J Vet Sci.  2008 Sep;9(3):301-308. 10.4142/jvs.2008.9.3.301.

Protection of chickens from Newcastle disease with a recombinant baculovirus subunit vaccine expressing the fusion and hemagglutininneuraminidase proteins

• Lee YJ
• Sung HW
• Choi JG
• Lee EK
• Yoon H
• Kim JH
• Song CS

• KMID: 1718584
• DOI: http://doi.org/10.4142/jvs.2008.9.3.301

Abstract

Recombinant baculoviruses containing the fusion (F) and hemagglutinin-neuraminidase (HN) glycoprotein gene of the viscerotropic velogenic (vv) Newcastle disease virus (NDV) isolate, Kr-005/00, and a lentogenic La Sota strain of the NDV were constructed in an attempt to develop an effective subunit vaccine to the recent epizootic vvNDV. The level of protection was determined by evaluating the clinical signs, mortality, and virus shedding from the oropharynx and cloaca of chickens after a challenge with vvNDV Kr-005/00. The recombinant ND F (rND F) and recombinant HN (rND HN) glycoproteins derived from the velogenic strain provided good protection against the clinical signs and mortality, showing a 0.00 PI value and 100% protection after a booster immunization. On the other hand, the combined rND F + HN glycoprotein derived from the velogenic strain induced complete protection (0.00 PI value and 100% protection) and significantly reduced the amount of virus shedding even after a single immunization. The rND F and rND HN glycoproteins derived from the velogenic strain had a slightly, but not significantly, greater protective effect than the lentogenic strain. These results suggest that the combined rND F + HN glycoprotein derived from vvNDV can be an ideal subunit marker vaccine candidate in chickens in a future ND eradication program.

Keyword

Newcastle disease; recombinant protein; subunit vaccine

MeSH Terms

Animals
Baculoviridae/genetics/*immunology
Chickens/*virology
DNA Primers
Gene Amplification
HN Protein/genetics/*therapeutic use
Korea
Marek Disease/immunology/prevention & control
Newcastle Disease/immunology/*prevention & control
Spodoptera/virology
Vaccines, Synthetic/genetics/therapeutic use
Viral Vaccines/genetics/therapeutic use

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