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Interaction between Brucella melitensis 16M and small ubiquitin-related modifier 1 and E2 conjugating enzyme 9 in mouse RAW264.7 macrophages

Yi J, Wang Y, Li Q, Zhang H, Shao Z, Deng X, He J, Xiao C, Wang Z, Wang Y, Chen C

Brucella is an intracellular pathogen that invades a host and settles in its immune cells; however, the mechanism of its intracellular survival is unclear. Modification of small ubiquitin-related modifier (SUMO)...
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A Case of Catabacter hongkongensis and Alistipes indistinctus Isolated from Blood Cultures of a Patient with Acute Appendicitis

Ryu J, Kim Y, Lee J, Cho SY, Park TS, Lee HJ

Catabacter hongkongensis is an anaerobic gram-positive coccobacillus that was first isolated in Hong Kong. It is infectious and causes high mortality in patients with rare but underlying diseases. Alistipes indistinctus...
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Membranoproliferative glomerulonephritis in a patient with chronic brucellosis

Provatopoulou S, Papasotiriou M, Papachristou E, Gakiopoulou H, Marangos M, Goumenos D

Brucellosis is the most common zoonotic disease in Greece, with an endemic distribution and can affect any organ. Infiltration of the renal parenchyma causes acute and chronic interstitial nephritis with...
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Prevalence state of canine brucellosis in South Korea during 2015 and 2016

Jung JY, Yoon SS, Lee SH, Park JW, Lee JJ, Her M, So B, Kim JH

We investigated the prevalence of canine brucellosis in companion and stray dogs between March 2015 and December 2016 and determined the disease characteristics based on the geographic distribution, sex and...
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Vaccination of goats with a combination Salmonella vector expressing four Brucella antigens (BLS, PrpA, Omp19, and SOD) confers protection against Brucella abortus infection

Leya M, Kim WK, Cho JS, Yu EC, Kim YJ, Yeo Y, Lyoo KS, Yang MS, Han SS, Lee JH, Tark D, Hur J, Kim B

Salmonella is an intracellular pathogen with a cellular infection mechanism similar to that of Brucella, making it a suitable choice for use in an anti-Brucella immune boost system. This study...
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An Imported Case of Brucella melitensis Infection in South Korea

Lee JY, Jeon Y, Ahn MY, Ann HW, Jung IY, Jung W, Kim MH, Ahn JY, Song JE, Kim YC, Oh DH, Kim EJ, Jeong SJ, Ku NS, Kim H, Lee K, Kim JM, Choi JY

Brucellosis is a zoonotic infection that is usually transmitted from cattle to humans through ingestion of animal milk, direct contact with animal parts, or inhalation of aerosolized particles. In Korea,...
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Different invasion efficiencies of Brucella abortus wild-type and mutantsin RAW 264.7 and THP-1 phagocytic cells and HeLa non-phagocytic cells

Shim S, Im YB, Jung M, Park WB, Yoo HS

Brucellosis is one of the common zoonoses caused by Brucella abortus (B. abortus). However, little has been reported on factors affecting invasion of B. abortus into host cells. To investigate...
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Effect of immunization routes and protective efficacy of Brucella antigens delivered via Salmonella vector vaccine

Lalsiamthara J, Won G, Lee JH

An anti-Brucella vaccine candidate comprised of purified Brucella lipopolysaccharide (LPS) and a cocktail of four Salmonella Typhimurium (ST)-Brucella vectors was reported previously. Each vector constitutively expressed highly conserved Brucella antigens...
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Analysis of protein expression in Brucella abortus mutants with different growth rates by two-dimensional gel electrophoresis and LC-MS/MS peptide analysis

Park WB, Im YB, Shim S, Yoo HS

Brucella abortus is a bacterium that causes brucellosis and is the causative agent of worldwide zoonoses. Pathogenesis of the B. abortus infection is complicated, and several researchers have attempted to...
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Tannic acid-mediated immune activation attenuates Brucella abortus infection in mice

Reyes AW, Hop HT, Arayan LT, Huy TX, Min W, Lee HJ, Chang HH, Kim S

Brucellosis is an emerging infectious disease affecting humans and animals. In this study, we investigated the in vitro and in vivo effects of tannic acid (TA) against Brucella abortus infection....
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Development and trial of vaccines against Brucella

Lalsiamthara J, Lee JH

The search for ideal brucellosis vaccines remains active today. Currently, no licensed human or canine anti-brucellosis vaccines are available. In bovines, the most successful vaccine (S19) is only used in...
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Seroprevalence of Brucellosis in Human Immunodeficiency Virus Infected Patients in Hamadan, Iran

Keramat F, Majzobi MM, Poorolajal J, Ghane ZZ, Adabi M

OBJECTIVES: Brucellosis is a systemic disease with a wide spectrum of clinical manifestations. This study aimed to determine the seroprevalence of brucellosis in human immunodeficiency virus (HIV) infected patients in...
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A Novel PCR Assay for Detecting Brucella abortus and Brucella melitensis

Alamian S, Esmaelizad M, Zahraei T, Etemadi A, Mohammadi , Afshar D, Ghaderi S

OBJECTIVES: Brucellosis is a major zoonotic disease that poses a significant public health threat worldwide. The classical bacteriological detection process used to identify Brucella spp. is difficult and time-consuming. This...
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A Comparative Study of Helicobacter pylori Growth on Different Agar-based Media

Lee JH, Park J, Park MR, Na YH, Cho SJ

BACKGROUND/AIMS: Optimal culture conditions for Helicobacter pylori have not been established. We compared the effectiveness of four different agar-based media for the growth of H. pylori. MATERIALS AND METHODS: G27, ATCC...
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Diagnostic role of mean platelet volume and neutrophil to lymphocyte ratio in childhood brucellosis

Bozdemir S, Altıntop , Uytun S, Aslaner H, Torun YA

BACKGROUND/AIMS: Brucellosis patients present various non-specific clinical symptoms, such as fever, fatigue, sweating, joint pain, arthritis, myalgia, and headache. Based on the nonspecifity of its clinical signs and symtoms, we...
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Inhibitory effect of red ginseng acidic polysaccharide from Korean red ginseng on phagocytic activity and intracellular replication of Brucella abortus in RAW 264.7 cells

Bernardo Reyes AW, Simborio H, Hop HT, Arayan LT, Min WG, Lee HJ, Rhee MH, Chang HH, Kim S

Korean red ginseng (KRG) has long been used in traditional Korean and Oriental medicine. However, the anti-bacterial mechanism and therapeutic efficiency of KGR for intracellular Brucella infection are still unclear....
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Isolation and characterization of Brucella abortus isolates from wildlife species in South Korea

Truong QL, Kim K, Kim JT, Her M, Jung SC, Hahn TW

A total of 782 blood and 465 tissue samples from 1,039 wild animals and 127 dairy goats were collected from January 2011 to December 2013 in 10 provinces of South...
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First Case of Human Brucellosis Caused by Brucella melitensis in Korea

Kim HN, Hur M, Moon HW, Shim HS, Kim H, Ji M, Yun YM, Kim SY, Um J, Lee YS, Hwang SD

No abstract available.
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Molecular cloning, purification and immunogenicity of recombinant Brucella abortus 544 malate dehydrogenase protein

Reyes AW, Simborio H, Hop HT, Arayan LT, Kim S

The Brucella mdh gene was successfully cloned and expressed in E. coli. The purified recombinant malate dehydrogenase protein (rMDH) was reactive to Brucella-positive bovine serum in the early stage, but...
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In silico analysis of Brucella abortus Omp2b and in vitro expression of SOmp2b

Golshani M, Vaeznia N, Sahmani M, Bouzari S

PURPOSE: At present, there is no vaccine available for the prevention of human brucellosis. Brucella outer membrane protein 2b (Omp2b) is a 36 kD porin existed in common Brucella pathogens...
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