Korean J Obstet Gynecol.  2011 Apr;54(4):184-191. 10.5468/KJOG.2011.54.4.184.

The prognostic significance of p16, Ki-67, p63, and CK17 expression determined by immunohistochemical staining in cervical intraepithelial neoplasia 1

• Kim SM
• Lee JU
• Lee DW
• Kim MJ
• Lee HN

• KMID: 2013233
• DOI: http://doi.org/10.5468/KJOG.2011.54.4.184

Abstract

OBJECTIVE
To evaluate the prognostic significance of p16, Ki-67, p63, and cytokeratin (CK) 17 expression determined by immunohistochemical staining in cervical intraepithelial neoplasia (CIN) 1.
METHODS
Biopsy tissue samples from 33 patients diagnosed with CIN 1 were stained immunohistochemically for p16, Ki-67, p63, and CK17. The staining results were correlated with the clinical course of the disease.
RESULTS
Seventeen of 18 (94.4%) p16-negative patients experienced regression, and only 1 patient (5.6%) developed persistent disease. Fifteen of the 16 (93.8%) Ki-67-negative patients experienced regression, and 1 patient (6.3%) developed persistent disease. Negative p16 and Ki-67 expression correlated significantly with disease regression (P=0.004 and P=0.017, respectively). Fourteen of 15 (93.3%) patients negative for both p16 and Ki-67 experienced regression, and 1 patient negative for both p16 and Ki-67 (6.7%) developed persistent disease. The expression levels of p63 and CK17 were not significantly associated with disease regression or persistence (P=0.149 and P=0.642, respectively). Ten of the 13 (76.9%) p16-positive patients had a high-risk HPV infection. High-risk HPV infection was significantly associated with p16 expression (P=0.049).
CONCLUSION
CIN 1 with p16- or Ki-67-negative immunohistochemical staining was associated with spontaneous disease regression. The p63 and CK17 expression patterns were not related to the behavior of CIN 1.

Keyword

Cervical intraepithelial neoplasia 1; p16; Ki-67; p63; Cytokeratin 17

MeSH Terms

Biopsy
Cervical Intraepithelial Neoplasia
Humans
Keratin-17
Keratins
Keratin-17
Keratins

Figure

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  Fig. 1. (A) H&E, ×100, (B) H&E, ×200. The expression of p16 (C, D), Ki-67 (E, F), and p63 (G, H) in the lower one third of the cervical epithelium (×100, ×200). Note the loss of the CK17 expression in the lower one third of the cervical epithelium (I, J) ×100, ×200. (C-J) immunohistochemical stain.

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  Fig. 2. The association of positive P16, Ki-67, p63, and CK17 immunohistochemical expressions with high risk HPV infection. aPearson's chi-square test, bFisher's exact test.

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