Korean J Parasitol.  2008 Dec;46(4):279-283. 10.3347/kjp.2008.46.4.279.

Antibody Responses in Sera of Different Mouse Strains Experimentally Infected with Neodiplostomum seoulense

Affiliations
  • 1Department of Parasitology, Kangwon National University College of Medicine, Chuncheon 200-701, Korea.
  • 2Institute of Parasitic Diseases, Zhejiang Academy of Medical Sciences, Hangzhou 310013, Zhejiang Province, P.R. China.
  • 3Department of Parasitology and Tropical Medicine, Seoul National University College of Medicine, and Institute of Endemic Diseases, Seoul National University Medical Research Center, Seoul 110-799, Korea. cjy@snu.ac.kr

Abstract

To examine humoral immune responses in the host, we measured serum antibody levels in different strains of mice (ICR, BALB/c, and C3H) experimentally infected with Neodiplostomum seoulense. Specific IgG antibody levels were increased remarkably with little difference among 3 strains of mice infected with N. seoulense from day 7 to 35 post-infection. More target proteins of adult parasites reacted with IgG at the time when the worm recovery decreased compared with other times. More than 20 protein bands, from 14 kDa to 94 kDa in size, were separated from the crude antigen of N. seoulense adults by SDS-PAGE, and among them 26, 30, 35, 43, 54, 67, and 94 kDa proteins were the major antigenic proteins. The results suggest that significant IgG antibody responses occur against N. seoulense in mice and this may be related with expulsion of worms.

Keyword

Neodiplostomum seoulense; immunoglobulin G; immune response; mouse; infection

MeSH Terms

Animals
Antibodies, Helminth/*blood
Host-Parasite Interactions
Mice
Mice, Inbred BALB C
Mice, Inbred C3H
Mice, Inbred ICR
Trematoda/classification
Trematode Infections/*blood/*immunology
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