J Korean Soc Microbiol.  1997 Jun;32(3):325-334.

Isolation and Identification of the Antigen Recognized by Human Cytomegalovirus Specific Monoclonal Antibody SCMVM 34

• Park CG
• Kook YH
• Cha CY
• Hwang ES
• Lim DG
• Seoh JY
• Park JW
• Jong HS

Abstract

Human cytomegalovirus (HCMV)-specific monoclonal antibody, SCMVM 34, recognizes early antigen confined to the nucleus of HCMV-infected cells. This study was performed to identify the antigen reactive to SCMVM 34 with purification and amino acid sequencing. The nuclear and cytoskeletal fraction of HCMV-infected cells was subjected to 0.4 M NaCl extraction, DEAE-Sephacel ion exchange chromatography, DNA-cellulose chromatography and SDS-PAGE. The molecular weight of the reactive proteins was 52 kD, 40 kD and 34 kD. The modified or blocked amino termini of 52 kD and 40 kD showed resistance to Edman degradation. The internal peptide fragments were isolated by tryptic digeytion and reverse-phase HPLC. The internal amino acid sequence analysis of the peptides from HPLC profile revealed that the antigens recognized by SCMVM 34 was ppUIA4.

MeSH Terms

Chromatography
Chromatography, High Pressure Liquid
Chromatography, Ion Exchange
Cytomegalovirus*
Electrophoresis, Polyacrylamide Gel
Humans*
Molecular Weight
Peptide Fragments
Peptides
Sequence Analysis, Protein
Peptide Fragments
Peptides