Korean J Nephrol.  1997 Mar;16(1):101-105.

Comparison of Flow Cytometry Crossmatch with Conventional Lymphocytotoxic Crossmatch in Living Donor Renal Transplantation

• Shin YS
• Kim YO
• Yang CW
• Jin DC
• Kim SY
• Choi EJ
• Chang YS
• Pahk YJ
• Bang BK

Abstract

This study was performed to compare the sensitivity of flow cytometry crossmatch(FCXM) with conventional lymphocytotoxic crossmatch(CXM), and its clinical impact. Total 174 pair of sera from potential living renal transplant donors and recipients were tested from Jan. 5, 1995 to April 3, 1996 with conventional CXM and FCXM at Kangnam St. Mary's hospital. Of 174 potential living donor/ recipient pairs, two (1.1%) had positive warm T (TW)/CXM, 3(1.7%) positive warm B(BW)/CXM, and 5(2.9%) positive warm TandB/CXM. Of 164 potential CXM-negative living donor/recipient pairs evaluated with FCXM, ten (6%) had positive T/ FCXM, and thirty one (19%) positive B/FCXM. Of 45 living donor renal transplantation with negative TW/CXM, one (2.2%) had a positive BW/CXM, 11 (24.4%) positive B/FCXM, and 2 (4.4%) positive T/ FCXM. They had been followed up over 3 months. Acute rejection episodes were observed in 8 patients. Of these patients, two had positive B/FCXM and one had a positive T/FCXM. In conclusion, FCXM is more sensitive than conventional CXM. Further follow-up study is necessary to know whether this higher sensitivity leads to fewer graft rejection.

Keyword

Flow cytometry crossmatch; Lymphocytotoxic crossmatch; Living-related renal transplantaton; Acute rejection

MeSH Terms

Flow Cytometry*
Follow-Up Studies
Graft Rejection
Humans
Kidney Transplantation*
Living Donors*
Tissue Donors