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Role of Rotavirus Enterotoxin NSP4 in the Inflammatory Response in Murine Macrophage RAW 264.7 Cells

Kim SA, Than VT, Kim W

The rotavirus nonstructural glycoprotein, NSP4, has been identified as the first viral enterotoxin capable of inducing diarrhea. To investigate the biological function of NSP4 in the inflammatory process, a cDNA...
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Interactome Analysis Reveals that Heterochromatin Protein 1gamma (HP1gamma) Is Associated with the DNA Damage Response Pathway

Kim H, Choi JD, Kim BG, Kang HC, Lee JS

PURPOSE: Heterochromatin protein 1gamma (HP1gamma) interacts with chromosomes by binding to lysine 9-methylated histone H3 or DNA/RNA. HP1gamma is involved in various biological processes. The purpose of this study is...
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IgE Reactivity of the Dog Lipocalin Allergen Can f 4 and the Development of a Sandwich ELISA for Its Quantification

Rytkonen-Nissinen M, Saarelainen S, Randell J, Hayrinen J, Kalkkinen , Virtanen T

PURPOSE: Divergent results on the IgE reactivity of dog-allergic subjects to Can f 4 have been reported. The aim of this study was to evaluate the significance of Can f...
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Human coagulation factor VIII domain-specific recombinant polypeptide expression

Choi SJ, Jang KJ, Lim JA, Kim HS

BACKGROUND: Hemophilia A is caused by heterogeneous mutations in F8. Coagulation factor VIII (FVIII), the product of F8, is composed of multiple domains designated A1-A2-B-A3-C1-C2. FVIII is known to interact...
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Gene Cloning, Expression and Immunogenicity of the Protective Antigen Subolesin in Dermacentor silvarum

Hu Y, Zeng H, Zhang J, Wang D, Li D, Zhang T, Yang S, Liu J

Subolesin (4D8), the ortholog of insect akirins, is a highly conserved protective antigen and thus has the potential for development of a broad-spectrum vaccine against ticks and mosquitoes. To date,...
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Autologous Immunoglobulin Therapy in Patients With Severe Recalcitrant Atopic Dermatitis: A Preliminary Report

Nahm DH, Cho SM, Kim ME, Kim YJ, Jeon SY

The management of severe recalcitrant atopic dermatitis (AD) is a challenging issue for clinicians and patients. We hypothesized that repeated intramuscular injections of autologous immunoglobulin (autologous immunoglobulin therapy: AIGT) might...
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Synapsin-1 and tau reciprocal O-GlcNAcylation and phosphorylation sites in mouse brain synaptosomes

Kang MJ, Kim C, Jeong H, Cho BK, Ryou AL, Hwang D, Mook-Jung , Yi EC

  • KMID: 2154248
  • Exp Mol Med.
  • 2013 Jun;45(6):e29.
O-linked N-acetylglucosamine (O-GlcNAc) represents a key regulatory post-translational modification (PTM) that is reversible and often reciprocal with phosphorylation of serine and threonine at the same or nearby residues. Although recent...
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Mass Spectrometry Analysis for Nitration of Proteins

Kim KP

Various proteomics and immunological methods including mass spectrometry combined with both liquid and 2-D PAGE, and immunodetection have been employed to identify and characterize nitrated proteins from pathological samples. Nitrosative...
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Immunogenicity and Protective Efficacy of a Dual Subunit Vaccine Against Respiratory Syncytial Virus and Influenza Virus

Park MH, Chang J

Respiratory syncytial virus (RSV) and influenza virus are the most significant pathogens causing respiratory tract diseases. Composite vaccines are useful in reducing the number of vaccination and confer protection against...
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Human Infections with Liver and Minute Intestinal Flukes in Guangxi, China: Analysis by DNA Sequencing, Ultrasonography, and Immunoaffinity Chromatography

Jeon HK, Lee D, Park H, Min DY, Rim HJ, Zhang H, Yang Y, Li X, Eom KS

  • KMID: 1792138
  • Korean J Parasitol.
  • 2012 Dec;50(4):391-394.
The prevalence of liver and intestinal fluke infections was determined by surveying inhabitants of Hengxuan, Fusui, and Shanglin villages which were known to be endemic for liver flukes in Guangxi,...
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Development and Clinical Evaluation of a Rapid Serodiagnostic Test for Toxoplasmosis of Cats Using Recombinant SAG1 Antigen

Chong CK, Jeong W, Kim HY, An DJ, Jeoung HY, Ryu JE, Ko AR, Kim YJ, Hong SJ, Yang Z, Nam HW

Rapid serodiagnostic methods for Toxoplasma gondii infection in cats are urgently needed for effective control of transmission routes toward human infections. In this work, 4 recombinant T. gondii antigens (SAG1,...
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Evaluation of glycoproteins purified from adult and larval camel ticks (Hyalomma dromedarii) as a candidate vaccine

El Hakim A, Shahein YE, Abdel-Shafy S, Abouelella AMK, Hamed RR

In order to identify antigens that can help prevent camel tick infestations, three major glycoproteins (GLPs) about 97, 66 and 40 kDa in size were purified from adult and larval...
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Performance Evaluation of Affinity Column Mediated Immunometric Assay for Tacrolimus

Chung JW, An D, Song J, Chung HJ, Park HI, Lee W, Chun S, Min WK

BACKGROUND: Therapeutic drug monitoring (TDM) of tacrolimus is essential because of narrow therapeutic range and poor correlation of dose to blood concentration. Affinity Column Mediated Immunometric Assay (ACMIA) does not...
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Purification and biological activity of recombinant human bone morphogenetic protein-2 produced by E. coli expression system

Choi KH, Moon K, Kim SH, Yun JH, Jang KL, Cho KS

PURPOSE: Bone morphogenetic protein-2(BMP-2) has been shown to possess significant osteoinducitve potential. There have been attempts to overcome a limitation of mass production, and economical efficiency of BMP. The aim...
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Childbearing women of twenty and under are at greater risk than those of twenty-five and over for compromised folate status

Kim HA, Choi JH, Lim HS

  • KMID: 2313323
  • Nutr Res Pract.
  • 2007 Dec;1(4):254-259.
This study assessed folate intakes, folate concentrations in plasma and erythrocytes, plasma total homocysteine (tHcy) concentration, and urinary excretion of folate metabolites in Korean women with childbearing potential. A total...
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Purification and characterization of two larval glycoproteins from the cattle tick, Boophilus annulatus

El Hakim A, Shahein YE, Abouelella AM, Selim ME

The present study was conducted to identify new target immunogenic molecules from the larval stage of the cattle tick, Boophilus annulatus (Acari: Ixodidae). Two specific larval glycoproteins (GLPs) were isolated...
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Production of monoclonal antibodies against serum immunoglobulins of black rockfish (Sebastes schlegeli Higendorf)

Shin G, Lee H, Palaksha K, Kim Y, Lee E, Shin Y, Lee E, Park K, Jung T

The present study was undertaken to produce monoclonal antibodies (MAbs) against immunoglobulin (Ig) purified from black rockfish (Sebastes schlegeli Higendorf) serum using protein A, mannan binding protein, and goat IgG...
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Production of Antibody against Helicobacter pylori HP0231

Lee J, Lee SY, Lee JH

BACKGROUND: Stool antigen detection kits for diagnosis of infection of Helicobacter pylori have been widely used for their convenience, but are mostly imported. Since Helicobacter pylori strains show a distinctive...
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Application of Hemin-Agarose Affinity Chromatography to Enrich Proteome Components of Helicobacter pylori Strain 26695

Kang HL, Baik SC

  • KMID: 1574111
  • J Bacteriol Virol.
  • 2005 Jun;35(2):77-85.
The whole cell extract of Helicobacter pylori strain 26695 was treated with the hemin-agarose resin and the bound fraction was analyzed by 2-Dimensional electrophoresis. The 2-D-PAGE-displayed spots were eluted and...
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Expression of Toll-like Receptors on the Macrophages Activated by Bacterial Superantigens

Kim HY, Cho HC, Kim SK, Shin KC

  • KMID: 2285109
  • Infect Chemother.
  • 2004 Oct;36(5):286-293.
BACKGROUND: Staphylococcal enterotoxin B (SEB) as a prototype superantigen is known to play a pivotal role in toxic shock syndrome and severe sepsis. However, the precise mechanism initiating the activation...
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