J Lung Cancer.  2005 Jun;4(1):38-41.

Mutational Analysis of Caspase-7 and 8 Genes in Non-small Cell Lung Cancer

Affiliations
  • 1Department of Pathology, College of Medicine, The Catholic University of Korea, Seoul, Korea. suhulee@catholic.ac.kr
  • 2Department of Thoracic Surgery, College of Medicine, The Catholic University of Korea, Seoul, Korea.

Abstract

PURPOSE : Several lines of evidence have indicated that deregulation of apoptosis is involved in the mechanism of cancer development. Caspase-8 activation plays a central role in the initiation phase of apoptosis, while caspase-7 is one of the main execution phase caspases of apoptosis. The aim of this study was to explore the possibility that genetic alterations of the caspase-8 and caspase-7 genes are involved in the development of human non-small cell lung cancer (NSCLC).
MATERIALS AND METHODS
: We have analyzed the entire coding region of both the caspase-7 and caspase-8 genes to detect the somatic mutations in 100 NSCLCs by using polymerase chain reaction (PCR)- single strand conformation polymorphism (SSCP).
RESULTS
: The PCR-SSCP analysis detected no mutations in the entire coding regions of both the caspase-7 and caspase-8 genes in the NSCLCs.
CONCLUSION
: The data presented here suggests that both the caspase-7 and caspase-8 genes may not be somatically mutated in human NSCLCs

Keyword

Non-small cell lung cancer; Caspase-7; Caspase-8; Apoptosis; Mutation

MeSH Terms

Apoptosis
Carcinoma, Non-Small-Cell Lung*
Caspase 7*
Caspase 8
Caspases
Clinical Coding
Humans
Polymerase Chain Reaction
Caspase 7
Caspase 8
Caspases
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