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Effect of temperature and medium on the viability of Mycobacterium leprae during long term-storage

Park JH, Kim YJ, Kim JP

BACKGROUND: Mycobacterium leprae (M. leprae) is pathogenic bacterium with polymorphic, acid-fast properties and causes leprosy that it is called Hansen's disease. Leprosy can be completely cured using multidrug therapy (MDT),...
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Comparison of Effective DNA Extraction method for Molecular biological study of Mycobacterium leprae

Kim YJ, Park JH, Kim JP

BACKGROUND: Leprosy is an important health problem in many geographical areas yet. It is caused through a cough or contact with fluid from the nose of a person infected by...
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Identification of Mycobacterium leprae and Mycobacterium lepromatosis in Formalin-Fixed and Paraffin-Embedded Skin Samples from Mexico

Torres-Guerrero E, Sánchez-Moreno EC, Atoche-Diéguez CE, Carrillo-Casas EM, Arenas R, Xicohtencatl-Cortes J, Hernández-Castro R

BACKGROUND: The causative agents of leprosy are the well-known Mycobacterium leprae and the newly discovered Mycobacterium lepromatosis. This agent was found in 2008, and it was found to be the...
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Molecular Epidemiology of Mycobacterium leprae as Determined by Structure-Neighbor Clustering in Korea found cases

Kim JP

BACKGROUND: It has proven challenging to investigate the molecular epidemiology of Mycobacterium leprae, the causative agent of leprosy, due to difficulties with culturing of the organism and a lack of...
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Evaluation of Propidium Monoazide Real-Time PCR for Viablity of Mycobacterium leprae

Kim JP, Kim YS, Kim CW

BACKGROUND: Conventional acid-fast bacilli (AFB) staining cannot differentiate viable from dead cells. Propidium monoazide (PMA) is a photoreactive DNA-binding dye that inhibits PCR amplification by DNA modification. OBJECT: The author evaluated...
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Expression of Nucleotide-oligomerization Domain (NOD) and Related Genes in Mouse Tissues Infected with Mycobacterium leprae

Kim TH, Chae GT, Kang TJ

The nucleotide-oligomerization domain (NOD) is an important molecule involved in host defense against bacterial infection. To study the role of NODs in the host response to Mycobacterium leprae, we measured...
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Acridine orange stain of Mycobacterium leprae

Kim JP, Lee MS, Kang JW

  • KMID: 2308473
  • Korean Lepr Bull.
  • 2013 Dec;46(1):3-16.
The Ziehl-Neelson's AFB staining method was mainly used for the AFB observation of the diagnosis of leprosy. However, the fluorescent stain performs better and allows the detection of more positive...
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Rapid detection of Mycobacterium leprae for the diagnosis of leprosy: comparison between loop-mediated isothermal amplification and PCR.

Kim JP, Ko YH

  • KMID: 1598599
  • Korean Lepr Bull.
  • 2005 Jun;38(1):25-34.
Loop-mediated isothermal amplification (LAMP) is a novel nucleic acid amplification method that amplifies DNA with high specificity, efficiency, and speed under isothermal conditions. To evaluate the usefulness of LAMP for...
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Prevalence of IgM antibodies to phenolic glycolipid I of Mycobacterium leprae among controls, workers and foreigners in Korea

Kim JP, Kim YS, Cho SN, Ko YH

  • KMID: 1910888
  • Korean Lepr Bull.
  • 2004 Dec;37(2):19-27.
Phenolic glycolipid I (PGL-I) is a Mycobacterium leprae-specific antigen, and the antibodies to the antigen may suggest an M. leprae infection. To compare the M. leprae infection among the populations,...
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Mutations in folP1, rpoB, and gyrB associated with multiple drug resistance in Mycobacterium leprae isolates from South Korea

Lee SB, You EY, Kang TJ, Kim SK, Kim JP, Ko YH, Chae GT

  • KMID: 1516978
  • Korean Lepr Bull.
  • 2003 Jul;36(1):3-9.
The emergence of multiple drug resistant Mycobacterium leprae has emphasized the need for early decision of effective antileprosy drug in the treatment for leprosy patients. Mutations in the genes associated...
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The association study on infection of Mycobacterium leprae and RIPK2

Kim BC, Kang TJ, Chae GT

  • KMID: 1921046
  • Korean Lepr Bull.
  • 2003 Dec;36(2):23-29.
Receptor-interacting serine/threonine kinase 2(RIPK2) is an adaptor molecule involved in the signal pathway of TLRs. However, there is no report on association between RIPK2 expression and infectious disease including...
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Drug resistance in Mycobacterium leprae

Kashivabara Y

  • KMID: 1883232
  • Korean Lepr Bull.
  • 1999 Dec;32(2):1-4.
No abstract available.
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Detection of Mycobacterium leprae in Tissue and Blood by Polymerase Chain Reaction

Lee KS, Oh KY, Ryoo YW, Suh MH, Song JY

BACKGROUND: Methods to detect and quanitify Mycobacterium leprae(M. leprae)are needed for studies involving the epidemiology, pathogenesis, and chemotherapy of leprosy. Serological assays and skin tests lack the sensitivity and specificity...
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Detection of Mycobacterium leprae DNA Using Nested - PCR from Paraffin - embedded Tissue Samples

Park K, Won YH, Kim YP, Chun IK

  • KMID: 1681221
  • Korean J Dermatol.
  • 1994 Jun;32(3):462-468.
BACKGROUND: Diagnosis of paucibacillary leprosy is difficult owing to lack of sensitive diagnostic tools. Recently, several investigators have studied the use of polymerase chain reaction(PCR) to detect Mycobacterium leprae. Using...
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Detection of Mycobacterium leprae in Skin Biopsy Sepcimens From Leprosy patients by Polymerase Chain Reaction

Yoon KH, Cho SN, Lee JB, Kim JD

  • KMID: 1681207
  • Korean J Dermatol.
  • 1994 Jun;32(3):409-415.
BACKGROUND: Polymerase chain reaction(PCR) has brought an oppotunity for rapid detection of Mycobacterium leprae in clinical pecimens for the diagnosis of leprosy. Th DNA segment specific to M. leprae was...
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Effects of Mycobacterium leprae , M . leprae Phenolic Glycolipid - 1 and Cytokines on the Nitric Oxide Generation of RAW 264 . 7 Macrophages

Park SD, Lee JS, Lee BS, Jun CD, Chung HT, Kim JG

  • KMID: 2230814
  • Korean J Dermatol.
  • 1994 Apr;32(2):234-244.
Background: Nitric oxide(NO) has been reproted to play an important role in macrophage-mediated microbicidal capacity for a variety of intracellular pathogens. NO generation is used as an indicator of microbicidal...
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A Case of Histoid Leproma with Mycobacterium leprae Detected in the Peripheral Blood by polymerase Chain Reaction

Oh KY, Lee KS, Song JY

  • KMID: 2230860
  • Korean J Dermatol.
  • 1994 Feb;32(1):167-171.
Histoid leproma usually occur in pat.ients with lepromatous leproy who have had relapsed after being undcr treatment(usually sulfones) for a long time and who have shown variable improvement. A 56...
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Seroactivities to Phenolic Glycolipid I of Mycobacterium leprae Among Leprosy Patients in National sorokdo Hospital and Their Children

Hwang KK, Cho SN

  • KMID: 2038963
  • Korean J Dermatol.
  • 1988 Aug;26(4):513-517.
Using ELlSA, 83 sera from leprosy patients in National Sorokdo Hospital who are clessified bacteriologically positive and 40 sera from patient's children were tested for a.ntibedy to phenolic glycolipid I...
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The Singnificance of Selection of the Finger and the Great Toe in Slit - skin Smears for Mycobacterium Leprae

Sohn JK, Kim SW, Ha YM

  • KMID: 1678062
  • Korean J Dermatol.
  • 1980 Aug;18(4):277-280.
Bacteriological index(BI) was calculated by slit-skin smears taken from the earlobe and the dorsal surfaces of the proximal phalanges of the index finger and of the great toe in 110...
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