Korean J Parasitol.
2013 Apr;51(2):197-201. 10.3347/kjp.2013.51.2.197.
Molecular Characterization of Taenia multiceps Isolates from Gansu Province, China by Sequencing of Mitochondrial Cytochrome C Oxidase Subunit 1
- Affiliations
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- 1State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Public Health of the Ministry of Agriculture, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agric
- 2College of Veterinary Medicine, Gansu Agricultural University, 730070, Lanzhou, China.
- 3JRU BIPAR ENVA ANSES UPEC, 94703 Maisons-Alfort Cedex, France.
- KMID: 1499698
- DOI: http://doi.org/10.3347/kjp.2013.51.2.197
Abstract
- A total of 16 Taenia multiceps isolates collected from naturally infected sheep or goats in Gansu Province, China were characterized by sequences of mitochondrial cytochrome c oxidase subunit 1 (cox1) gene. The complete cox1 gene was amplified for individual T. multiceps isolates by PCR, ligated to pMD18T vector, and sequenced. Sequence analysis indicated that out of 16 T. multiceps isolates 10 unique cox1 gene sequences of 1,623 bp were obtained with sequence variation of 0.12-0.68%. The results showed that the cox1 gene sequences were highly conserved among the examined T. multiceps isolates. However, they were quite different from those of the other Taenia species. Phylogenetic analysis based on complete cox1 gene sequences revealed that T. multiceps isolates were composed of 3 genotypes and distinguished from the other Taenia species.
MeSH Terms
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Animals
China
Cluster Analysis
Cysticercosis/parasitology/veterinary
DNA, Helminth/chemistry/genetics/isolation & purification
DNA, Mitochondrial/chemistry/genetics/isolation & purification
Electron Transport Complex IV/*genetics
*Genetic Variation
Goat Diseases/parasitology
Goats
Phylogeny
Polymerase Chain Reaction
Protein Subunits/genetics
Sequence Analysis, DNA
Sheep
Sheep Diseases/parasitology
Taenia/*classification/genetics/*isolation & purification
DNA, Helminth
DNA, Mitochondrial
Protein Subunits
Electron Transport Complex IV
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