Korean J Urol.  2006 Apr;47(4):347-352. 10.4111/kju.2006.47.4.347.

The Clinical Significance of the Expression of Matrix Metalloproteinase-2 and Matrix Metalloproteinase-9 in Renal Cell Carcinoma

Affiliations
  • 1Departments of Urology, Yeungnam University College of Medicine, Daegu, Korea.
  • 2Departments of Biochemistry and Molecular Biology, Yeungnam University College of Medicine, Daegu, Korea.

Abstract

PURPOSE
Matrix metalloproteinases (MMPs) are endogenous peptidases that are capable of degrading various components of the basement membranes. To evaluate the clinical significance of the expressions of MMPs in renal cell carcinomas (RCCs), the MMPs' expression in RCCs and non- neoplastic kidney tissues was examined to evaluate the clinical significance of the expressions of MMPs in renal cell carcinomas (RCCs).
MATERIALS AND METHODS
Twenty-two patients with RCCs (the RCC group), and eleven patients with non-neoplastic kidneys (the control group), were enrolled in this study between November 2002 and November 2003. The MMP-2 and MMP-9 activities were estimated using gelatin zymography, and they were quantified using a laser densitometer. The results were compared with the clinicopathological characteristics.
RESULTS
The expression of MMP-9 was significantly elevated in the RCC group compared with the control group (p<0.01). There was no difference in MMP-2 activity between the RCC group and the control group (p>0.05). The levels of MMP-9 expression in the RCC patients with a large tumor (>4cm) or vascular invasion were significantly higher than that in the patients without these clinical manifestations (p<0.01). There were also significant differences in the expression of MMP-9 among the T stages (p<0.01).
CONCLUSIONS
The present study shows a close relationship between the expression of MMP-9 and the tumor size and tumor stage in RCC. MMP-9 may be used as a prognostic marker and for the development of a novel treatment modality for RCC.

Keyword

Matrix metalloproteinases; Renal cell carcinoma

MeSH Terms

Basement Membrane
Carcinoma, Renal Cell*
Gelatin
Humans
Kidney
Matrix Metalloproteinase 2*
Matrix Metalloproteinase 9*
Matrix Metalloproteinases
Peptide Hydrolases
Gelatin
Matrix Metalloproteinase 2
Matrix Metalloproteinase 9
Matrix Metalloproteinases
Peptide Hydrolases

Figure

  • Fig. 1 Expression of MMP-2 and MMP-9 in the patients with RCC and the control group. The expression of MMP-9 is significantly elevated in the RCC group compared with the control group (p<0.01). There is no difference in MMP-2 activity between the RCC group and the control group (p>0.05). *: p<0.01 compared with the control values; Mann-Whitney test. Au: arbitrary units, MMP: matrix metalloproteinase, RCC: renal cell carcinoma.

  • Fig. 2 Gelatin zymography for MMP-2 and MMP-9. The protein isolated from the homogenate are analyzed for the MMP-9 and MMP-2 expression by gelain zymography. The standard shows bright bands of MMP-9 (active) and MMP-2 (pro-and active). Normal kidney tissues (N1 and N2) show faint reaction bands for pro-MMP-9. Tumor samples of RCC (T1-T5) show apparent gelatinolytic activity of pro-MMP-9. Notice a strong expression of pro-MMP-9 in the T stage III with renal vein thrombosis (T1 and T4). Standard: conditioned media from the HT1080 culture, MMP: matrix metalloproteinase, RCC: renal cell carcinoma.


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