Korean J Phys Anthropol.  2004 Sep;17(3):231-240.

Down-regulated Reactive Oxygen Species by Heat Shock Protein 90 in 3-Hydroxykynurenine-induced SKN-SH Cell Death

• Kim DS
• Lee MW
• Noh YH
• Lee DY
• Lee HJ
• Bach JH
• Han JW
• Lee KG
• Yeo JH
• Lee WB
• Kim SS

Abstract

In this present study, we show that 3HK-induced reactive oxygen species (ROS) accumulation and caspase activation lead to apoptotic cell death. Pretreatment with N-acetylcysteine (NAC), an effective antioxidant, significantly attenuated 3HK-induced apoptosis by way of a reduction of ROS accumulation and caspase activity. SKN-SN cells were protected from 3HK-induced cytotoxicity by heat shock protein (HSP). HSP90 effectively attenuated 3HK-mediated ROS accumulation and apoptosis. In addition, the protective effect of HSP90 was abolished by pretreatment with HSP90 anti-sense oligonucleotide, but not when pretreated with anti-senses for other HSPs. These results suggest that HSP90 protects SKN-SH cells from 3HK-induced cytotoxicity by reducing ROS levels and caspase activity.

Keyword

3-Hydroxykynurenine (3HK); Heat shock protein 90(HSP90); Reactive oxygen species (ROS); Caspase; Apoptosis

MeSH Terms

Acetylcysteine
Apoptosis
Cell Death*
Heat-Shock Proteins*
Hot Temperature*
Reactive Oxygen Species*
Acetylcysteine
Heat-Shock Proteins
Reactive Oxygen Species