- Serum Dickkopf-1 as a Biomarker for the Diagnosis of Hepatocellular Carcinoma
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Kim SU, Park JH, Kim HS, Lee JM, Lee HG, Kim H, Choi SH, Baek S, Kim BK, Park JY, Kim DY, Ahn SH, Lee JD, Han KH
- KMID: 2163621
- Yonsei Med J.
- 2015 Sep;56(5):1296-1306.
- doi: 10.3349/ymj.2015.56.5.1296
PURPOSE: Dickkopf-1 (DKK-1) is a Wnt/beta-catenin signaling pathway inhibitor. We investigated whether DKK-1 is related to progression in hepatocellular carcinoma (HCC) cells and HCC patients. MATERIALS AND METHODS: In vitro reverse-transcription... -
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- A Melting Method for RNA Extraction from the Mucosal Membrane of the Mouse Middle Ear
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Seo YJ, Kim SH, Moon IS, Choi JY
- KMID: 2070030
- Yonsei Med J.
- 2015 Mar;56(2):497-502.
- doi: 10.3349/ymj.2015.56.2.497
PURPOSE: There is much confusion surrounding the methods of RNA extraction from the middle ear mucosa of mice. In this study, we worked to develop a "melting method," which is... -
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- Sample type is vital for diagnosing infection with peste des petits ruminants virus by reverse transcription PCR
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Luka PD, Ayebazibwe C, Shamaki D, Mwiine FN, Erume J
- KMID: 1389773
- J Vet Sci.
- 2012 Sep;13(3):323-325.
- doi: 10.4142/jvs.2012.13.3.323
Peste des petits ruminants (PPR) diagnosis from suspected samples from sheep and goats was carried out. Buffy coat, tissues, and oculo-nasal swabs were analyzed using nucleoprotein (NP3/NP4) and fusion protein... -
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- Detection of PML/RARA Rearrangement by Reverse Transcriptase-PCR and Sequencing in a Case of Microgranular Acute Promyelocytic Leukemia Lacking t(15;17) on Karyotype and FISH
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Kim KE, Woo KS, Kim SH, Han JY
- KMID: 1077411
- Korean J Lab Med.
- 2009 Oct;29(5):379-383.
- doi: 10.3343/kjlm.2009.29.5.379
We report a case of morphologically microgranular acute promyelocytic leukemia with PML/RARA fusion transcripts demonstrated by reverse transcriptase-PCR and cDNA sequencing, and no PML/RARA fusion detected by karyotype and FISH... -
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- Evaluation of Seeplex(TM) RV Detection Kit for Detecting Rhinovirus, Human Metapneumovirus, and Coronavirus
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Sung H, Park SJ, Woo YD, Choi BH, Kim MN
- KMID: 854837
- Korean J Lab Med.
- 2008 Apr;28(2):109-117.
- doi: 10.3343/kjlm.2008.28.2.109
BACKGROUND: Direct antigen test (DAT) and culture are primary tests to diagnose infections by respiratory viruses, but are mainly available for the traditional viral pathogens such as respiratory syncytial... -
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- Detection of 12 Respiratory Viruses with Two-set Multiplex Reverse Transcriptase-PCR Assay Using a Dual Priming Oligonucleotide System
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Yoo SJ, Kuak EY, Shin BM
- KMID: 1781546
- Korean J Lab Med.
- 2007 Dec;27(6):420-427.
- doi: 10.3343/kjlm.2007.27.6.420
BACKGROUND: We intended to evaluate the diagnostic usefulness of a multiplex reverse transcriptase- PCR (mRT-PCR) assay kit under dual priming oligonucleotide system (DPO) for the childhood acute respiratory tract... -
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- Comparison of the Urine ThinPrep Test and Melanoma Antigen Gene RT-PCR for the Detection of Bladder Cancer According to Three Urine Collection Methods
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Kim DY, Cho HJ, Oh HK, Shin IH, Jeon CH
- KMID: 1121660
- Korean J Lab Med.
- 2007 Feb;27(1):50-55.
- doi: 10.3343/kjlm.2007.27.1.50
BACKGROUND: For the detection of transitional cell carcinoma (TCC) of the bladder, we compared the sensitivities and specificities between the ThinPrep test and Melanoma Antigen Gene (MAGE) test with... -
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- Development of a Lightcycler-based reverse transcription polymerase chain reaction for the detection of foot-and-mouth disease virus
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Oem JK, Kye SJ, Lee KN, Kim YJ, Park JY, Park JH, Joo YS, Song HJ
- KMID: 1091201
- J Vet Sci.
- 2005 Sep;6(3):207-212.
One step TaqMan real-time reverse transcription polymerase chain reaction (R/T RT-PCR)using a set of primers/probes was developed for the detection of foot-and-mouth disease (FMD)virus. The gene-specific probes labeled fluorogen for... -
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- Quantitative Analysis of SMN1 Gene and Estimation of SMN1 Deletion Carrier Frequency in Korean Population based on Real-Time PCR
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Lee TM, Kim SW, Lee KS, Jin HS, Koo SK, Jo I, Kang S, Jung SC
- KMID: 1778571
- J Korean Med Sci.
- 2004 Dec;19(6):870-873.
- doi: 10.3346/jkms.2004.19.6.870
Spinal muscular atrophy (SMA) is an autosomal recessive disorder, caused by homozygous absence of the survival motor neuron gene (SMN1) in approximately 94% of patients. Since most carriers have only... -
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- Search for Genes Potentially Related to Germ Tube Formation in Candida albicans by Differential-Display Reverse Transcription Polymerase Chain Reaction
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Park JY, Kim DH, Shin WS, Seo HS, Kim KH, Lee KH, Koh CM, Bong JP
- KMID: 1719077
- Yonsei Med J.
- 2003 Feb;44(1):110-118.
- doi: 10.3349/ymj.2003.44.1.110
Candida albicans exhibits the ability to grow in either a yeast or a mycelia form in response to different environmental factors. The mycelia form, found in infected tissues, is important... -
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