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Development of a multiplex loop-mediated isothermal amplification assay to detect shiga toxin-producing Escherichia coli in cattle

Dong HJ, Cho AR, Hahn TW, Cho S

A multiplex loop-mediated isothermal amplification (mLAMP) assay was developed for simultaneous detection of the stx1 and stx2 genes and applied for detection of shiga toxin-producing Escherichia coli (STEC) in cattle...
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Computed tomographic evaluation of cervical vertebral canal and spinal cord morphometry in normal dogs

Seo E, Choi J, Choi M, Yoon J

The height, width, and cross-sectional area of the vertebral canal and spinal cord along with the area ratio of spinal cord to vertebral canal in the cervical vertebra were evaluated...
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Use of aglepristone for the treatment of P4 induced insulin resistance in dogs

Bigliardi , Bresciani , Callegari , Di Ianni F, Morini G, Parmigiani E, Bianchi E

Insulin resistance (IR) in dogs is suspected when hyperglycemia is present despite administration of insulin doses greater than 1.0 to 1.5 UI/kg. IR is caused by increases in counter regulatory...
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Distribution, quantitative load and characterization of Salmonella associated with swine farms in upper-northern Thailand

Tadee P, Kumpapong , Sinthuya D, Yamsakul P, Chokesajjawatee N, Nuanualsuwan S, Pornsukarom S, Molla BZ, Gebreyes W, Patchanee

This study was conducted to analyze the prevalence and quantitative loads of Salmonella spp. on pig farms in Chiang Mai, Lamphun, Thailand to assess loading levels before slaughtering. The serotype...
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Oxidative response of neutrophils to platelet-activating factor is altered during acute ruminal acidosis induced by oligofructose in heifers

Concha C, Carretta M, Alarcon P, Conejeros , Gallardo D, Hidalgo AI, Tadich N, Caceres DD, Hidalgo MA, Burgos RA

Reactive oxygen species (ROS) production is one of the main mechanisms used to kill microbes during innate immune response. D-lactic acid, which is augmented during acute ruminal acidosis, reduces platelet...
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Accuracy of capillary blood 3-beta-hydroxybutyrate determination for the detection and treatment of canine diabetic ketoacidosis

Bresciani , Pietra M, Corradini , Giunti M, Fracassi F

In human medicine, diagnosis of diabetic ketoacidosis (DKA) is usually based on measurement of capillary 3-beta-hydroxybutyrate (3-HB) with a hand held ketone sensor. This study was conducted to determine if...
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Isolation, in vitro propagation, genetic analysis, and immunogenic characterization of an Ehrlichia canis strain from southeastern Brazil

Alves RN, Rieck SE, Ueira-Vieira C, Labruna MB, Beletti ME

Amplification of the 16S rRNA gene from a blood sample obtained from a dog in southeastern Brazil was used to confirm a naturally acquired Ehrlichia (E.) canis infection. Following isolation...
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Identification of abnormal gene expression in bovine transgenic somatic cell nuclear transfer embryos

Cho J, Kang S, Lee BC

This study was conducted to investigate the expression of three genes related to early embryonic development in bovine transgenic cloned embryos. To accomplish this, development of bovine transgenic somatic cell...
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Effect of superovulation on uterine and serum biochemical parameters and its potential association with transferable embryos in Holstein dairy cows

Rasolomboahanginjatovo HS, Chorfi Y, Dupras R, Mills L, Lefebvre R

The objective of this study was to determine the effects of superovulation (SOV) on serum and uterine biochemical parameters, uterine bacteriology and cytology and number of transferable embryos (TE). Dairy...
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Retrospective surveillance of metabolic parameters affecting reproductive performance of Japanese Black breeding cows

Watanabe U, Takagi M, Yamato O, Otoi T, Okamoto K

This retrospective study was conducted to confirm the relationship between pre- and postpartum metabolic parameters and postpartum reproductive performance and to clarify seasonal characteristics of the metabolic parameters by using...
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Accuracy of sonographic diagnosis of pneumoperitoneum using the enhanced peritoneal stripe sign in beagle dogs

Kim SY, Park KT, Yeon SC, Lee HC

The objective of this study was to evaluate the feasibility and accuracy of estimating the smallest amount of abdominal free gas detectible in a large population of beagles by ultrasonography....
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Evaluation of endoscopically obtained duodenal biopsy samples from cats and dogs in an adapter-modified Ussing chamber

Ruhnke I, DeBiasio J, Suchodolski JS, Newman S, Musch MW, Steiner JM

This study was conducted to evaluate an adapter-modified Ussing chamber for assessment of transport physiology in endoscopically obtained duodenal biopsies from healthy cats and dogs, as well as dogs with...
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Equine hyperimmune serum protects mice against Clostridium difficile spore challenge

Yan W, Shin KS, Wang SJ, Xiang H, Divers T, McDonough , Bowman J, Rowlands A, Akey B, Mohamed H, Chang YF

Clostridium (C.) difficile is a common cause of nosocomial diarrhea in horses. Vancomycin and metronidazole have been used as standard treatments but are only moderately effective, which highlights the need...
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Extent of Mycobacterium bovis infection in dairy cattle herds subject to partial culling as determined by an interferon-gamma assay

Je S, Yeo UC, Song T, Kim KC, Park SY, Kim MJ, Cho SN

The interferon-gamma (IFN-gamma) assay is employed as a complementary diagnostic test for bovine tuberculosis (BTB) in many countries. To simplify this assay, we established a 96-well plate format using the...
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A novel low-cost method for Mycobacterium avium subsp. paratuberculosis DNA extraction from an automated broth culture system for real-time PCR analysis

Salgado M, Verdugo C, Heuer C, Castillo P, Zamorano P

PCR is a highly accurate technique for confirming the presence of Mycobacterium avium subsp. paratuberculosis (Map) in broth culture. In this study, a simple, efficient, and low-cost method of harvesting...
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Comparable bone healing capacity of different bone graft matrices in a rabbit segmental defect model

Kim JM, Kim MH, Kang SS, Kim G, Choi SH

We compared the bone healing capacity of three different demineralized bone matrix (DBM) products applied using different carrier molecules (hyaluronic acid [HA] vs. carboxymethylcellulose [CMC]) or bone compositions (cortical bone...
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Effects of resveratrol on the insulin signaling pathway of obese mice

Hong HJ, Kang W, Kim DG, Lee DH, Lee Y, Han CH

The present study was conducted to investigate the effects of resveratrol on the insulin signaling pathway in the liver of obese mice. To accomplish this, we administered resveratrol to high...
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Production and immunogenicity of chimeric virus-like particles containing the spike glycoprotein of infectious bronchitis virus

Lv L, Li X, Liu G, Li R, Liu Q, Shen H, Wang W, Xue C, Cao Y

Infectious bronchitis virus (IBV) poses a severe threat to the poultry industry and causes heavy economic losses worldwide. Vaccination is the most effective method of preventing infection and controlling the...
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Tissue-specific expression of the NOD-like receptor protein 3 in BALB/c mice

Huang Z, Yu M, Tong S, Jia K, Liu R, Wang H, Li S, Ning Z

Activation of the innate immune system requires recognition of pathogen-associated molecular patterns, such as NOD-like receptors. The NOD-like receptor protein 3 (NLRP3) inflammasome is involved in induction of the pro-inflammatory...
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Recombinant Kluyveromyces lactis expressing highly pathogenic porcine reproductive and respiratory syndrome virus GP5 elicits mucosal and cell-mediated immune responses in mice

Zhao H, Wang Y, Ma Z, Wang Y, Feng WH

Currently, killed-virus and modified-live porcine reproductive and respiratory syndrome virus (PRRSV) vaccines are used to control porcine reproductive and respiratory syndrome. However, both types of vaccines have inherent drawbacks; accordingly,...
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