J Korean Ophthalmol Soc.  1997 Jan;38(1):1-6.

Inhibition of Corneal Angiogenesis by Antibody to Integrin b3 Subunit

Affiliations
  • 1Depratment of Ophthalmology, Catholic University Medicine College, Seoul, Korea.
  • 2Depratment of Ophthalmology, University of California, San Francisco, CA, USA.

Abstract

Vascular endothelial cell expression of avb3, and integrin receptor that binds von Willebrand factor, vitronectin, and fibrinogen, increases in response to angiogenic stimuli such as basic fibroblast growth factor(bFGF) and during capillary proliferation in vivo. We investigated the importance of avb3 function during bFGF-induced corneal angiogenesis by examining the effects of 9D491, a monoclnal natibody against b3 that blocks avb3-mediated cell adhesion to vitronectin and fibrinogen in vitro. A hydrogel disk containing 500ng of bFGF was implanted into the superior corneal stroma of each of twelve New Zealand white rabbit eyes. Each eye also received a second hydrogel disk placed adjacent of the firtt, randomized to contain either 3ug of 9D491 mAb(n=6) or 6E10, an irrelevant antibody of the same isotype, (n=6). Both disks were positioned 1.2mm from the superior limbus. Eyes were examined daily under a streomicroscope by two masked observers and counted an angiogenesis score based on number and length of new blood vessels. On days 5 through 7 post-implantation, angiogenesis scores were significantly lower in eyes treated with avb3 mAb(averaged score=3.3) as compared to eyes treated with 6E10(averaged score=8.0)(p<0.002, Wilcoxon rank sum test). In a rabbit corneal pocket model of angiogenesis, neutralizing monoclonal antibody to b3 inhibits corneal angiogenesis induced by bFGF. Substances that target the integrin b3 subunit may have therapeutic potential in disorders characterized by ocular neovascularization.

Keyword

Angiogenesis; Corneal pocket assay; Integrin b3

MeSH Terms

Blood Vessels
Capillaries
Cell Adhesion
Corneal Neovascularization*
Corneal Stroma
Endothelial Cells
Fibrinogen
Fibroblasts
Hydrogel
Masks
New Zealand
Vitronectin
von Willebrand Factor
Fibrinogen
Hydrogel
Vitronectin
von Willebrand Factor
Full Text Links
  • JKOS
Actions
Cited
CITED
export Copy
Close
Share
  • Twitter
  • Facebook
Similar articles
Copyright © 2024 by Korean Association of Medical Journal Editors. All rights reserved.     E-mail: koreamed@kamje.or.kr