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Various macromolecules in in vitro growth medium influence growth, maturation, and parthenogenetic development of pig oocytes derived from small antral follicles

Lee H, Lee Y, Lee J, Lee GS, Lee ST, Lee E

This study was performed to examine the effects of various macromolecules in in vitro growth (IVG) media on the growth, maturation, and parthenogenesis (PA) of pig oocytes derived from small...
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A survey on the awareness and knowledge about elective oocyte cryopreservation among unmarried women of reproductive age visiting a private fertility center

Hong YH, Park JW, Kim H, Kim SK, Choo CW, Jee BC, Suh CS, Kim SH

OBJECTIVE: The aim of this study was to investigate the level of awareness and knowledge regarding elective oocyte cryopreservation (OC) among unmarried women of reproductive age in Korea. METHODS: A survey...
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Effects of Paroxetine on a Human Ether-a-go-go-related Gene (hERG) K⁺ Channel Expressed in Xenopus Oocytes and on Cardiac Action Potential

Hong HK, Hwang S, Jo SH

K⁺ channels are key components of the primary and secondary basolateral Cl- pump systems, which are important for secretion from the salivary glands. Paroxetine is a selective serotonin reuptake inhibitor...
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Developmental competence of in vitro-matured human oocytes obtained from pregnant and non-pregnant women

Pongsuthirak P, Vutyavanich T

OBJECTIVE: The aim of this study was to compare the rate of maturation, fertilization, and embryo development of in vitro-matured human oocytes derived from pregnant and non-pregnant women. METHODS: Immature oocytes...
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Evaluation of human embryo development in in vitro fertilization- and intracytoplasmic sperm injection-fertilized oocytes: A time-lapse study

Kim HJ, Yoon HJ, Jang JM, Lee WD, Yoon SH, Lim JH

OBJECTIVE: We investigated whether the insemination method (in vitro fertilization [IVF] or intracytoplasmic sperm injection [ICSI]) affected morphokinetic events and abnormal cleavage events in embryonic development. METHODS: A total of 1,830...
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The effects of different types of media on in vitro maturation outcomes of human germinal vesicle oocytes retrieved in intracytoplasmic sperm injection cycles

Fesahat F, Dehghani Firouzabadi R, Faramarzi , Khalili MA

OBJECTIVE: Optimizing in vitro maturation (IVM) media to achieve better outcomes has been a matter of interest in recent years. The aim of this prospective clinical trial was to investigate...
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Oocyte maturation under a biophoton generator improves preimplantation development of pig embryos derived by parthenogenesis and somatic cell nuclear transfer

Lee J, Shin H, Lee W, Lee ST, Lee GS, Hyun SH, Lee E

This study was conducted to determine the effects of biophoton treatment during in vitro maturation (IVM) and/or in vitro culture (IVC) on oocyte maturation and embryonic development in pigs. An...
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Effects of sperm insemination on the final meiotic maturation of mouse oocytes arrested at metaphase I after in vitro maturation

Yoon J, Juhn KM, Yoon SH, Ko Y, Lim JH

OBJECTIVE: The aims of this study were to investigate whether fertilization could induce the resumption of meiosis in mouse oocytes arrested at metaphase I (MI) after in vitro maturation (IVM),...
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Relationship between meiotic spindles visualization and intracytoplasmic sperm injection outcomes in human oocytes

Asa E, Tabatabaee R, Farrokhi A, Nejatbakhsh R

In assisted reproductive techniques, the operator attempts to select morphologically best embryos to predict embryo viability. Development of polarized light microscope, which evaluates the oocytes' spindles according to birefringence of...
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Acceptance of Oocytes Freezing for Fertility Preservation for Social Reasons Among Unmarried Korean Women

Cho HS, Kim HO

PURPOSE: To evaluate the awareness of unmarried Korean women about the relationship between fertility and age with respect to the need to consider oocyte freezing to preserve fertility in cases...
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Embryonic development after exposure of mouse oocyte to various amount of ovarian endometriotic fluid

Kim H, Jeong M, Kim SK, Jee BC

This study assesses the fertilization and blastocyst-forming rate in mice cumulus-oocyte complexes (COCs) after the exposure of human ovarian endometriotic fluid. Endometriotic fluid was obtained from a single patient by...
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Cryopreservation of in vitro matured oocytes after ex vivo oocyte retrieval from gynecologic cancer patients undergoing radical surgery

Park CW, Lee SH, Yang KM, Lee IH, Lim KT, Lee KH, Kim TJ

OBJECTIVE: The aim of this study was to report a case series of in vitro matured (IVM) oocyte freezing in gynecologic cancer patients undergoing radical surgery under time constraints as...
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Nomogram to predict the number of oocytes retrieved in controlled ovarian stimulation

Moon KY, Kim H, Lee JY, Lee JR, Jee BC, Suh CS, Kim KC, Lee WD, Lim JH, Kim SH

OBJECTIVE: Ovarian reserve tests are commonly used to predict ovarian response in infertile patients undergoing ovarian stimulation. Although serum markers such as basal follicle-stimulating hormone (FSH) or random anti-Müllerian hormone...
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Epigenetic modification of long interspersed elements-1 in cumulus cells of mature and immature oocytes from patients with polycystic ovary syndrome

Pruksananonda K, Wasinarom , Sereepapong W, Sirayapiwat P, Rattanatanyong P, Mutirangura A

OBJECTIVE: The long interspersed elements (LINE-1, L1s) are a group of genetic elements found in large numbers in the human genome that can translate into phenotype by controlling genes. Growing...
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Vitrification, in vitro fertilization, and development of Atg7 deficient mouse oocytes

Bang S, Lee GK, Shin H, Suh CS, Lim HJ

OBJECTIVE: Autophagy contributes to the clearance and recycling of macromolecules and organelles in response to stress. We previously reported that vitrified mouse oocytes show acute increases in autophagy during warming....
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Pregnancy and fertilization potential of immature oocytes retrieved in intracytoplasmic sperm injection cycles

Ko DS, Lee SH, Park DW, Yang KM, Lim CK

OBJECTIVE: The goal of this study was to evaluate the pregnancy potential of immature (metaphase I or germinal vesicle stage) oocytes retrieved in intracytoplasmic sperm injection (ICSI) cycles. METHODS: A total...
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The potential role of granulosa cells in the maturation rate of immature human oocytes and embryo development: A co-culture study

Jahromi BN, Mosallanezhad Z, Matloob N, Davari M, Ghobadifar MA

OBJECTIVE: In order to increase the number of mature oocytes usable for intracytoplasmic sperm injection (ICSI), we aimed to investigate the effect of co-culturing granulosa cells (GCs) on human oocyte...
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Artificial oocyte activation in intracytoplasmic sperm injection cycles using testicular sperm in human in vitro fertilization

Kang HJ, Lee SH, Park YS, Lim CK, Ko DS, Yang KM, Park DW

OBJECTIVE: Artificial oocyte activation (AOA) is an effective method to avoid total fertilization failure in human in vitro fertilization-embryo transfer (IVF-ET) cycles. AOA performed using a calcium ionophore can induce...
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Embryonic development in human oocytes fertilized by split insemination

Kim MS, Kim J, Youm HW, Park JY, Choi HY, Jee BC

OBJECTIVE: To compare the laboratory outcomes of intracytoplasmic sperm injection (ICSI) and conventional insemination using sibling oocytes in poor prognosis IVF cycles where ICSI is not indicated. METHODS: Couples undergoing IVF...
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Fertilization and embryo quality of mature oocytes with specific morphological abnormalities

Yu EJ, Ahn H, Lee JM, Jee BC, Kim SH

OBJECTIVE: To investigate fertilization and embryo quality of dysmorphic mature oocytes with specific morphological abnormalities obtained from intracytoplasmic sperm injection (ICSI). METHODS: The fertilization rate (FR) and embryo quality were compared...
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