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Association Between Toxin-antitoxin Systems on Plasmids and Persister Formation in CTX-15-producing Klebsiella pneumoniae ST11 Isolates

Chung ES, Kim SY, Ko KS

We investigated the effect of toxin-antitoxin (TA) systems in bla(CTX-M-15)-bearing plasmids of Klebsiella pneumoniae on persister formation. The persister formation rate was notably high in transconjugants in plasmids bearing TA...
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Detection of mcr-1 Plasmids in Enterobacteriaceae Isolates From Human Specimens: Comparison With Those in Escherichia coli Isolates From Livestock in Korea

Yoon EJ, Hong JS, Yang JW, Lee KJ, Lee H, Jeong SH

BACKGROUND: The emerging mobile colistin resistance gene, mcr-1, is an ongoing worldwide concern and an evaluation of clinical isolates harboring this gene is required in Korea. We investigated mcr-1-possessing Enterobacteriaceae...
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Extensively Drug-Resistant Escherichia coli Sequence Type 1642 Carrying an IncX3 Plasmid Containing the blaKPC-2 Gene Associated with Transposon Tn4401a

Jeong S, Kim JO, Yoon EJ, Bae IK, Lee W, Lee H, Park Y, Lee K, Jeong SH

BACKGROUND: Extensively drug-resistant (XDR) Enterobacteriaceae carrying the bla(KPC) gene have emerged as a major global therapeutic concern. The purpose of this study was to analyze the complete sequences of plasmids...
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Profiling of antimicrobial resistance and plasmid replicon types in beta-lactamase producing Escherichia coli isolated from Korean beef cattle

Shin SW, Jung M, Shin MK, Yoo HS

In this study, 78 isolates of Escherichia coli isolated from Korean beef cattle farms were investigated for the production of extended-spectrum beta-lactamase (ESBL) and/or AmpC beta-lactamase. In the disc diffusion...
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Immunogenicity and efficacy of a plasmid DNA rabies vaccine incorporating Myd88 as a genetic adjuvant

Ullas PT, Desai A, Madhusudana SN

PURPOSE: Myeloid differentiation factor 88 (Myd88), a ubiquitous Toll-like receptor adaptor molecule, has been reported to play important roles in B cell responses to infections and vaccination. The present study...
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Comparison of Minicircle with Conventional Plasmid for the Non-viral Vascular Endothelial Growth Factor (VEGF) Gene Therapy

Kwon M, Lee S, Chung H, Yoon C, Kim M, Park J

BACKGROUND: Delayed wound healings in diabetic patients are related with the impairment of the expressions of various growth factors. Treatments using growth factors have been attempted on diabetic foot ulcer....
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Cryptic Plasmid Amplification of Chlamydia trachomatis at a Korean Health Center for Female Commercial Sex Workers

Lee G, Sohng I

PURPOSE: Chlamydia trachomatis infection is the most common bacterial sexually transmitted disease. It is generally accepted that female commercial sex workers (FCSWs) are at an increased risk of incurring sexually...
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Highly Efficient Gene Expression in Rabbit Synoviocytes Using EBV-Based Plasmid

Kim JY, Oh ST, Youn J, Lee SK

BACKGROUND: Rheumatoid arthritis (RA) is an autoimmune disorder characterized by chronic synovial inflammation which leads to joint destruction. Gene therapy of RA targets the players of inflammation or articular destruction....
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Changes of Transfection Efficiencies of Plasmid LacZ Genes in Human Gynecologic Cancer Cell Lines

Kim BH, Bae SM, Han YJ, Lee HS, Jo YS, Lee KH, Hur SY, Kim JH, Kim YW, Ro DY, Lee JM, Namkoong S, Kim DJ, Kim CK, Ahn WS

  • KMID: 2272332
  • Korean J Obstet Gynecol.
  • 2004 May;47(5):870-879.
OBJECTIVE: The transfection efficiencies of gynecologic cancer cell lines were investigated by different mediated transfection methods using recombinant LacZ plasmid (pRcCMVLacZ and pAAVCMVLacZ). METHODS: In this study, the gynecologic cancer cell...
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Construction of A Stable Full-Length cDNA Clone of Japanese Encephalitis Virus Strain SA14-14-2 Using Low Copy Number Plasmid

Min KI, Kim YM, Choo MS, Baek SY, Kim JO, Ryu SR, Min BS, Kim Y, Park MK, Byeon WH, Hur SJ

  • KMID: 1579128
  • J Bacteriol Virol.
  • 2004 Dec;34(4):339-353.
Recently the reverse genetics system contributed to the progresses in the investigation of positive-stranded RNA viruses. Here, we report the successful construction of a stable full-length infectious cDNA clone of...
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Construction of Various Copy Number Plasmid Vectors and Their Utility for Genome Sequencing

Yang TJ, Yu Y, Frisch DA, Lee S, Kim HR, Kwon SJ, Park BS, Wing RA

  • KMID: 1515374
  • Genomics Inform.
  • 2004 Dec;2(4):174-179.
We developed various plasmid cloning vectors that are useful in the construction of genomic and shotgun libraries. Two medium copy vectors, pCUGIblu21(pCb21) and pAGIblu21 (pAb21), which are resistant to kanamycin...
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Characterization of Resistance Plasmid of Shigella sonnei Isolates from the Outbreak at Cheju Island in 2000

Pai H, Kim JD, Cheon SM, Chang SJ, Lim YH, Kim YK, Lee BK, Kim S

  • KMID: 2285105
  • Infect Chemother.
  • 2004 Oct;36(5):259-264.
A large outbreak of Shigella sonnei gastrointestinal infections occurred at Cheju Island in Korea from May to August 2000. We selected 54 strains which were isolated from the primary treatment...
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A Small Cryptic Plasmid pZMO1 of Zymomonas mobilis ATCC10988

Kang HL, Kang HS

  • KMID: 1973996
  • Genomics Inform.
  • 2003 Sep;1(1):55-60.
The nucleotide sequence of pZMO1, a small cryptic plasmid of Zymomonas mobilis ATCC10988 was determined. Analysis of 1,680 bp of sequence revealed 69% identity with Shigella sonnei plasmid, pKYM and...
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Site-Specific Mutagenesis in Escherichia coli by Bulky Exocyclic Amino-Substituted Guanine and Adenine Derivatives in Double-Stranded or Gapped Plasmids

Moon KY

  • KMID: 1807225
  • Cancer Res Treat.
  • 2003 Feb;35(1):75-80.
PURPOSE: 7-Bromomethylbenz[alpha]anthracene is a known mutagen and carcinogen. The mutagenic potency of its two major DNA adducts, i.e., N2-(benz[alpha]anthracen-7-ylmethyl)-2'-deoxyguanosine (b[alpha]a2G) and N6-(benz[alpha]anthracen-7-ylmethyl)-2'-deoxyadenosine (b[alpha]a6A), as well as the simpler benzylated analogs,...
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Genetic Diversity among Virulent Mega Plasmids pXO1 and pXO2 of Bacillus anthracis Isolated in Korea

Mun SH, Yoo CK, Oh HB, Seong WK, Chun JH, Yu JY, Lee SS

  • KMID: 2054968
  • J Bacteriol Virol.
  • 2003 Dec;33(4):253-264.
We compared genetic variations in virulence mega plasmids pXO1 and pXO2 of twenty-seven Bacillus anthracis strains from Korean patients and environmental samples together with those of Bacillus anthracis Sterne, Pasteur...
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A Molecular Epidemiologic Study by Plasmid DNA and Random Amplified Polymorphic DNA Analyses of Salmonella enterica serotype Enteritidis Isolated in Food Borne Outbreaks in Gwangju, 1997

Yang SC, Bae M, Kim SH, Chung JK, Ha DR, Won YJ, Chung SS, Ryu PY

  • KMID: 2170072
  • Infect Chemother.
  • 2003 Oct;35(5):289-297.
BACKGROUND: During the past 10 years, there has been an increased incidence of gastrointestinal infections caused by salmonellae in Korea. In 1999, there were several outbreaks and sporadic occurrences of...
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Insulin Gene Therapy Using HVJ-liposome and Epstein-Barr Virus Plasmid in Murine Streptozotocin Induced Diabetes

Kim YD, Park KG, Han SW, Ahn JD, Lee HJ, Kim MJ, Kim HS, Park NH, Lee IK

  • KMID: 1515209
  • J Korean Diabetes Assoc.
  • 2003 Oct;27(5):405-413.
BACKGROUND: Despite improvement in insulin preparation and delivery, the use of insulin therapy alone to maintain normal glucose concentration and prevent the development of diabetic complication is not easy. Therefore,...
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Use of R plasmid and bla Gene for Epidemiological Fingerprinting of Clinical Isolates of Enterobacteriaceae resistant to beta- lactam Antibiotics

Seol SY, Won JH, Kim NH, Yu HS, Lee YC, Cho DT, Kim JW

  • KMID: 1571504
  • J Bacteriol Virol.
  • 2001 Sep;31(3):217-227.
Clinical isolates of Enterobacteriaceae (189 Klebsiella, 61 Enterobacter, 32 Serratia, 19 E. coli, 7 Proteus, and 3 Citrobacter) from one university hospital were epidemiologically analyzed by using transferable R plasmids...
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Characteristics of gene transfer and VEGF expression using naked plasmid vectors in the rat heart

Jeong JO, Park SJ, Huh JE, Jung EA, Oh JH, Gwon HC, Lee YJ, Kim S, Park JE, Lee WR, Jeon ES, Kim DK

  • KMID: 1600596
  • Korean J Med.
  • 2001 Jan;60(1):3-15.
BACKGROUND: The purpose of this study was to compare gene expression among newly designed eukaryotic expression vectors, and to characterize the pattern of vascular endothelial growth factor(VEGF) expression using the...
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IL-10 Plasmid DNA Delivery in NOD Mice for the Prevention of Autoimmune Pancreatic Beta Cell Destruction

Koh JJ, Ko KS, Park JS, Kim WB, Park KS, Kim SY, Lee HK, Shin SG, Kim SW

  • KMID: 2199619
  • J Korean Soc Endocrinol.
  • 2000 Jun;15(2):262-271.
BACKGROUND: Recently, we have reported that biodegradable poly [-(4-aminobutyl)-L-glycolic acid] (PAGA) can condense and protect plasmid DNA from DNase I. In this study, we investigated whether the systemic administration of...
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