Clin Exp Vaccine Res.  2016 Jan;5(1):26-30. 10.7774/cevr.2016.5.1.26.

Comparison of the antigenic relationship between Japanese encephalitis virus genotypes 1 and 3

• Kang BK
• Hwang JM
• Moon H
• Han SY
• Kim JM
• Yang DK
• Park BK
• Song D

• KMID: 2152694
• DOI: http://doi.org/10.7774/cevr.2016.5.1.26

Abstract

PURPOSE
The Japanese encephalitis virus (JEV) genotype circulating in Korea has changed from G3 to G1. Therefore, the purpose of this study was to compare the antigenic relationship between the two genotypes by using antibody tests.
MATERIALS AND METHODS
Blood samples from 42 sows and 216 horses were collected, and their seroprevalence was monitored using the hemagglutination inhibition and virus neutralization tests. Antisera against JEV G1 and G3 were isolated and prepared from guinea pigs. The cross-reactivity of these two viruses was then compared using the neutralizing antibody test.
RESULTS
We found that there was a difference in the seropositive ratios of JEV G1 and G3. However, the difference was dependent on the antibody test used. There was also an observed difference in the antigenicity between the two genotypes, as ascertained using the neutralizing antibody test.
CONCLUSION
There is an evident difference in JEV antigenicity between the genotypes G1 and G3. Therefore, we propose monitoring of the seroprevalence of JEV, and reevaluating the antigenicity of the current vaccine by using the relevant tests.

Keyword

Japanese encephalitis virus; Genotype; Seroprevalence; Cross reaction

MeSH Terms

Animals
Antibodies, Neutralizing
Asian Continental Ancestry Group*
Cross Reactions
Encephalitis Virus, Japanese*
Encephalitis, Japanese*
Genotype
Guinea Pigs
Hemagglutination
Horses
Humans
Immune Sera
Korea
Neutralization Tests
Seroepidemiologic Studies
Antibodies, Neutralizing
Immune Sera

Reference

1. Gubler DJ. The continuing spread of West Nile virus in the western hemisphere. Clin Infect Dis. 2007; 45:1039–1046.

2. Erlanger TE, Weiss S, Keiser J, Utzinger J, Wiedenmayer K. Past, present, and future of Japanese encephalitis. Emerg Infect Dis. 2009; 15:1–7.

3. Han N, Adams J, Chen P, et al. Comparison of genotypes I and III in Japanese encephalitis virus reveals distinct differences in their genetic and host diversity. J Virol. 2014; 88:11469–11479.

4. Schuh AJ, Ward MJ, Brown AJ, Barrett AD. Phylogeography of Japanese encephalitis virus: genotype is associated with climate. PLoS Negl Trop Dis. 2013; 7:e2411.

5. Schuh AJ, Ward MJ, Leigh Brown AJ, Barrett AD. Dynamics of the emergence and establishment of a newly dominant genotype of Japanese encephalitis virus throughout Asia. J Virol. 2014; 88:4522–4532.

6. Shimojima M, Nagao Y, Shimoda H, et al. Full genome sequence and virulence analyses of the recent equine isolate of Japanese encephalitis virus. J Vet Med Sci. 2011; 73:813–816.

7. Yang DK, Kim HH, Nah JJ, Lee KW, Song JY. Binary ethylenimine inactivated Japanese encephalitis virus antigen reveals hemagglutination. Open J Vet Med. 2012; 2:120–123.

8. Yang DK, Nah JJ, Kim HH, Song JY. Inactivated genotype 1 Japanese encephalitis vaccine for swine. Clin Exp Vaccine Res. 2014; 3:212–219.

9. Yang DK, Kim BH, Kweon CH, et al. Serosurveillance for Japanese encephalitis, Akabane, and Aino viruses for Thoroughbred horses in Korea. J Vet Sci. 2008; 9:381–385.

10. Lindenbach B, Rice C. Flaviviridae: the viruses and their replication. In : Fields BN, Howley PM, Griffin DE, Lamb RA, Martin MA, Roizman B, editors. Fields virology. 4th ed. Philadelphia: Lippincott Williams & Wilkins;2001. p. 991–1041.

11. Chen WR, Tesh RB, Rico-Hesse R. Genetic variation of Japanese encephalitis virus in nature. J Gen Virol. 1990; 71(Pt 12):2915–2922.

12. Wang HY, Takasaki T, Fu SH, et al. Molecular epidemiological analysis of Japanese encephalitis virus in China. J Gen Virol. 2007; 88(Pt 3):885–894.

13. Nitatpattana N, Dubot-Peres A, Gouilh MA, et al. Change in Japanese encephalitis virus distribution, Thailand. Emerg Infect Dis. 2008; 14:1762–1765.

14. Nga PT, del Carmen Parquet M, Cuong VD, et al. Shift in Japanese encephalitis virus (JEV) genotype circulating in northern Vietnam: implications for frequent introductions of JEV from Southeast Asia to East Asia. J Gen Virol. 2004; 85(Pt 6):1625–1631.

15. Yun SM, Cho JE, Ju YR, et al. Molecular epidemiology of Japanese encephalitis virus circulating in South Korea, 1983-2005. Virol J. 2010; 7:127.

16. Yamanaka T, Tsujimura K, Kondo T, et al. Isolation and genetic analysis of Japanese encephalitis virus from a diseased horse in Japan. J Vet Med Sci. 2006; 68:293–295.

17. Lee NS, Mun JB, Kim YH, Song KC. Studies on Japanese encephalitis. VI. Survey of incidence of the antibodies against Japanese encephalitis virus among domestic animals. Res Rep Natl Inst Vet Res. 1956; 4:21–38.

18. Yang DK, Kim BH, Kweon CH, Kwon JH, Lim SI, Han HR. Biophysical characterization of Japanese encephalitis virus (KV1899) isolated from pigs in Korea. J Vet Sci. 2004; 5:125–130.

19. Yang DK, Kweon CH, Kim BH, et al. The seroprevalence of Japanese encephalitis virus in goats raised in Korea. J Vet Sci. 2007; 8:197–199.

20. Fan YC, Chen JM, Chen YY, Lin JW, Chiou SS. Reduced neutralizing antibody titer against genotype I virus in swine immunized with a live-attenuated genotype III Japanese encephalitis virus vaccine. Vet Microbiol. 2013; 163:248–256.

21. Westaway EG. Flavivirus replication strategy. Adv Virus Res. 1987; 33:45–90.

22. Yang DK, Kim BH, Kweon CH, Kwon JH, Lim SI, Han HR. Molecular characterization of full-length genome of Japanese encephalitis virus (KV1899) isolated from pigs in Korea. J Vet Sci. 2004; 5:197–205.

23. Ku CC, King CC, Lin CY, et al. Homologous and heterologous neutralization antibody responses after immunization with Japanese encephalitis vaccine among Taiwan children. J Med Virol. 1994; 44:122–131.