Korean J Pediatr.  2004 Jun;47(6):685-690.

Cardioprotective Effects of Minocycline in Rat Myocardiac Cell Culture Induced by Hypoxia

• Yang BS
• Park HJ
• Kim HT
• Lee JW
• Lee DS
• Nho US
• Chung HL
• Kim WT

Abstract

PURPOSE
Minocycline appears to reduce the mortality rate of patients with myocardial infarction. Therefore, the cardioprotective effects of minocycline through reduction of apoptosis were studied in a rat myocardiac cell culture induced by hypoxia.
METHODS
Cultured cells from the hearts of Sprague-Dawley rats were divided into two sets of groups: normoxia groups treated with 5% O2 and hypoxia groups treated with 1% O2. After several days of incubation, the control groups were not treated with minocycline, while the sample groups were treated with 1 mug/mL of minocycline. The damaged cells were observed under a microscope, while apoptosis was detected using a Tdt-mediated dUTP nick and labeling(TUNEL) assay control-stained with 4'-6'-diamidino-2-phenylindole-2HCl(DAPI).
RESULTS
In normoxia, the control group showed a statistically significant difference from the sample group treated with 1 mug/mL of minocycline. In addition, in hypoxia, there was also a statistically significant difference between the two groups. When comparing the normoxia and hypoxia groups to each other, there was a statistically significant difference between the control groups and sample groups.
CONCLUSION
Minocycline was found to be protective for heart in normoxia and in hypoxia in the model of rat myocardiac cell culture.

Keyword

Minocycline; Hypoxia; Myocardial infarction; Myocardiac cells; Cultured

MeSH Terms

Animals
Anoxia*
Apoptosis
Cell Culture Techniques*
Cells, Cultured
Heart
Humans
Minocycline*
Mortality
Myocardial Infarction
Rats*
Rats, Sprague-Dawley
Minocycline