Ann Clin Microbiol.  2016 Sep;19(3):59-64. 10.5145/ACM.2016.19.3.59.

Evaluation of Diagnostic Performance of RAPIDEC CARBA NP Test for Carbapenemase-Producing Enterobacteriaceae

• Song W
• Yoo G
• Hwang GY
• Uh Y

• KMID: 2353041
• DOI: http://doi.org/10.5145/ACM.2016.19.3.59

Abstract

BACKGROUND
Extended-spectrum β-lactamase (ESBL)-producing Enterobacteriaceae are resistant to most β-lactam antibiotics except carbapenems. In recent years, infrequently isolated Enterobacteriaceae that produce carbapenemase pose a serious threat in the selection of appropriate therapeutic antibiotics. The rapid detection method of carbapenemase-producing Enterobacteriaceae (CPE) is necessary to prevent the spread of CPE into healthcare facilities.
METHODS
One hundred clinical Enterobacteriaceae isolates (Klebsiella pneumoniae 40, Escherichia coli 40, others 20) showing susceptibility to carbapenems and positivity in the CLSI ESBL phenotypic test from November 2015 to March 2016 and 59 stocked Enterobacteriaceae isolates harboring resistance genes producing carbapenemase (K. pneumoniae 56, Enterobacter cloacae 2, E. coli 1; types of CPE: KPC 36, GES 12, NDM 6, VIM 2, OXA 2, IMP 1) were subjected to the RAPIDEC CARBA NP test (bioMérieux, France) and CPE phenotypic test using the modified Hodge test (MHT) and carbapenemase inhibition test.
RESULTS
All of the 100 Enterobacteriaceae isolates with carbapenem susceptibility and ESBL positivity were negative on the RAPIDEC CARBA NP test and CPE phenotypic test. Of 59 stock CPE isolates, 53 and 42 showed positive results to the RAPIDEC CARBA NP test and MHT, respectively. The sensitivity and specificity of the RAPIDEC CARBA NP test for detecting CPE were 89.8% and 100%, respectively.
CONCLUSION
The RAPIDEC CARBA NP test is simple and produces a result within 3 hr. In conclusion, the test is a useful screen for detecting CPE because it shows high sensitivity and specificity for CPE detection.

Keyword

Carbapenem; Carbapenemase; Enterobacteriaceae; Modified Hodge test

MeSH Terms

Anti-Bacterial Agents
Carbapenems
Delivery of Health Care
Enterobacter cloacae
Enterobacteriaceae*
Escherichia coli
Methods
Pneumonia
Sensitivity and Specificity
Anti-Bacterial Agents
Carbapenems

Figure

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  Fig. 1. Phenotype results of KPC-producing K. pneumoniae. (A) Positive finding in RAPIDEC CARBA NP,(B) Positive finding in MHT and CIT. Abbreviations: P, phenylboronic acid disk; M, meropenem disk; E, EDTA disk.

Reference

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