J Korean Med Sci.  2007 Aug;22(4):667-671. 10.3346/jkms.2007.22.4.667.

Lack of Association between Matrix Metalloproteinase 8 Promoter Polymorphism and Bronchiectasis in Koreans

• Lee J
• Kim HR
• Min JW
• Park JS
• Jin SM
• Han SK
• Shim YS
• Yim JJ

• KMID: 1127084
• DOI: http://doi.org/10.3346/jkms.2007.22.4.667

Abstract

The observation that human matrix metalloproteinase (MMP)-8 is over-expressed in ectatic bronchi in patients with bronchiectasis suggests that polymorphisms altering the expression of MMP-8 may contribute to the susceptibility to development of bronchiectasis. We evaluated the association between the presence of bronchiectasis in a Korean population and two single nucleotide polymorphisms (SNPs) (-799C/T and -381A/G) on the promoter region of the MMP-8 gene that are reported to alter the promoter activity and thereby the gene expression. Genotyping through polymerase chain reaction (PCR) and subsequent automatic sequencing was done in 167 patients with bronchiectasis and their age-, sex-matched healthy controls to reveal that only -799C/T is polymorphic among Koreans. In the patient group with bronchiectasis, the frequency of -799C/C, C/T, and T/T genotypes were 41.9%, 49.7%, and 8.4%, respectively. A similar distribution was observed in the control group: C/C (49.7%), C/T (43.1%), and T/T (7.2%) (p=0.36). In subgroup analysis, no significant difference was observed among the patients according to; the extent of disease (p=0.76), colonization of microorganisms (p=0.56), or association of mycobacteria (p=0.17). From these results, we conclude that -799C/T on the promoter region of MMP-8 lacks association with development of bronchiectasis in Koreans.

Keyword

Bronchiectasis; Genetic Predisposition to Disease; Koreans; Matrix-metalloproteinase; Neutrophil Collagenase; Single Nucleotide Polymorphism

MeSH Terms

Adult
Aged
Aged, 80 and over
Alleles
Asian Continental Ancestry Group/*genetics
Bronchiectasis/enzymology/ethnology/*genetics
Female
Gene Frequency
Genotype
Humans
Korea
Male
Matrix Metalloproteinase 8/*genetics
Middle Aged
Odds Ratio
*Polymorphism, Single Nucleotide
Promoter Regions, Genetic/*genetics

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