Ewha Med J.  2013 Mar;36(1):35-42. 10.12771/emj.2013.36.1.35.

Microarray Analysis after Intravenous Immunoglobulin Treatment in Patients with Kawasaki Disease

• Lee HY
• Kwon JH
• Kim HS
• Sohn S
• Hong YM

• KMID: 2284013
• DOI: http://doi.org/10.12771/emj.2013.36.1.35

Abstract

OBJECTIVES
The etiology for Kawasaki disease (KD) remains unknown, but several studies have suggested the involvement of immune dysregulation and genetic factors. The purpose of this study is to compare gene expressions before and after an infusion of intravenous immunoglobulin (IVIG) in KD patients.
METHODS
Blood was obtained from both acute and sub-acute phases of 4 patients with KD and febrile control children. Blood was collected in PAXgene blood RNA tubes and RNA was extracted using a PAXgene blood RNA isolation kit. Labeled RNAs were analyzed using Roche NimbleGen human whole genome 12-plex array.
RESULTS
KD patients prior to IVIG injection showed more than a two-fold increase in the expression of 88 genes and more than a two-fold decrease in the expression of 98 genes compared to the control group. They also showed more than two-fold increase in the expression of 226 genes and more than a two-fold decrease in 117 genes in KD patients after IVIG treatment compared to the patients before IVIG injection. Through microarray evaluation, the expressions of genes involved in proliferation, translation, inflammatory response, immune response, cell adhesion, cell migration, cell differentiation, apoptosis, cell growth, transport, cell cycle, transcription, signal transduction and metastasis were observed.
CONCLUSION
Changes in gene expressions in pediatric patients with KD before and after IVIG were observed via microarray evaluation.

Keyword

Gene expression; Intravenous immunoglobulin; Kawasaki disease; Microarray analysis

MeSH Terms

Apoptosis
Cell Adhesion
Cell Cycle
Cell Differentiation
Cell Movement
Child
Gene Expression
Genome
Humans
Immunoglobulins
Immunoglobulins, Intravenous
Microarray Analysis
Mucocutaneous Lymph Node Syndrome
Neoplasm Metastasis
RNA
Signal Transduction
Immunoglobulins
Immunoglobulins, Intravenous
RNA

Figure

• 

  Fig. 1 Gene heat map in KD patients Pre- and Post-IVIG treatment. The color red indicates over expression while green indicates down expression. KD, Kawasaki disease; IVIG, intravenous immunoglobulin.

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