Korean J Pediatr Gastroenterol Nutr.
2001 Apr;4(1):28-33.
A Possible Relation of the Helicobacter pylori pfr Gene to Iron Deficiency Anemia?
- Affiliations
-
- 1Department of Pediatrics, Inha University Hospital, Inchon, Korea. yhchoe@inha.ac.kr
- 2Department of Pathology, Inha University Hospital, Inchon, Korea.
Abstract
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PURPOSE: H. pylori infection is thought to contribute to iron-deficiency anemia, especially during
puberty. The ferritin protein Pfr of H. pylori is homologous to eukaryotic and prokaryotic ferritins.
The purpose of this study was to analyze the H. pylori pfr status in gastric biopsy specimens
according to clinical data, including antral gastritis with or without iron-deficiency anemia.
METHODS
A total of 26 H. pylori-positive patients aged from ten to 18 years were categorized
into subgroups based on the presence or absence of iron-deficiency anemia. All of them had antral
gastritis. Sixteen patients were proved to have iron-deficiency anemia by hematological study, two
of which had a duodenal ulcer. The other ten patients showed normal hematological findings. DNA
isolation was performed from each of the gastric biopsy specimens. PCR amplification of the pfr
gene coding was done using two sets of primers. The pfr region, 501 bp, was generated by linking
the sequences of the two PCR products. The nucleotide and protein sequences were compared
between the pfr regions from Korean H. pylori strains and the NCTC 11638, 26695, and J99 strain,
which were obtained from the Genbank. Sequence comparisons were also performed for the pfr
regions between the iron-deficiency anemia (+) and (-) groups.
RESULTS
Analysis of the complete coding region of pfr gene revealed three sites of mutation. The
Ser39Ala mutation was found in 100% (26/26), Gly111Asn in 26.9% (7/26), and Gly82Ser in 11.5%
(3/26). There were no significant differences in the mutations of the pfr regions between the iron
deficiency anemia (+) and (-) groups.
CONCLUSION
The mutation in the pfr gene did not relate with the clinical phenotype, iron deficiency
anemia. Further studies are needed on the aspects of host side or other complex factors to elucidate
anemia. Further studies are needed on the aspects of host side or other complex factors to elucidate
the mechanisms by which the H. pylori infection might lead to iron deficiency anemia.