Korean J Transplant.  2021 Oct;35(Supple 1):S163. 10.4285/ATW2021.PO-1123.

The unique changes of lung microbiome in chronic lung allograft dysfunction

• Yeo HJ
• Cho WH
• Kim D
• Kim YH
• Yu Y

• KMID: 2521312
• DOI: http://doi.org/10.4285/ATW2021.PO-1123

Abstract

Background
Both unique bacterial populations and longitudinal changes in the lung microbiome may play a critical role in the development of chronic lung allograft dysfunction (CLAD).
Methods
We evaluated the difference in lung microbiome associated with CLAD through the lung microbiome analysis of the donor ̕ s lung at the time of transplantation and after CLAD occurrence.
Results
A significant change of bacterial diversity was observed in the lung microbiomes of CLAD compared to at the time of transplantation. Depending on the severity of CLAD, the bacterial diversity tended to decrease. In all lung microbiomes, Actinobaceria, Fimicutes, and Proteobacteria were highly abundant and account for approximately 75% of the total bacterial community. Pseudomonas, Xanthomonas, Bacillus, Pasteurella, and Rhodococcus occupied a large proportion. Compared to the time of transplantation, more Klebsiella was observed in lung microbiomes of CLAD. Genera such as Aerococcus, Caldiericum, Croceibacter, Leptolyngbya, and Pulveribacter were uniquely identified in CLAD, whereas there were no taxa identified at time of transplantation. In particular, seven taxa including Croceibacter atlanticus, Caldisericum exile, Dolichospermum compactum, Stappia sp. ES.058, kinetoplastibacterium sorsogonicusi, Pulveribacter suum were identified uniquely in CLAD.
Conclusions
Compared to the point of transplantation, the change of bacterial diversity in the lung microbiomes of CLAD was observed, and the seven taxa were uniquely identified in CLAD.