Korean J Ophthalmol.  2005 Mar;19(1):1-8. 10.3341/kjo.2005.19.1.1.

Effect of Basic Fibroblast Growth Factor on Fibrovascular Ingrowth into Porous Polyethylene Anophthalmic Socket Implants

• Park WC
• Han SK
• Kim NJ
• Chung TY
• Khwarg SI

• KMID: 1099078
• DOI: http://doi.org/10.3341/kjo.2005.19.1.1

Abstract

To investigate the effect of basic Fibroblast Growth Factor (bFGF) on fibrovascular ingrowth into porous polyethylene orbital implants (Medpor (R) ) and to investigate any differences according to the method of administration. For the treated groups, after evisceration and Medpor (R) implantation, bFGF was administered by soaking Medpor (R) in the bFGF solution, and/or by injecting bFGF into the Medpor (R) 1 week after the operation. Implants were removed 4 weeks after the operation and examined for the degrees of fibrovascular ingrowth by light microscopy. The percentages of the cross-sectional area of the implant occupied by fibrovascular ingrowth and the numbers of proliferated vessels were significantly higher in the bFGF-treated groups (Mann Whitney test, p< 0.05). Administration routes had no effect on the degree of fibrovascular ingrowth (Kruskal-Wallis test, p> 0.05). bFGF promoted fibrovascular ingrowth into porous polyethylene orbital implants regardless of the route of administration. Therefore, bFGF might be helpful to prevent complications such as implant exposure.

Keyword

Basic fibroblast growth factor; Fibrovascular ingrowth; Porous polyethylene orbital implant

MeSH Terms

Animals
Eye Evisceration
Fibroblast Growth Factor 2/administration & dosage/*pharmacology
Fibroblasts/*drug effects
Neovascularization, Physiologic/*drug effects
*Orbital Implants
*Polyethylene
Porosity
Prosthesis Implantation
Rabbits
Research Support, Non-U.S. Gov't

Figure

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  Fig. 1 Determination of fibrovascular ingrowth margins in H&E-stained pathologic specimens under a light microscope. (A) Low magnification view (10×) of the cross section of the implant. (B) Higher magnification view (100×) of the box area in Figure 1A. A connective tissue enmeshed with spindle-shaped fibroblasts can be seen on the left side of the picture. Its inner border was drawn with a dotted line. An inner myxoid area with round-shaped cells and rare spindle-shaped fibroblasts (the area right to the dotted line) was not interpreted as an ingrown fibrovascular tissue.

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  Fig. 2 Area measuring by Sigmascan pro®.

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  Fig. 3 Counting of proliferated vessels. (A) After immunohistochemical staining for vascular endothelial cells using CD31 antibodies, five areas were randomly selected for counting. (B) Proliferated vessels were evident in the proliferated fibrous tissue by endothelial cells staining with CD31 antibodies (×100). Vessels within one examining field at 100× were counted.

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