Korean J Pediatr Gastroenterol Nutr.  2011 Jun;14(2):148-154. 10.5223/kjpgn.2011.14.2.148.

Distribution of Disease Severity and Group A Rotavirus Genotypes (VP7 & VP4) in Children with Acute Rotavirus Gastroenteritis

• Oh HJ
• Kang HS
• Kang KS
• Kim YW
• Hong JY
• Shin KS
• Lee JS
• Lee SH
• Lee KH
• Cho M
• Suh DI

• KMID: 2276535
• DOI: http://doi.org/10.5223/kjpgn.2011.14.2.148

Abstract

PURPOSE
We aimed to study the distribution of rotavirus genotypes (VP7 and VP4) and disease severity of rotavirus gastroenteritis prevalent in our community.
METHODS
Stool samples were collected from 156 children who were hospitalized with rotavirus gastroenteritis from December 2007 to June 2008. The disease severity of all patients was scored using the Vesikari scale. After extraction of ds-RNA of the rotavirus, cDNA synthesis using reverse transcription and polymerase chain reaction (RT-PCR) and multiplex PCR was performed. Following this, the final identification of genotypes was performed.
RESULTS
Of the 156 samples, VP7(G) and VP4(P) genotypes were identified in 147 (94.2%) and 140 (89.7%) samples, respectively. G1 (116 of 147 samples; 78.9%) and P[8] (137 of 140 samples; 97.9%) were the most prevalent, respectively. Of the 138 samples identified of combination types of VP7 and VP4, G1P[8] (111 samples; 80.4%) was the most prevalent. Other combination types varied with very low distribution rates. 9.4% of genotypes were not included in the new vaccines. The disease severity score was 11.8+/-3.3 (mean+/-2SD). The distribution of disease severity was mild or moderate in 37.8% and severe in 62.2% of patients.
CONCLUSION
The most prevalent genotype combination of rotavirus was G1P[8] and genotypes not included in the vaccines represented 9.4% in our community. Disease severity distribution of hospitalized children with rotavirus gastroenteritis was higher in the severe than in the mild and moderate categories.

Keyword

Rotavirus; Genotype; Severity

MeSH Terms

Child
Child, Hospitalized
DNA, Complementary
Gastroenteritis
Genotype
Humans
Multiplex Polymerase Chain Reaction
Polymerase Chain Reaction
Reverse Transcription
Rotavirus
Vaccines
DNA, Complementary
Vaccines

Figure

• 

  Fig. 1 Distribution of VP7 genotypes of Rotavirus isolated from 147 patients. G1 was the most common, identified in 116 patients (78.9%).

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  Fig. 2 Distribution of VP4 genotypes of Rotavirus isolated from 140 patients. P[8] was the most common, identified in 137 patients (97.9%).

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