Increased Serum 8-hydroxy-Guanine Levels in Diabetic Patients

Moon, Byung Sool; Lee, Yun Yong; Shin, Chan Soo; Park, Kyong Soo; Kim, Seong Yeon; Lee, Hong Kyu; Park, Su Jin; Chung, Myung Hee

Abstract

BACKGROUND: Production of reactive oxygen species(ROS) increased in diabetic patients and oxidative damage may contribute to the development of diabetic complications. 8-OH-deoxyGuanosine (oh8dG) and 8-OH-Guanine(ohSGua) are known as excellent markers of the oxidative darnage to DNA. This study was performed to investigate whether serum 8-OH-guanine increased in diabetic patients and whether the glycemic control(HbAlc) is related to the levels of serum 8-OH-guanine. METHOD: In this study, 28 patients with diabetes mellitus was studied, We also included 27 nondiabetic healthy controls whose age, sex, and BMI were matehed to the diabetic patients. Serum 8-OH-Guanine was assayed by high performance liquid chromatography after antibody-based purification with monoclonal antibodies to S-OH-Guanine.
RESULTS
The levels of serum 8-OH-Guanine was significantly higher in diabetic patients than in normal controls(4.02+/-3.77 pmol/mL vs. 0.89+/-0.63 pmol/ mL, p<0.01). Serum 8-OH-Guanine concentration was not related to age, HbAlc, duration of diabetes, creatinine clearance, total cholesterol, triglyceride, and HDL-cholesterol. Conclusions: We found a significant increase in serum 8-OH-guanine levels from diabetic patients compared with their respective controls. These results suggest that diabetic patients have significantly increased oxidatively damaged DNA. The factors regulating the oxidative damage to DNA should be further investigated.