Korean J Vet Res.  2012 Jun;52(2):89-97.

Immunostimulatory effects of BCG-CWS on the proliferation and viability of mouse spleen cells

Affiliations
  • 1Laboratory of Veterinary Pharmacology, College of Veterinary Medicine, Jeju National University, Jeju 690-756, Korea. jooh@jejunu.ac.kr

Abstract

Mycobacterial cell-wall skeleton (CWS) is an immunoactive and biodegradable particulate adjuvant and has been tried to use for immunotherapy. The CWS of Mycobacterium bovis bacillus Calmette-Guerin (BCG-CWS) was studied as an universal vaccine vehicle for antigen conjugation, to develop potentially effective and safe vaccine. Although a variety of biological activities of BCG-CWS have been studied, the effects of BCG-CWS on spleen cells are not fully elucidated. Using MTT assay and trypan blue exclusion test, we found that BCG-CWS significantly enhanced the viability and proliferation of cells. Multiple clusters, indicating proliferation, were observed in BCG-CWS-treated spleen cells and surface marker staining assay revealed that BCG-CWS promoted the proliferation of CD19+ B lymphocyte rather than CD4+ or CD8+ T lymphocyte. In addition, BCG-CWS up-regulated the expression of anti-apoptotic molecules such as bcl-2, bcl-xL. BCG-CWS increased the surface expression of CD25 and CD69 as well as IL-2 production of spleen cells, suggesting increased activation. Furthermore, BCG-CWS enhanced the antigen-specific cell proliferation and interferon-gamma production of spleen cells. Taken together, these results demonstrate the immunostimulatory effects of BCG-CWS on spleen cells via multiple mechanisms, providing valuable information to broaden the use of BCG-CWS in clinical and research settings.

Keyword

adjuvant; BCG-CWS; spleen cell; vaccine

MeSH Terms

Animals
Bacillus
Cell Proliferation
Collodion
Diminazene
Immunotherapy
Interferon-gamma
Interleukin-2
Lymphocytes
Mice
Mycobacterium bovis
Skeleton
Spleen
Trypan Blue
Collodion
Diminazene
Interferon-gamma
Interleukin-2
Trypan Blue
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