Clin Exp Otorhinolaryngol.  2016 Mar;9(1):14-20. 10.21053/ceo.2016.9.1.14.

The Effect of Dexpanthenol on Ototoxicity Induced by Cisplatin

• Toplu Y
• Sapmaz E
• Parlakpinar H
• Kelles M
• Kalcioglu
• Tanbek K
• Kizilay A

• KMID: 2166276
• DOI: http://doi.org/10.21053/ceo.2016.9.1.14

Abstract

OBJECTIVES
This study was aimed to investigate the protective effects of dexpanthenol (Dxp) on against cisplatin-induced ototoxicity.
METHODS
To examine this effect, distortion product otoacoustic emissions (DPOAEs) measurements and serum levels of oxidative and antioxidant status (including malondialdehyde, superoxide dismutase, catalase, glutathione, glutathione peroxidase, total oxidant status, total antioxidant status, and oxidative stress index) were evaluated. Thirty-two adult female Wistar albino rats were randomly divided into 4 equal groups; control (K), cisplatin (C), cisplatin plus Dxp (CD), and Dxp (D). In all groups DPOAEs measurements, between 996 and 10,078 Hz as DPOAEs and input/output functions, were performed on days 0, 1th, 5th, and 12th. Prior to death, the last DPOAEs measurements and blood samples were taken.
RESULTS
In the C group, statistically significant differences were detected at all frequencies between 0 and 5 days and 0 and 12 days measurements (P<0.05). Serum level of oxidant and antioxidant status were detected statistically significantly changed in this group versus K group (P<0.05). Contrary to the C group, in the CD group hearing ability was seen largely preserved at many frequencies and serum levels of all biochemical parameters were shifted toward normal values, similar to the K group. No significant differences were detected in the either D or K group's measurements.
CONCLUSION
According to these results, Dxp may prevent cisplatin-induced ototoxicity.

Keyword

Cisplatin; Ototoxicity; Dexpanthenol; Otoacoustic Emissions; Biomarkers

MeSH Terms

Adult
Animals
Biomarkers
Catalase
Cisplatin*
Female
Glutathione
Glutathione Peroxidase
Hearing
Humans
Malondialdehyde
Oxidative Stress
Rats
Reference Values
Superoxide Dismutase
Catalase
Cisplatin
Glutathione
Glutathione Peroxidase
Malondialdehyde
Superoxide Dismutase

Figure

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  Fig. 1. Distortion product otoacoustic emission (DPOAE) amplitudes obtained from on day zero. C, cisplatin; D, dexpanthenol; CD, dexpanthenol plus cisplatin; SPL, sound pressure level.

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  Fig. 2. Distortion product otoacoustic emission (DPOAE) amplitudes obtained on day 1. C, cisplatin; D, dexpanthenol; CD, dexpanthenol plus cisplatin; SPL, sound pressure level.

• 

  Fig. 3. Distortion product otoacoustic emission (DPOAE) amplitudes obtained on day 5. C, cisplatin; D, dexpanthenol; CD, dexpanthenol plus cisplatin; SPL, sound pressure level.

• 

  Fig. 4. Distortion product otoacoustic emission (DPOAE) amplitudes obtained on day 12. C, cisplatin; D, dexpanthenol; CD, dexpanthenol plus cisplatin; SPL, sound pressure level.

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  Fig. 5. Variations in amplitudes of distortion product otoacoustic emission (DPOAE) for different time points in cisplatin (C) group (C0, baseline [day 0]; C1, 1st day: C5, 5th day; C12, 12th day). SPL, sound pressure level.

• 

  Fig. 6. Variations in amplitudes of distortion product otoacoustic emission (DPOAE) for different time points in cisplatin plus dexpanthenol (CD) used group (CD0, baseline [day 0]; CD1, 1st day; CD5, 5th day; CD12, 12th day). SPL, sound pressure level.

• 

  Fig. 7. Variations in amplitudes of distortion product otoacoustic emission (DPOAE) for different time points in dexpanthenol (D) group (D0, baseline [day 0]; D1, 1st day; D5; 5th day; D12, 12th day). SPL, sound pressure level.

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