- Report on HbA(1c) Proficiency Testing in Asia in 2012
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Umemoto M, Hoshino T, Miyashita , Tani W, Kuwa K
- KMID: 2363209
- Ann Lab Med.
- 2015 May;35(3):352-355.
- doi: 10.3343/alm.2015.35.3.352
In 2010, the Japan Diabetes Society decided to introduce the National Glycohemoglobin Standardization Program (NGSP) values into clinical practice. Accordingly, NGSP Certification of Japanese manufacturers of HbA(1c)-related diagnostic reagents and... -
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- Assessment of the Trueness and Inter-Laboratory Precision of Routine Uric Acid Assays Using 4 Frozen Pooled Serum Samples Measured by the Japan Society of Clinical Chemistry's HPLC Method
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Jiang Y, Liu O, Xu G
- KMID: 1791902
- Ann Lab Med.
- 2014 Mar;34(2):104-110.
- doi: 10.3343/alm.2014.34.2.104
BACKGROUND: Reference procedures are required for evaluating the accuracy of routine analytical systems for uric acid (UA). External quality assessment (EQA) for UA has only been conducted with quality controls... -
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- Working Towards Accreditation by the International Standards Organization 15189 Standard: How to Validate an In-house Developed Method an Example of Lead Determination in Whole Blood by Electrothermal Atomic Absorption Spectrometry
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Garcia Hejl C, Ramirez JM, Vest P, Chianea D, Renard C
- KMID: 1791951
- Ann Lab Med.
- 2014 Sep;34(5):367-371.
- doi: 10.3343/alm.2014.34.5.367
Laboratories working towards accreditation by the International Standards Organization (ISO) 15189 standard are required to demonstrate the validity of their analytical methods. The different guidelines set by various accreditation organizations... -
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- Performance evaluation of the HepB Typer-Entecavir kit for detection of entecavir resistance mutations in chronic hepatitis B
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Ahn SH, Chun JY, Shin SK, Park JY, Yoo W, Hong SP, Kim SO, Han KH
- KMID: 1730886
- Clin Mol Hepatol.
- 2013 Dec;19(4):399-408.
- doi: 10.3350/cmh.2013.19.4.399
BACKGROUND/AIMS: Molecular diagnostic methods have enabled the rapid diagnosis of drug-resistant mutations in hepatitis B virus (HBV) and have reduced both unnecessary therapeutic interventions and medical costs. In this study... -
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- Trueness Assessment for Serum Glucose Measurement Using Commercial Systems through the Preparation of Commutable Reference Materials
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Xia C, Liu O, Wang L, Xu G
- KMID: 1380082
- Ann Lab Med.
- 2012 Jul;32(4):243-249.
- doi: 10.3343/alm.2012.32.4.243
BACKGROUND: Commutable reference materials (RMs) are suitable for end-users for evaluating the metrological traceability of values obtained using routine measurement systems. We assessed the performance of 6 routine measurement systems... -
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- Identification of miR-23a as a novel microRNA normalizer for relative quantification in human uterine cervical tissues
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Shen Y, Li Y, Ye F, Wang F, Wan X, Lu W, Xie X
- KMID: 1107377
- Exp Mol Med.
- 2011 Jun;43(6):358-366.
- doi: 10.3858/emm.2011.43.6.039
Quantitative real-time RT-PCR (RT-qPCR) is being widely used in microRNA expression research. However, few reports detailed a robust identification and validation strategy for suitable reference genes for normalisation in microRNA... -
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- Ultra-Performance Liquid Chromatography-Tandem Mass Spectrometry Measurement of Leukocyte Arylsulfatase A Activity Using a Natural Substrate
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Han M, Jun SH, Song SH, Park HD, Park KU, Song J
- KMID: 2363170
- Ann Lab Med.
- 2015 Jan;35(1):165-168.
- doi: 10.3343/alm.2015.35.1.165
No abstract available. -
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- Investigation of Water Safety in Non-treated Drinking Water with Trace Toxic Metals
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Ly SY, Kim DH, Lee GE
- KMID: 2317837
- Toxicol Res.
- 2013 Sep;29(3):211-215.
The trace toxic metal copper was assayed using mercury immobilized on a carbon nanotube electrode (MCW), with a graphite counter and a reference electrode. In this study, a macro-scale convection... -
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- Effect of local background intensities in the normalization of cDNA microarray data with a skewed expression profiles.
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KIm JH, Shin DM, Lee YS
- KMID: 1380230
- Exp Mol Med.
- 2002 Jul;34(3):224-232.
Normalization of the data of cDNA microarray is an obligatory step during microarray experiments due to the relatively frequent non-specific errors. Generally, normalization of microarray data is based on the... -
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- Suitable reference genes for relative quantification of miRNA expression in prostate cancer
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Schaefer A, Jung M, Miller K, Lein M, Kristiansen G, Erbersdobler A, Jung K
- KMID: 994398
- Exp Mol Med.
- 2010 Nov;42(11):749-758.
- doi: 10.3858/emm.2010.42.11.076
Real time quantitative PCR (qPCR) is the method of choice for miRNA expression studies. For relative quantification of miRNAs, normalization to proper reference genes is mandatory. Currently, no validated reference... -
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- A New Method of Measuring the Amount of Soft Tissue in Pulmonary Ground-Glass Opacity Nodules: a Phantom Study
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Lee KW, Im JG, Kim TJ, Dae CM
- KMID: 1758455
- Korean J Radiol.
- 2008 Jun;9(3):219-225.
- doi: 10.3348/kjr.2008.9.3.219
OBJECTIVE: To devise a new method to measure the amount of soft tissue in pulmonary ground-glass opacity nodules, and to compare the use of this method with a previous volumetric... -
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- Diagnostic Performance of Body Mass Index Using the Western Pacific Regional Office of World Health Organization Reference Standards for Body Fat Percentage
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Yoon JL, Cho JJ, Park KM, Noh HM, Park YS
- KMID: 2129646
- J Korean Med Sci.
- 2015 Feb;30(2):162-166.
- doi: 10.3346/jkms.2015.30.2.162
Associations between body mass index (BMI), body fat percentage (BF%), and health risks differ between Asian and European populations. BMI is commonly used to diagnose obesity; however, its accuracy in... -
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- Liquid Chromatography-Mass Spectrometry-Based In Vitro Metabolic Profiling Reveals Altered Enzyme Expressions in Eicosanoid Metabolism
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Lee SH, Kim EJ, Lee DH, Lee WY, Chung BC, Seo HS, Choi MH
- KMID: 2373554
- Ann Lab Med.
- 2016 Jul;36(4):342-352.
- doi: 10.3343/alm.2016.36.4.342
BACKGROUND: Eicosanoids are metabolites of arachidonic acid that are rapidly biosynthesized and degraded during inflammation, and their metabolic changes reveal altered enzyme expression following drug treatment. We developed an eicosanoid... -
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- Optimization of Allergen Standardization
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Jeong KY, Hong CS, Lee JS, Park JW
- KMID: 1777010
- Yonsei Med J.
- 2011 May;52(3):393-400.
- doi: 10.3349/ymj.2011.52.3.393
Preparation of high quality allergen extracts is essential for the diagnosis and immunotherapy of allergic disorders. Standardization of allergen extracts concerns determination of the allergen unit, development of reference material... -
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- Validity of Glycated Hemoglobin in Screening and Diagnosing Type 2 Diabetes Mellitus in Chinese Subjects
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Yu Y, Ouyang XJ, Lou QL, Gu LB, Mo YZ, Ko GT, Chow CC, So WY, Ma R, Kong A, Brown N, Nan J, Chan J, Bian RW
- KMID: 1776564
- Korean J Intern Med.
- 2012 Mar;27(1):41-46.
- doi: 10.3904/kjim.2012.27.1.41
BACKGROUND/AIMS: The application of glycated hemoglobin (HbA1c) for the diagnosis of diabetes is currently under extensive discussion. In this study, we explored the validity of using HbA1c as a screening... -
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- Enzymatic method for assaying calcium in serum with Ca++-ATPase
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Javed HM, Michelangeli F
- KMID: 1088653
- Exp Mol Med.
- 2003 Feb;35(1):17-22.
A kinetic assay for total calcium in serum was developed which is based on the activation of Ca++-ATPase by free Ca++ [Ca++]f maintained by EGTA in the reaction mixture. The... -
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- Quantification of Human Plasma-Busulfan Concentration by Liquid Chromatography-Tandem Mass Spectrometry
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Moon SY, Lim MK, Hong S, Jeon Y, Han M, Song SH, Lim KS, Yu KS, Jang IJ, Lee JW, Kang HJ, Song J
- KMID: 1781353
- Ann Lab Med.
- 2014 Jan;34(1):7-14.
- doi: 10.3343/alm.2014.34.1.7
BACKGROUND: Busulfan, an alkylating agent administered prior to hematopoietic stem cell transplantation, has a narrow therapeutic range and wide variability in metabolism. We developed a liquid chromatography-tandem mass spectrometry (LC-MS/MS)... -
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- Analysis of Acid Sphingomyelinase Activity in Dried Blood Spots Using Tandem Mass Spectrometry
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Legnini E, Orsini JJ, Muhl A, Johnson B, Dajnoki A, Bodamer OA
- KMID: 1387345
- Ann Lab Med.
- 2012 Sep;32(5):319-323.
- doi: 10.3343/alm.2012.32.5.319
BACKGROUND: Niemann Pick disease (NP) is a rare, lysosomal storage disorder due to deficiency of the intra-lysosomal enzyme acid sphingomyelinase (ASM) resulting in intracellular accumulation of sphingomyelin. We evaluated a... -
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- Standard versus Rotation Technique for Insertion of Supraglottic Airway Devices: Systematic Review and Meta-Analysis
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Park JH, Lee JS, Nam SB, Ju JW, Kim MS
- KMID: 2374134
- Yonsei Med J.
- 2016 Jul;57(4):987-997.
- doi: 10.3349/ymj.2016.57.4.987
PURPOSE: Supraglottic airway devices have been widely utilized as an alternative to tracheal intubation in various clinical situations. The rotation technique has been proposed to improve the insertion success rate... -
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- Internal Amplification Control for a Cryptosporidium Diagnostic PCR: Construction and Clinical Evaluation
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Hawash Y, Ghonaim MM, Al-Hazmi A
- KMID: 2381810
- Korean J Parasitol.
- 2015 Apr;53(2):147-154.
- doi: 10.3347/kjp.2015.53.2.147
Various constituents in clinical specimens, particularly feces, can inhibit the PCR assay and lead to false-negative results. To ensure that negative results of a diagnostic PCR assay are true, it... -
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