Infect Chemother.  2009 Jun;41(3):181-184. 10.3947/ic.2009.41.3.181.

Rapid Detection of Extended Spectrum beta-Lactamase (ESBL) for Enterobacteriaceae by use of a Multiplex PCR-based Method

• Kim J
• Jeon S
• Rhie H
• Lee B
• Park M
• Lee H
• Lee J
• Kim S

• KMID: 1782321
• DOI: http://doi.org/10.3947/ic.2009.41.3.181

Abstract

A multiplex PCR method has been developed to classify extended spectrum beta-lactamase (ESBL) and plasmid-mediated AmpC beta-lactamase (PABL). This method consists of the use of two four-multiplex PCRs for the detection of TEM, OXA, SHV, CTX-M, CMY, and DHA type beta-lactamases. We have compared findings from the use of conventional detection methods with that of this newly developed typing method. In testing for 73 ESBL-producing and PABL-producing isolates, 100% of the isolates were correctly identified as previously characterized types and, 44 types of beta-lactamases were additionally identified from 33 isolates. This assay not only reduces the time for classification but also increases the accuracy for detection.

Keyword

Extended Spectrum-beta Lactamase (ESBL); Rapid classification; Multiplex PCR

MeSH Terms

Bacterial Proteins
beta-Lactamases
Enterobacteriaceae
Multiplex Polymerase Chain Reaction
Oxytocin
Polymerase Chain Reaction
Bacterial Proteins
Oxytocin
beta-Lactamases

Figure

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  Figure 1 Amplification profiles of each primer set for the Set I and Set II multiplex assays are shown. Set I assay products are shown in the first five lanes with the primer pairs indicated above each lane. (Lane 6 was empty.) Set II assay products are shown in the next five lanes with the primer pairs indicated above each lane. The sizes of the DNA standards are indicated (in base pairs) to the left of the gel image. Amplicon sizes for the Set I reactions are indicated on the left side of the gel, while amplicon sizes for the Set IIreactions are indicated on the right side of the gel.

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