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Optimization of Growth Medium and Fermentation Conditions for the Production of Laccase3 from Cryphonectria parasitica Using Recombinant Saccharomyces cerevisiae

Jeong YS, So KK, Lee JH, Kim JM, Chun GT, Chun J, Kim DH

Statistical experimental methods were used to optimize the medium for mass production of a novel laccase3 (Lac3) by recombinant Saccharomyces cerevisiae TYEGLAC3-1. The basic medium was composed of glucose, casamino...
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Evaluation of Acute and Chronic Effects of D-Galactose on Memory and Learning in Wistar Rats

Chogtu B, Arivazhahan A, Kunder SK, Tilak A, Sori R, Tripathy A

OBJECTIVE: D-galactose has been commonly used in rodent models to induce accelerated effects of aging, including those on learning, memory, and muscular tone and coordination. This is normally seen on...
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Dendropanax morbifera Léveille extract ameliorates D-galactose-induced memory deficits by decreasing inflammatory responses in the hippocampus

Lee KY, Jung HY, Yoo DY, Kim W, Kim JW, Kwon HJ, Kim DW, Yoon YS, Hwang IK, Choi JH

In the present study, we examined the effects of Dendropanax morbifera Léveille leaf extract (DML) on D-galactose-induced morphological changes in microglia and cytokines, including pro-inflammatory cytokines (interleukin [IL]-1β, IL-6, and...
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Protection of the brain through supplementation with larch arabinogalactan in a rat model of vascular dementia

Lim SH, Lee J

BACKGROUND/OBJECTIVES: Vascular dementia (VaD) caused by reduced blood supply to the brain manifests as white matter lesions accompanying demyelination and glial activation. We previously showed that arabinoxylan consisting of arabinose...
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Inhibition of advanced glycation end product formation by burdock root extract

Lee D, Kim CY

PURPOSE: Diabetic complications are a major concern to manage progression of diabetes. Production of advanced glycation end products (AGEs) due to high blood glucose is one of the mechanisms leading...
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Effects of aluminum on the reduction of neural stem cells, proliferating cells, and differentiating neuroblasts in the dentate gyrus of D-galactose-treated mice via increasing oxidative stress

Nam SM, Kim JW, Yoo DY, Kim W, Jung HY, Choi JH, Hwang IK, Seong JK, Yoon YS

Aluminum (Al) accumulation increases with aging, and long-term exposure to Al is regarded as a risk factor for Alzheimer's disease. In this study, we investigated the effects of Al and/or...
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Ethanol Production from Various Sugars and Cellulosic Biomass by White Rot Fungus Lenzites betulinus

Im KH, Nguyen TK, Choi J, Lee TS

Lenzites betulinus, known as gilled polypore belongs to Basidiomycota was isolated from fruiting body on broadleaf dead trees. It was found that the mycelia of white rot fungus Lenzites betulinus...
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Esculetin, a Coumarin Derivative, Inhibits Aldose Reductase Activity in vitro and Cataractogenesis in Galactose-Fed Rats

Kim CS, Kim J, Lee YM, Sohn E, Kim JS

Naturally occurring coumarin compounds have received substantial attention due to their pharmaceutical effects. Esculetin is a coumarin derivative and a polyphenol compound that is used in a variety of therapeutic...
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Delayed Anaphylaxis to Red Meat Associated With Specific IgE Antibodies to Galactose

Wen L, Zhou J, Yin J, Sun JL, Sun Y, Wu K, Katial R

A novel delayed anaphylactic reaction to red meat, associated with tick bites and IgE antibodies against galactose-alpha-1, 3-galactose (alpha-gal), was reported in 2009 in the US, Australia and Europe. In...
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Effects of Exendin-4 on Male Reproductive Parameters of D-Galactose Induced Aging Mouse Model

Ahangarpour A, Oroojan AA, Heidari H

PURPOSE: The purpose of this study was to evaluate the role of exendin-4 on reproductive alteration in a D-galactose-induced aging mouse model. MATERIALS AND METHODS: In this experimental study, 72 male...
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Kinetin inhibits apoptosis of aging spleen cells induced by D-galactose in rats

Li M, Ouyang W, Wu X, Zheng Y, Wei Y, An L

Kinetin (Kn) is a cytokinin growth factor that exerts several anti-aging and antioxidant effects on cells and organs. To investigate the mechanism underlying apoptotic events in aging cells induced by...
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Annual Report on External Quality Assessment of Biochemical Genetics in Korea (2013)

Lee SY, Biochemical Genetics Subcommittee, The Korean Association of Quality Assurance for Clinical Laboratory

  • KMID: 2200442
  • J Lab Med Qual Assur.
  • 2014 Jun;36(2):64-70.
Two trials of external quality assessment (EQA) of conventional newborn screening tests for phenylketonuria, galactosaemia, congenital adrenal hyperplasia, maple syrup urine disease, homocystinuria, and congenital hypothyroidism, as well as of...
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RBC Enzyme Analysis

Song J

  • KMID: 2049286
  • Clin Pediatr Hematol Oncol.
  • 2013 Apr;20(1):8-12.
Among ~20 RBC enzyme deficiencies causing hereditary hemolytic anemia (HRA), deficiencies involving three RBC enzymes such as glucose-6-phosphatase, pyruvate kinase and pyrimidine 5'-nucleodiase were known to be relatively common. The...
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Permeability factors in nephrotic syndrome and focal segmental glomerulosclerosis

Savin VJ, McCarthy E, Sharma M

  • KMID: 1426330
  • Kidney Res Clin Pract.
  • 2012 Dec;31(4):205-213.
Circulating permeability factors have been identified in the plasma of patients with focal segmental glomerulosclerosis (FSGS). Post-transplant recurrence of proteinuria, improvement of proteinuria after treatment with plasmapheresis, and induction of...
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Novel Compound Heterozygote Mutations of the SLC25A13 Gene in an Infant with Neonatal-onset Type II Citrullinemia Detected by Newborn Mass Screening

Lim HS, Kim H, Kim SS, Kim GH, Yoo HW, Shin YL

Citrin deficiency caused by the SLC25A13 gene mutations is associated with both neonatal-onset type II citrullinemia (CTLN2), also known as neonatal intrahepatic cholestasis caused by citrin deficiency and adult-onset CTLN2....
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Activation of Macrophages by Exopolysaccharide Produced by MK1 Bacterial Strain Isolated from Neungee Mushroom, Sarcodon aspratus

Im SA, Wang W, Lee CK, Lee YN

BACKGROUND: The MK1 strain, a novel bacterial isolate from soft-rotten tissue of the Neungee mushroom, produces copious amounts of exopolysaccharide (EPS) in a dextrose minimal medium. This study examined the...
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A Case of Infantile Hepatic Hemangioendothelioma Incidentally Detected during the Evaluation of Galactosemia

Lim RK, Byun SY, Park SS, Kim YD

  • KMID: 2144479
  • J Korean Soc Neonatol.
  • 2010 May;17(1):136-140.
Galactosemia is a group of inherited enzyme deficiencies characterized by increase in the blood galactose levels. This condition may be associated with deficiencies of galactose-1-phosphate uridyl transferase, galactokinase, or uridine...
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Composition and Partial Structure Characterization of Tremella Polysaccharides

Khondka P

Heteropolysaccharides isolated from liquid cultures of nine Tremella species contained 0.3 to 1.2% protein, 2.7 to 5% ash, 0.9 to 3.4% acetyl groups, 76.5 to 84.2% carbohydrates and trace amounts...
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A Case of Classical Galactosemia caused by Compound Heterozygous Mutations of the GALT Gene

Cheon CK, Cho MS, Ko JM, Kim GH, Yoo HW

  • KMID: 2184458
  • J Genet Med.
  • 2008 Dec;5(2):131-135.
Classical galactosemia is an autosomal recessive disorder of galactose metabolism, caused by a deficiency of the enzyme galactose-1-phosphate uridyltransferase (GALT). Buildup of galactose-1-phosphate is toxic at high levels and can...
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Suitable Conditions for Mycelial Growth of Phellinus spp.

Hur H, Imtiaj A, Lee MW, Lee TS

The fungus Phellinus is a mushroom that is widely used medicinally. The optimal conditions for mycelial growth of 13 strains of the fungus were investigated. Mycelial growth was optimal at...
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