Lab Anim Res.  2010 Sep;26(3):273-278.

Tissue Specific Expression of Lipid Metabolism Related Molecules in Digestive Organs of Miniature Pigs

• Han HJ
• Kang SS
• Park SH

Abstract

The miniature pig is a very suitable donor species in xenotransplantation of human organs. Lipid metabolism is an important process that involves the creation and degradation of lipids, which is associated with the function of the gastro-intestinal tract. However, the distribution of lipid metabolism related molecules in the gastro-intestinal tract in the miniature pig is unclear. The present study examined the expression of farnesoid X-receptor (FXR), liver X- receptor (LXR), retinoid X-receptor (RXR), liver fatty acid binding protein (L-FABP), fatty acid synthase (FAS) mRNA in the digestive organs of miniature pigs. FXR and LXR mRNA were not expressed in the stomach but were expressed at high and low density in the small and large intestines, respectively. RXR mRNA was expressed in stomach with moderate density, small intestine with high density and in the large intestine with low density. L-FABP and FAS mRNA were expressed in the stomach and large intestine with low density and in the small intestine with high density. L-FABP mRNA was expressed in the liver and kidney with high density, and in pancreas with low density. FAS mRNA was expressed in the liver with high density, and in pancreas and kidney with low density.

Keyword

Miniature pigs; lipid metabolism related molecules; gastro-intestinal tract; mRNA

MeSH Terms

Fatty Acid Synthetase Complex
Fatty Acid-Binding Proteins
Humans
Intestine, Large
Intestine, Small
Intestines
Kidney
Lipid Metabolism
Liver
Pancreas
RNA, Messenger
Stomach
Swine
Tissue Donors
Transplantation, Heterologous
Fatty Acid Synthetase Complex
Fatty Acid-Binding Proteins
RNA, Messenger

Figure

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  Figure 1. The expression of FXR, LXR, RXR-beta, L-FABP, and FAS mRNA in the fundus, body and antrum of stomach in miniature pigs (A). mRNA expression was evaluated by RT-PCR as described in Materials and Methods nsection. Beta-actin was used as an endogenous control. Lower panel depicts the relative contents of optimal density in stomach (B).

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  Figure 2. The expression of FXR, LXR, RXR-beta, L-FABP, and FAS mRNA in the small intestine (duodenum, jejunum and ileum) and large intestine (cecum, colon and rectum) in miniature pigs (A). Lower panel depicts the relative contents of optimal density in small intestine (B) and large intestine (C).

• 

  Figure 3. The expression of FXR, LXR, RXR-beta, L-FABP, and FAS mRNA in the liver, pancreas, and kidney in miniature pigs (A). Lower panel depicts the relative contents of optimal density in liver, kidney, pancreas, and kidney (B).

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