Korean J Nutr.  2013 Apr;46(2):119-125. 10.4163/kjn.2013.46.2.119.

Effects of medicinal herb water extracts on expression of hepatic glucokinase, pyruvate dehydrogenase and acetyl-CoA carboxylase mRNA

• Kim HS
• Kim TW
• Kim DJ
• Lee JS
• Choe M

• KMID: 2269389
• DOI: http://doi.org/10.4163/kjn.2013.46.2.119

Abstract

We studied the anti-diabetic effects of medicinal herb water extracts on expression of hepatic glucokinase (GCK), pyruvate dehydrogenase (PDH), and acetyl-CoA carboxylase (ACC) mRNA. The medicinal herbs used for experiments were Cornus officinalis (CO), Paeonia suffruticosa Andrews (PSA), Discorea japonica Thunb. (DJ), Rehmannia glutinosa (RG), Lycium chinense (LC), and Pyrus pyrifolia (PP). For GCK mRNA expression, CO, RG, and LC water extracts exhibited a more effective activity than other extracts. Cells treated with RG and LC water extracts showed an increase in expression of PDH mRNA to 191% and 124%, respectively, compared to control. Expression of ACC mRNA was significantly higher in LC water extract. These data indicate that CO, RG, and LC water extracts stimulates expression of hepatic GCK, PDH, and ACC mRNA.

Keyword

anti-diabetic; glucokinase; pyruvate dehydrogenase; acetyl-CoA carboxylase; medicinal herb

MeSH Terms

Acetyl Coenzyme A
Acetyl-CoA Carboxylase
Cornus
Glucokinase
Lycium
Oxidoreductases
Paeonia
Plants, Medicinal
Pyrus
Pyruvic Acid
Rehmannia
RNA, Messenger
Water
Acetyl Coenzyme A
Acetyl-CoA Carboxylase
Glucokinase
Oxidoreductases
Pyruvic Acid
RNA, Messenger
Water

Figure

• 

  Fig. 1 Effect of medicinal herb water extracts on the cell viability in HepG2 cells. Results are from three experiments and expressed as Mean ± SE (n = 3). Means with the different letters are significantly different by Duncan's multiple range test (p < 0.05). CO: Cornus officinalis, PSA: Paeonia suffruticosa Andrews, DJ: Discorea japonica Thunb, RG: Rehmannia glutinosa, LC: Lycium chinense, PP: Pyrus pyrifolia.

• 

  Fig. 2 Glucokinase mRNA expression of the water extracts from medicinal herbs. Results are from three experiments and expressed as Mean ± SE (n = 3). Means with the different letters are significantly different by Duncan's multiple range test (p < 0.05). CO: Cornus officinalis, PSA: Paeonia suffruticosa Andrews, DJ: Discorea japonica Thunb, RG: Rehmannia glutinosa, LC: Lycium chinense, PP: Pyrus pyrifolia.

• 

  Fig. 3 Pyruvate dehydrogenase mRNA expression of the water extracts from medicinal herbs. Results are from three experiments and expressed as Mean ± SE (n = 3). Means with the different letters are significantly different by Duncan's multiple range test (p < 0.05). CO: Cornus officinalis, PSA: Paeonia suffruticosa Andrews, DJ: Discorea japonica Thunb, RG: Rehmannia glutinosa, LC: Lycium chinense, PP: Pyrus pyrifolia.

• 

  Fig. 4 Acetyl-CoA carboxylase mRNA expression of the water extracts from medicinal herbs. Results are from three experiments and expressed as Mean ± SE (n = 3). Means with the different letters are significantly different by Duncan's multiple range test (p < 0.05). CO: Cornus officinalis, PSA: Paeonia suffruticosa Andrews, DJ: Discorea japonica Thunb, RG: Rehmannia glutinosa, LC: Lycium chinense, PP: Pyrus pyrifolia.

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