Lipid transfer proteins can be an important cause of allergy given their stability and high degree of protein sequence homology. We describe the case of a child who developed two...
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Identification and Molecular Characterization of Parkin in Clonorchis sinensis

Bai X, Kim TI, Lee JY, Dai F, Hong SJ

Clonorchis sinensis habitating in the bile duct of mammals causes clonorchiasis endemic in East Asian countries. Parkin is a RING-between-RING protein and has E3-ubiquitin ligase activity catalyzing ubiquitination and degradation...
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Gene Cloning, Expression and Immunogenicity of the Protective Antigen Subolesin in Dermacentor silvarum

Hu Y, Zeng H, Zhang J, Wang D, Li D, Zhang T, Yang S, Liu J

Subolesin (4D8), the ortholog of insect akirins, is a highly conserved protective antigen and thus has the potential for development of a broad-spectrum vaccine against ticks and mosquitoes. To date,...
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Expression and Characterization of alpha-Methylacyl CoA Racemase from Anisakis simplex Larvae

Kim BJ, Kim SM, Cho MK, Yu HS, Lee YS, Cha HJ, Ock M

Larval excretory-secretory products of Anisakis simplex are known to cause allergic reactions in humans. A cDNA library of A. simplex 3rd-stage larvae (L3) was immunoscreened with polyclonal rabbit serum raised...
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Molecular Cloning and Characterization of a Paramyosin from Clonorchis sinensis

Park TJ, Kang JM, Na BK, Sohn WM

Paramyosin is a myofibrillar protein present in helminth parasites and plays multifunctional roles in host-parasite interactions. In this study, we identified the gene encoding paramyosin of Clonorchis sinensis (CsPmy) and...
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Isolation and identification of a canine coronavirus strain from giant pandas (Ailuropoda melanoleuca)

Gao FS, Hu GX, Xia XZ, Gao YW, Bai YD, Zou XH

Two giant pandas (Ailuropoda melanoleuca) died of unknown causes in a Chinese zoo. The clinical disease profile suggested that the pandas may have suffered a viral infection. Therefore, a series...
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Detection and molecular characterization of infectious bronchitis virus isolated from recent outbreaks in broiler flocks in Thailand

Pohuang T, Chansiripornchai N, Tawatsin A, Sasipreeyajan J

Thirteen field isolates of infectious bronchitis virus (IBV) were isolated from broiler flocks in Thailand between January and June 2008. The 878-bp of the S1 gene covering a hypervariable region...
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Transient phosphorylation of tumor associated microtubule associated protein (TMAP)/cytoskeleton associated protein 2 (CKAP2) at Thr-596 during early phases of mitosis

Hong KU, Choi YB, Lee JH, Kim HJ, Kwon HR, Seong YS, Kim HT, Park J, Bae CD, Hong KM

Tumor associated microtubule associated protein (TMAP), also known as cytoskeleton associated protein 2 (CKAP2) is a mitotic spindle-associated protein whose expression is cell cycle-regulated and also frequently deregulated in cancer...
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Sequence analysis of the S1 glycoprotein gene of infectious bronchitis viruses: identification of a novel phylogenetic group in Korea

Jang JH, Sung HW, Song CS, Kwon HM

Twelve Korean infectious bronchitis viruses (IBVs) were isolated in the field from chickens suspected of being carriers of infectious bronchitis between 2001 and 2003. The S1 glycoprotein genes of these...
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Differentially expressed genes of Acanthamoeba castellanii during encystation

Moon EK, Chung DI, Hong YC, Kong HH

To examine the expressed gene profile during encystation of Acanthamoeba castellanii Castellani, we used differentially expressed gene (DGE) screening by RT-PCR with 20 sets of random primers. From this analysis,...
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Homo-dimerization of RyR1 C-terminus via charged residues in random coils or in an alpha-helix

Lee EH, Allen PD

  • KMID: 1090962
  • Exp Mol Med.
  • 2007 Oct;39(5):594-602.
To investigate the mechanism by which the C-terminus (4,938-5,037) of the ryanodine receptor 1 (RyR1) homo-tetramerizes, forming a functional Ca2+ -release channel, the structural requirements for the tetramerization were studied...
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WNKs: protein kinases with a unique kinase domain

Huang CL, Cha SK, Wang HR, Xie J, Cobb MH

  • KMID: 1090959
  • Exp Mol Med.
  • 2007 Oct;39(5):565-573.
WNKs (with-no-lysine [K]) are a family of serine-threonine protein kinases with an atypical placement of the catalytic lysine relative to all other protein kinases. The roles of WNK kinases in...
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Mutations of CAPN3 in Korean Patients with Limb-Girdle Muscular Dystrophy

Shin JH, Kim HS, Lee CH, Kim CM, Park KH, Kim DS

The limb-girdle muscular dystrophy type 2A (LGMD2A) is a recessively inherited disease caused by a mutation of the calpain 3 gene (CAPN3), and is considered one of the most prevalent...
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Identification of a novel murine organic anion transporter like protein 1 (OATLP1) expressed in the kidney

Jung SM, Lee WK, Kwak JO, Jung SY, Park J, Kim WY, Kim J, Cha SH

  • KMID: 1098082
  • Exp Mol Med.
  • 2006 Oct;38(5):485-493.
The organic anion transporters (OATs) are expressed in various tissues, primarily in the kidney and liver, but they are also expressed in the placenta, small intestine, and the choroid plexus,...
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Genetic Variation in the NSP4 Gene of Human Rotavirus Isolated in Seoul

Cho SL, Ahn JH, Kim K, Chung SI, Lim I, Kim W

The nonstructural protein 4 (NSP4) of rotavirus encoded by gene 10, plays an important role in rotavirus pathogenicity. In this study, NSP4 gene sequences of human rotaviruses circulating in Seoul,...
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Protein kinase A-dependent phosphorylation of B/K protein

Chin H, Choi SH, Jang YS, Cho SM, Kim HS, Lee JH, Jeong SW, Kim IK, Kim GJ, Kwon OJ

  • KMID: 1111407
  • Exp Mol Med.
  • 2006 Apr;38(2):144-152.
We have previously isolated a novel protein "B/K" that contains two C2-like domains. Here, we report the isolatioin and mRNA distribution of a human B/K isoform, and protein kinase A...
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The homotrimeric structure of HtrA2 is indispensable for executing its serine protease activity

Nam MK, Seong YM, Park HJ, Choi JY, Kang S, Rhim H

  • KMID: 1105275
  • Exp Mol Med.
  • 2006 Feb;38(1):36-43.
Serine protease activity of high temperature requrement 2 (HtrA2) is essential for promoting cell death, as well as for protecting against cellular stresses. An X-ray crystallographic study described the formation...
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Cloning and expression of trypsin-encoding cDNA from Blattella germanica and its possibility as an allergen

Ock MS, Kim BJ, Kim SM, Byun KH

In this study, the trypsin gene (bgtryp-1) from the German cockroach, Blattella germanica, was cloned via the immunoscreening of patients with allergies to cockroaches. Nucleotide sequence analysis predicted an 863...
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Overlapping Gene Mutations of Hepatitis B Virus in a Chronic Hepatitis B Patient with Hepatitis B Surface Antigen Loss during Lamivudine Therapy

Lee SY, Choi MS, Lee D, Lee JH, Koh KC, Paik SW, Yoo BC

Disappearance of hepatitis B surface antigens (HBsAg) in chronic hepatitis B usually indicates clearance of hepatitis B virus (HBV) infection. However, false HBsAg negativity with mutations in pre-S2 and 'a'...
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